Method of preparing isolated colonic epithelial cells (colonocytes) for metabolic studies

Suspensions of isolated colonic epithelial cells (colonocytes) have been obtained from rats by incubating everted lengths of colon with EDTA at 37 degrees C in Krebs-Henseleit saline from which calcium was omitted and containing 0.25% (w/v) bovine serum albumin. Measurements of oxygen consumption an...

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Veröffentlicht in:	Gut 1979-06, Vol.20 (6), p.484-488
Hauptverfasser:	Roediger, W E, Truelove, S C
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Separation - methods Cell Survival Colon - cytology Colon - metabolism Epithelial Cells Epithelium - metabolism Glycolysis Humans In Vitro Techniques Male Oxygen Consumption Rats
Online-Zugang:	Volltext
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