TRPC3 properties of a native constitutively active Ca2+-permeable cation channel in rabbit ear artery myocytes
Previously we have described a constitutively active, Ca 2 + -permeable, non-selective cation channel in freshly dispersed rabbit ear artery myocytes which has similar properties to some of the canonical transient receptor potential (TRPC) channel proteins. In the present work we have compared the p...
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| Veröffentlicht in: | The Journal of physiology 2006-03, Vol.571 (2), p.361-369 |
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| creator | Albert, A. P. Pucovský, V. Prestwich, S. A. Large, W. A. |
| description | Previously we have described a constitutively active, Ca 2 + -permeable, non-selective cation channel in freshly dispersed rabbit ear artery myocytes which has similar properties to some
of the canonical transient receptor potential (TRPC) channel proteins. In the present work we have compared the properties
of constitutive channel activity with known properties of TRPC proteins by investigating the effect of selective anti-TRPC
antibodies and pharmacological agents on whole-cell and single cation channel activity. Bath application of anti-TRPC3 antibodies
markedly reduced channel activity in inside-out patches and also produced a pronounced reduction of both current amplitude
and variance of constitutively active whole-cell cation currents whereas anti-TRPC1/4/5/6/7 antibodies had no effect on channel
activity. In the presence of antigenic peptide, anti-TRPC3 antibodies had no effect on whole-cell or single cation channel
activity. Bath application of flufenamic acid, Gd 3 + , La 3 + and Ca 2 + inhibited spontaneous channel activity in outside-out patches with IC 50 values of 6.8 μ m , 25 n m , 1.5 μ m and 0.124 m m , respectively, which are similar values to those against TRPC3 proteins. Immunocytochemical studies combined with confocal
microscopy showed expression of TRPC3 proteins in ear artery myocytes, and these were predominately distributed at, or close
to, the plasma membrane. These data provide strong evidence that native constitutively active cation channels in rabbit ear
artery myocytes have similar properties to TRPC3 channel proteins and indicate that these proteins may have an important role
in mediating this conductance. |
| doi_str_mv | 10.1113/jphysiol.2005.102780 |
| format | Article |
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of the canonical transient receptor potential (TRPC) channel proteins. In the present work we have compared the properties
of constitutive channel activity with known properties of TRPC proteins by investigating the effect of selective anti-TRPC
antibodies and pharmacological agents on whole-cell and single cation channel activity. Bath application of anti-TRPC3 antibodies
markedly reduced channel activity in inside-out patches and also produced a pronounced reduction of both current amplitude
and variance of constitutively active whole-cell cation currents whereas anti-TRPC1/4/5/6/7 antibodies had no effect on channel
activity. In the presence of antigenic peptide, anti-TRPC3 antibodies had no effect on whole-cell or single cation channel
activity. Bath application of flufenamic acid, Gd 3 + , La 3 + and Ca 2 + inhibited spontaneous channel activity in outside-out patches with IC 50 values of 6.8 μ m , 25 n m , 1.5 μ m and 0.124 m m , respectively, which are similar values to those against TRPC3 proteins. Immunocytochemical studies combined with confocal
microscopy showed expression of TRPC3 proteins in ear artery myocytes, and these were predominately distributed at, or close
to, the plasma membrane. These data provide strong evidence that native constitutively active cation channels in rabbit ear
artery myocytes have similar properties to TRPC3 channel proteins and indicate that these proteins may have an important role
in mediating this conductance.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.2005.102780</identifier><identifier>PMID: 16396924</identifier><language>eng</language><publisher>9600 Garsington Road , Oxford , OX4 2DQ , UK: The Physiological Society</publisher><subject>Animals ; Arteries - physiology ; Calcium Channels - metabolism ; Calcium Signaling ; Cations ; Cellular ; Ear - physiology ; Flufenamic Acid ; Immunohistochemistry ; Ion Channel Gating - physiology ; Ion Channels - physiology ; Membrane Potentials - physiology ; Microscopy, Confocal ; Myocytes, Smooth Muscle - metabolism ; Myocytes, Smooth Muscle - physiology ; Rabbits ; TRPC Cation Channels - metabolism ; TRPC Cation Channels - physiology</subject><ispartof>The Journal of physiology, 2006-03, Vol.571 (2), p.361-369</ispartof><rights>2006 The Journal of Physiology © 2006 The Physiological Society</rights><rights>2006 The Authors. Journal compilation © 2006 The Physiological Society 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1413579/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1413579/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,1427,27901,27902,45550,45551,46384,46808,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16396924$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Albert, A. P.</creatorcontrib><creatorcontrib>Pucovský, V.</creatorcontrib><creatorcontrib>Prestwich, S. A.</creatorcontrib><creatorcontrib>Large, W. A.</creatorcontrib><title>TRPC3 properties of a native constitutively active Ca2+-permeable cation channel in rabbit ear artery myocytes</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>Previously we have described a constitutively active, Ca 2 + -permeable, non-selective cation channel in freshly dispersed rabbit ear artery myocytes which has similar properties to some
of the canonical transient receptor potential (TRPC) channel proteins. In the present work we have compared the properties
of constitutive channel activity with known properties of TRPC proteins by investigating the effect of selective anti-TRPC
antibodies and pharmacological agents on whole-cell and single cation channel activity. Bath application of anti-TRPC3 antibodies
markedly reduced channel activity in inside-out patches and also produced a pronounced reduction of both current amplitude
and variance of constitutively active whole-cell cation currents whereas anti-TRPC1/4/5/6/7 antibodies had no effect on channel
activity. In the presence of antigenic peptide, anti-TRPC3 antibodies had no effect on whole-cell or single cation channel
activity. Bath application of flufenamic acid, Gd 3 + , La 3 + and Ca 2 + inhibited spontaneous channel activity in outside-out patches with IC 50 values of 6.8 μ m , 25 n m , 1.5 μ m and 0.124 m m , respectively, which are similar values to those against TRPC3 proteins. Immunocytochemical studies combined with confocal
microscopy showed expression of TRPC3 proteins in ear artery myocytes, and these were predominately distributed at, or close
to, the plasma membrane. These data provide strong evidence that native constitutively active cation channels in rabbit ear
artery myocytes have similar properties to TRPC3 channel proteins and indicate that these proteins may have an important role
in mediating this conductance.</description><subject>Animals</subject><subject>Arteries - physiology</subject><subject>Calcium Channels - metabolism</subject><subject>Calcium Signaling</subject><subject>Cations</subject><subject>Cellular</subject><subject>Ear - physiology</subject><subject>Flufenamic Acid</subject><subject>Immunohistochemistry</subject><subject>Ion Channel Gating - physiology</subject><subject>Ion Channels - physiology</subject><subject>Membrane Potentials - physiology</subject><subject>Microscopy, Confocal</subject><subject>Myocytes, Smooth Muscle - metabolism</subject><subject>Myocytes, Smooth Muscle - physiology</subject><subject>Rabbits</subject><subject>TRPC Cation Channels - metabolism</subject><subject>TRPC Cation Channels - physiology</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>EIF</sourceid><recordid>eNpVkU1v1DAQhi0EotuFf4CQT3BAWTx24sQXpGoFBVSJCi1ny3FmG1eJvdjeVvn3ZEnLx2k0M8-8M5qXkFfANgAg3t8e-im5MGw4Y9UGGK8b9oSsoJSqqGslnpIVY5wXoq7gjJyndMsYCKbUc3IGUiipeLkifvf9eivoIYYDxuww0bCnhnqT3R1SG3zKLh9PyTBRY39Xt4a_K2Z8RNMOMzSzwVPbG-9xoM7TaNrWZYomUhMzxomOU7BTxvSCPNubIeHLh7gmPz593G0_F1ffLr9sL66KXjRNVczHSWtnFaG6EhrGO9V13R4agw20dc3KfVOqitUVByNaW8tOltxiCYpLbrlYkw-L7uHYjthZ9DmaQR-iG02cdDBO_9_xrtc34U5DCaKav7cmbx4EYvh5xJT16JLFYTAewzFpOR8hgFcz-PrfTX9WPL54BtQC3LsBp799pk826kcb9clGvdiod1-vQciT-Ntltnc3_b2LqBc6BeswT7qqQXMtJIhf0uuiOA</recordid><startdate>200603</startdate><enddate>200603</enddate><creator>Albert, A. P.</creator><creator>Pucovský, V.</creator><creator>Prestwich, S. A.</creator><creator>Large, W. A.</creator><general>The Physiological Society</general><general>Blackwell Science Ltd</general><general>Blackwell Science Inc</general><scope>24P</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200603</creationdate><title>TRPC3 properties of a native constitutively active Ca2+-permeable cation channel in rabbit ear artery myocytes</title><author>Albert, A. P. ; Pucovský, V. ; Prestwich, S. A. ; Large, W. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h3885-9696ccabb39d41802d9dddf18ae81b7704f849507521a3bc76d642ce419262c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Arteries - physiology</topic><topic>Calcium Channels - metabolism</topic><topic>Calcium Signaling</topic><topic>Cations</topic><topic>Cellular</topic><topic>Ear - physiology</topic><topic>Flufenamic Acid</topic><topic>Immunohistochemistry</topic><topic>Ion Channel Gating - physiology</topic><topic>Ion Channels - physiology</topic><topic>Membrane Potentials - physiology</topic><topic>Microscopy, Confocal</topic><topic>Myocytes, Smooth Muscle - metabolism</topic><topic>Myocytes, Smooth Muscle - physiology</topic><topic>Rabbits</topic><topic>TRPC Cation Channels - metabolism</topic><topic>TRPC Cation Channels - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Albert, A. P.</creatorcontrib><creatorcontrib>Pucovský, V.</creatorcontrib><creatorcontrib>Prestwich, S. A.</creatorcontrib><creatorcontrib>Large, W. A.</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Albert, A. P.</au><au>Pucovský, V.</au><au>Prestwich, S. A.</au><au>Large, W. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TRPC3 properties of a native constitutively active Ca2+-permeable cation channel in rabbit ear artery myocytes</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>2006-03</date><risdate>2006</risdate><volume>571</volume><issue>2</issue><spage>361</spage><epage>369</epage><pages>361-369</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><abstract>Previously we have described a constitutively active, Ca 2 + -permeable, non-selective cation channel in freshly dispersed rabbit ear artery myocytes which has similar properties to some
of the canonical transient receptor potential (TRPC) channel proteins. In the present work we have compared the properties
of constitutive channel activity with known properties of TRPC proteins by investigating the effect of selective anti-TRPC
antibodies and pharmacological agents on whole-cell and single cation channel activity. Bath application of anti-TRPC3 antibodies
markedly reduced channel activity in inside-out patches and also produced a pronounced reduction of both current amplitude
and variance of constitutively active whole-cell cation currents whereas anti-TRPC1/4/5/6/7 antibodies had no effect on channel
activity. In the presence of antigenic peptide, anti-TRPC3 antibodies had no effect on whole-cell or single cation channel
activity. Bath application of flufenamic acid, Gd 3 + , La 3 + and Ca 2 + inhibited spontaneous channel activity in outside-out patches with IC 50 values of 6.8 μ m , 25 n m , 1.5 μ m and 0.124 m m , respectively, which are similar values to those against TRPC3 proteins. Immunocytochemical studies combined with confocal
microscopy showed expression of TRPC3 proteins in ear artery myocytes, and these were predominately distributed at, or close
to, the plasma membrane. These data provide strong evidence that native constitutively active cation channels in rabbit ear
artery myocytes have similar properties to TRPC3 channel proteins and indicate that these proteins may have an important role
in mediating this conductance.</abstract><cop>9600 Garsington Road , Oxford , OX4 2DQ , UK</cop><pub>The Physiological Society</pub><pmid>16396924</pmid><doi>10.1113/jphysiol.2005.102780</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Arteries - physiology Calcium Channels - metabolism Calcium Signaling Cations Cellular Ear - physiology Flufenamic Acid Immunohistochemistry Ion Channel Gating - physiology Ion Channels - physiology Membrane Potentials - physiology Microscopy, Confocal Myocytes, Smooth Muscle - metabolism Myocytes, Smooth Muscle - physiology Rabbits TRPC Cation Channels - metabolism TRPC Cation Channels - physiology |
| title | TRPC3 properties of a native constitutively active Ca2+-permeable cation channel in rabbit ear artery myocytes |
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