Fowl antibody. X. The purification and properties of an antibody to the 2,4-dinitrophenyl group

A preparation of fowl antibody to the 2,4-dinitrophenyl group (DNP), made from antisera to DNP-bovine γ-globulin by dissociation of a specific precipitate, was more than 90 per cent precipitable by DNP-ovalbumin. This antibody with an approximate molecular weight of 165,000 and the immunoelectrophor...

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Veröffentlicht in:	Immunology 1968-01, Vol.14 (1), p.61-67
1. Verfasser:	Orlans, E
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Sprache:	eng
Schlagworte:	Amino Acids - analysis Animals Antibodies - analysis Chickens - immunology Complement System Proteins Dinitrophenols Female gamma-Globulins Haptens Humans Immunoelectrophoresis Ovalbumin Passive Cutaneous Anaphylaxis Serum Albumin Serum Albumin, Bovine Ultracentrifugation
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creator	Orlans, E
description	A preparation of fowl antibody to the 2,4-dinitrophenyl group (DNP), made from antisera to DNP-bovine γ-globulin by dissociation of a specific precipitate, was more than 90 per cent precipitable by DNP-ovalbumin. This antibody with an approximate molecular weight of 165,000 and the immunoelectrophoretic properties of γG-globulin, precipitated with antigen only in 8 per cent NaCl. It fixed fowl complement, but did not agglutinate tanned fowl red cells coated with DNP-protein. It had no skin sensitizing activity. Its amino acid composition was significantly different from that of mammalian antibodies. Some of the properties of another DNP-specific antibody that appeared later in immunization are also described.
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The purification and properties of an antibody to the 2,4-dinitrophenyl group</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Orlans, E</creator><creatorcontrib>Orlans, E</creatorcontrib><description>A preparation of fowl antibody to the 2,4-dinitrophenyl group (DNP), made from antisera to DNP-bovine γ-globulin by dissociation of a specific precipitate, was more than 90 per cent precipitable by DNP-ovalbumin. This antibody with an approximate molecular weight of 165,000 and the immunoelectrophoretic properties of γG-globulin, precipitated with antigen only in 8 per cent NaCl. It fixed fowl complement, but did not agglutinate tanned fowl red cells coated with DNP-protein. It had no skin sensitizing activity. Its amino acid composition was significantly different from that of mammalian antibodies. Some of the properties of another DNP-specific antibody that appeared later in immunization are also described.</description><identifier>ISSN: 0019-2805</identifier><identifier>EISSN: 1365-2567</identifier><identifier>PMID: 4169726</identifier><language>eng</language><publisher>England</publisher><subject>Amino Acids - analysis ; Animals ; Antibodies - analysis ; Chickens - immunology ; Complement System Proteins ; Dinitrophenols ; Female ; gamma-Globulins ; Haptens ; Humans ; Immunoelectrophoresis ; Ovalbumin ; Passive Cutaneous Anaphylaxis ; Serum Albumin ; Serum Albumin, Bovine ; Ultracentrifugation</subject><ispartof>Immunology, 1968-01, Vol.14 (1), p.61-67</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1409260/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1409260/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4169726$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Orlans, E</creatorcontrib><title>Fowl antibody. X. The purification and properties of an antibody to the 2,4-dinitrophenyl group</title><title>Immunology</title><addtitle>Immunology</addtitle><description>A preparation of fowl antibody to the 2,4-dinitrophenyl group (DNP), made from antisera to DNP-bovine γ-globulin by dissociation of a specific precipitate, was more than 90 per cent precipitable by DNP-ovalbumin. This antibody with an approximate molecular weight of 165,000 and the immunoelectrophoretic properties of γG-globulin, precipitated with antigen only in 8 per cent NaCl. It fixed fowl complement, but did not agglutinate tanned fowl red cells coated with DNP-protein. It had no skin sensitizing activity. Its amino acid composition was significantly different from that of mammalian antibodies. Some of the properties of another DNP-specific antibody that appeared later in immunization are also described.</description><subject>Amino Acids - analysis</subject><subject>Animals</subject><subject>Antibodies - analysis</subject><subject>Chickens - immunology</subject><subject>Complement System Proteins</subject><subject>Dinitrophenols</subject><subject>Female</subject><subject>gamma-Globulins</subject><subject>Haptens</subject><subject>Humans</subject><subject>Immunoelectrophoresis</subject><subject>Ovalbumin</subject><subject>Passive Cutaneous Anaphylaxis</subject><subject>Serum Albumin</subject><subject>Serum Albumin, Bovine</subject><subject>Ultracentrifugation</subject><issn>0019-2805</issn><issn>1365-2567</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1968</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM1KAzEUhYMotVYfQcjKlVPyn8xGkGJVENxUcBcyM0kbmU5iJqP07R21FF1d7j3nfAfuEZhiKnhBuJDHYIoQLguiED8FZ33_Nq4UcT4BE4ZFKYmYAr0Mny00XfZVaHZz-DqHq42FcUje-dpkH7pRbWBMIdqUve1hcOPlEIE5wDwmyDUrGt_5PBo3ttu1cJ3CEM_BiTNtby_2cwZelnerxUPx9Hz_uLh9KiKWIhd1XQrpJKKlq7kjTDDHVS0axynFsiqVUaSx2AlUSsGRqrBCpDFOMMuQsojOwM0vNw7V1ja17XIyrY7Jb03a6WC8_q90fqPX4UNjhkoivgFXe0AK74Pts976vrZtazobhl4rJgWVP8bLv02Hiv1L6RcctXRy</recordid><startdate>196801</startdate><enddate>196801</enddate><creator>Orlans, E</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>196801</creationdate><title>Fowl antibody. X. The purification and properties of an antibody to the 2,4-dinitrophenyl group</title><author>Orlans, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p176t-cc967f7039fc5f2464f58c6df53317b98a82de1f60976508b1802daf64e408e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1968</creationdate><topic>Amino Acids - analysis</topic><topic>Animals</topic><topic>Antibodies - analysis</topic><topic>Chickens - immunology</topic><topic>Complement System Proteins</topic><topic>Dinitrophenols</topic><topic>Female</topic><topic>gamma-Globulins</topic><topic>Haptens</topic><topic>Humans</topic><topic>Immunoelectrophoresis</topic><topic>Ovalbumin</topic><topic>Passive Cutaneous Anaphylaxis</topic><topic>Serum Albumin</topic><topic>Serum Albumin, Bovine</topic><topic>Ultracentrifugation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Orlans, E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Orlans, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fowl antibody. X. The purification and properties of an antibody to the 2,4-dinitrophenyl group</atitle><jtitle>Immunology</jtitle><addtitle>Immunology</addtitle><date>1968-01</date><risdate>1968</risdate><volume>14</volume><issue>1</issue><spage>61</spage><epage>67</epage><pages>61-67</pages><issn>0019-2805</issn><eissn>1365-2567</eissn><abstract>A preparation of fowl antibody to the 2,4-dinitrophenyl group (DNP), made from antisera to DNP-bovine γ-globulin by dissociation of a specific precipitate, was more than 90 per cent precipitable by DNP-ovalbumin. This antibody with an approximate molecular weight of 165,000 and the immunoelectrophoretic properties of γG-globulin, precipitated with antigen only in 8 per cent NaCl. It fixed fowl complement, but did not agglutinate tanned fowl red cells coated with DNP-protein. It had no skin sensitizing activity. Its amino acid composition was significantly different from that of mammalian antibodies. Some of the properties of another DNP-specific antibody that appeared later in immunization are also described.</abstract><cop>England</cop><pmid>4169726</pmid><tpages>7</tpages></addata></record>
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subjects	Amino Acids - analysis Animals Antibodies - analysis Chickens - immunology Complement System Proteins Dinitrophenols Female gamma-Globulins Haptens Humans Immunoelectrophoresis Ovalbumin Passive Cutaneous Anaphylaxis Serum Albumin Serum Albumin, Bovine Ultracentrifugation
title	Fowl antibody. X. The purification and properties of an antibody to the 2,4-dinitrophenyl group
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