The HIV Lipidome: A Raft with an Unusual Composition
The lipids of enveloped viruses play critical roles in viral morphogenesis and infectivity. They are derived from the host membranes from which virus budding occurs, but the precise lipid composition has not been determined for any virus. Employing mass spectrometry, this study provides a quantitati...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2006-02, Vol.103 (8), p.2641-2646 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2646 |
|---|---|
| container_issue | 8 |
| container_start_page | 2641 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 103 |
| creator | Brügger, Britta Glass, Bärbel Haberkant, Per Leibrecht, Iris Wieland, Felix T. Kräusslich, Hans-Georg |
| description | The lipids of enveloped viruses play critical roles in viral morphogenesis and infectivity. They are derived from the host membranes from which virus budding occurs, but the precise lipid composition has not been determined for any virus. Employing mass spectrometry, this study provides a quantitative analysis of the lipid constituents of HIV and a comprehensive comparison with its host membranes. Both a substantial enrichment of the unusual sphingolipid dihydrosphingomyelin and a loss of viral infectivity upon inhibition of sphingolipid biosynthesis in host cells are reported, establishing a critical role for this lipid class in the HIV replication cycle. Intriguingly, the overall lipid composition of native HIV membranes resembles detergent-resistant membrane microdomains and is strikingly different from that of host cell membranes. With this composition, the HIV lipidome provides strong evidence for the existence of lipid rafts in living cells. |
| doi_str_mv | 10.1073/pnas.0511136103 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1413831</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>30049466</jstor_id><sourcerecordid>30049466</sourcerecordid><originalsourceid>FETCH-LOGICAL-c526t-a197ba2a83f9d6d4948dc2f4bae3adf8ec24c767cb3786f3ecdb4bf6b2b21de73</originalsourceid><addsrcrecordid>eNqF0c9rFDEUB_Agil2rZ0_K4KGepn0vyeaHB6EsagsLgrReQyaTuFlmJuNkRu1_7yy7dKsInnLI5315yZeQlwjnCJJd9J3N57BERCYQ2COyQNBYCq7hMVkAUFkqTvkJeZbzFgD0UsFTcoKCKxSULgi_2fji6vprsY59rFPr3xWXxRcbxuJnHDeF7YrbbsqTbYpVavuU4xhT95w8CbbJ_sXhPCW3Hz_crK7K9edP16vLdemWVIylRS0rS61iQdei5pqr2tHAK-uZrYPyjnInhXQVk0oE5l1d8SqIilYUay_ZKXm_z-2nqvW189042Mb0Q2ztcGeSjebPmy5uzLf0wyBHphjOAWeHgCF9n3weTRuz801jO5-mbITUVAgF_4UoQeFS6Rm--Qtu0zR08y8YCsgpML3b-2KP3JByHny4XxnB7Hozu97Msbd54vXDlx79oagZvD2A3eQxjhllqOBowtQ0o_81Poj6t5zBqz3Y5jEN94IBzA0JwX4DPFW0lg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>201420397</pqid></control><display><type>article</type><title>The HIV Lipidome: A Raft with an Unusual Composition</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Brügger, Britta ; Glass, Bärbel ; Haberkant, Per ; Leibrecht, Iris ; Wieland, Felix T. ; Kräusslich, Hans-Georg</creator><creatorcontrib>Brügger, Britta ; Glass, Bärbel ; Haberkant, Per ; Leibrecht, Iris ; Wieland, Felix T. ; Kräusslich, Hans-Georg</creatorcontrib><description>The lipids of enveloped viruses play critical roles in viral morphogenesis and infectivity. They are derived from the host membranes from which virus budding occurs, but the precise lipid composition has not been determined for any virus. Employing mass spectrometry, this study provides a quantitative analysis of the lipid constituents of HIV and a comprehensive comparison with its host membranes. Both a substantial enrichment of the unusual sphingolipid dihydrosphingomyelin and a loss of viral infectivity upon inhibition of sphingolipid biosynthesis in host cells are reported, establishing a critical role for this lipid class in the HIV replication cycle. Intriguingly, the overall lipid composition of native HIV membranes resembles detergent-resistant membrane microdomains and is strikingly different from that of host cell membranes. With this composition, the HIV lipidome provides strong evidence for the existence of lipid rafts in living cells.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0511136103</identifier><identifier>PMID: 16481622</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Biological Sciences ; Cell membranes ; Cells, Cultured ; Cellular biology ; Chemical composition ; Cholesterols ; HIV ; HIV 1 ; HIV-1 - chemistry ; HIV-1 - pathogenicity ; Human immunodeficiency virus ; Humans ; Lipids ; Mass Spectrometry ; Membrane Microdomains - chemistry ; Membranes ; P branes ; Phospholipids - analysis ; Rafts ; Sphingolipids ; Sphingolipids - analysis ; Sphingolipids - biosynthesis ; T-Lymphocytes - virology ; Viruses</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2006-02, Vol.103 (8), p.2641-2646</ispartof><rights>Copyright 2006 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Feb 21, 2006</rights><rights>2006 by The National Academy of Sciences of the USA 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-a197ba2a83f9d6d4948dc2f4bae3adf8ec24c767cb3786f3ecdb4bf6b2b21de73</citedby><cites>FETCH-LOGICAL-c526t-a197ba2a83f9d6d4948dc2f4bae3adf8ec24c767cb3786f3ecdb4bf6b2b21de73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/103/8.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30049466$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30049466$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,315,728,781,785,804,886,27929,27930,53796,53798,58022,58255</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16481622$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brügger, Britta</creatorcontrib><creatorcontrib>Glass, Bärbel</creatorcontrib><creatorcontrib>Haberkant, Per</creatorcontrib><creatorcontrib>Leibrecht, Iris</creatorcontrib><creatorcontrib>Wieland, Felix T.</creatorcontrib><creatorcontrib>Kräusslich, Hans-Georg</creatorcontrib><title>The HIV Lipidome: A Raft with an Unusual Composition</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The lipids of enveloped viruses play critical roles in viral morphogenesis and infectivity. They are derived from the host membranes from which virus budding occurs, but the precise lipid composition has not been determined for any virus. Employing mass spectrometry, this study provides a quantitative analysis of the lipid constituents of HIV and a comprehensive comparison with its host membranes. Both a substantial enrichment of the unusual sphingolipid dihydrosphingomyelin and a loss of viral infectivity upon inhibition of sphingolipid biosynthesis in host cells are reported, establishing a critical role for this lipid class in the HIV replication cycle. Intriguingly, the overall lipid composition of native HIV membranes resembles detergent-resistant membrane microdomains and is strikingly different from that of host cell membranes. With this composition, the HIV lipidome provides strong evidence for the existence of lipid rafts in living cells.</description><subject>Biological Sciences</subject><subject>Cell membranes</subject><subject>Cells, Cultured</subject><subject>Cellular biology</subject><subject>Chemical composition</subject><subject>Cholesterols</subject><subject>HIV</subject><subject>HIV 1</subject><subject>HIV-1 - chemistry</subject><subject>HIV-1 - pathogenicity</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Lipids</subject><subject>Mass Spectrometry</subject><subject>Membrane Microdomains - chemistry</subject><subject>Membranes</subject><subject>P branes</subject><subject>Phospholipids - analysis</subject><subject>Rafts</subject><subject>Sphingolipids</subject><subject>Sphingolipids - analysis</subject><subject>Sphingolipids - biosynthesis</subject><subject>T-Lymphocytes - virology</subject><subject>Viruses</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c9rFDEUB_Agil2rZ0_K4KGepn0vyeaHB6EsagsLgrReQyaTuFlmJuNkRu1_7yy7dKsInnLI5315yZeQlwjnCJJd9J3N57BERCYQ2COyQNBYCq7hMVkAUFkqTvkJeZbzFgD0UsFTcoKCKxSULgi_2fji6vprsY59rFPr3xWXxRcbxuJnHDeF7YrbbsqTbYpVavuU4xhT95w8CbbJ_sXhPCW3Hz_crK7K9edP16vLdemWVIylRS0rS61iQdei5pqr2tHAK-uZrYPyjnInhXQVk0oE5l1d8SqIilYUay_ZKXm_z-2nqvW189042Mb0Q2ztcGeSjebPmy5uzLf0wyBHphjOAWeHgCF9n3weTRuz801jO5-mbITUVAgF_4UoQeFS6Rm--Qtu0zR08y8YCsgpML3b-2KP3JByHny4XxnB7Hozu97Msbd54vXDlx79oagZvD2A3eQxjhllqOBowtQ0o_81Poj6t5zBqz3Y5jEN94IBzA0JwX4DPFW0lg</recordid><startdate>20060221</startdate><enddate>20060221</enddate><creator>Brügger, Britta</creator><creator>Glass, Bärbel</creator><creator>Haberkant, Per</creator><creator>Leibrecht, Iris</creator><creator>Wieland, Felix T.</creator><creator>Kräusslich, Hans-Georg</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20060221</creationdate><title>The HIV Lipidome: A Raft with an Unusual Composition</title><author>Brügger, Britta ; Glass, Bärbel ; Haberkant, Per ; Leibrecht, Iris ; Wieland, Felix T. ; Kräusslich, Hans-Georg</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-a197ba2a83f9d6d4948dc2f4bae3adf8ec24c767cb3786f3ecdb4bf6b2b21de73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Biological Sciences</topic><topic>Cell membranes</topic><topic>Cells, Cultured</topic><topic>Cellular biology</topic><topic>Chemical composition</topic><topic>Cholesterols</topic><topic>HIV</topic><topic>HIV 1</topic><topic>HIV-1 - chemistry</topic><topic>HIV-1 - pathogenicity</topic><topic>Human immunodeficiency virus</topic><topic>Humans</topic><topic>Lipids</topic><topic>Mass Spectrometry</topic><topic>Membrane Microdomains - chemistry</topic><topic>Membranes</topic><topic>P branes</topic><topic>Phospholipids - analysis</topic><topic>Rafts</topic><topic>Sphingolipids</topic><topic>Sphingolipids - analysis</topic><topic>Sphingolipids - biosynthesis</topic><topic>T-Lymphocytes - virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brügger, Britta</creatorcontrib><creatorcontrib>Glass, Bärbel</creatorcontrib><creatorcontrib>Haberkant, Per</creatorcontrib><creatorcontrib>Leibrecht, Iris</creatorcontrib><creatorcontrib>Wieland, Felix T.</creatorcontrib><creatorcontrib>Kräusslich, Hans-Georg</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brügger, Britta</au><au>Glass, Bärbel</au><au>Haberkant, Per</au><au>Leibrecht, Iris</au><au>Wieland, Felix T.</au><au>Kräusslich, Hans-Georg</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The HIV Lipidome: A Raft with an Unusual Composition</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2006-02-21</date><risdate>2006</risdate><volume>103</volume><issue>8</issue><spage>2641</spage><epage>2646</epage><pages>2641-2646</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The lipids of enveloped viruses play critical roles in viral morphogenesis and infectivity. They are derived from the host membranes from which virus budding occurs, but the precise lipid composition has not been determined for any virus. Employing mass spectrometry, this study provides a quantitative analysis of the lipid constituents of HIV and a comprehensive comparison with its host membranes. Both a substantial enrichment of the unusual sphingolipid dihydrosphingomyelin and a loss of viral infectivity upon inhibition of sphingolipid biosynthesis in host cells are reported, establishing a critical role for this lipid class in the HIV replication cycle. Intriguingly, the overall lipid composition of native HIV membranes resembles detergent-resistant membrane microdomains and is strikingly different from that of host cell membranes. With this composition, the HIV lipidome provides strong evidence for the existence of lipid rafts in living cells.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>16481622</pmid><doi>10.1073/pnas.0511136103</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 2006-02, Vol.103 (8), p.2641-2646 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1413831 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Biological Sciences Cell membranes Cells, Cultured Cellular biology Chemical composition Cholesterols HIV HIV 1 HIV-1 - chemistry HIV-1 - pathogenicity Human immunodeficiency virus Humans Lipids Mass Spectrometry Membrane Microdomains - chemistry Membranes P branes Phospholipids - analysis Rafts Sphingolipids Sphingolipids - analysis Sphingolipids - biosynthesis T-Lymphocytes - virology Viruses |
| title | The HIV Lipidome: A Raft with an Unusual Composition |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-12T04%3A04%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20HIV%20Lipidome:%20A%20Raft%20with%20an%20Unusual%20Composition&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Br%C3%BCgger,%20Britta&rft.date=2006-02-21&rft.volume=103&rft.issue=8&rft.spage=2641&rft.epage=2646&rft.pages=2641-2646&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.0511136103&rft_dat=%3Cjstor_pubme%3E30049466%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=201420397&rft_id=info:pmid/16481622&rft_jstor_id=30049466&rfr_iscdi=true |