Analysis of site-specific protein-RNA cross-links in isolated RNP complexes, combining affinity selection and mass spectrometry

An important aspect of the assembly of RNPs, and in particular of spliceosomes, is the succession of proteins bound to any given site on the RNA. Protein-RNA cross-linking is a well-established technique for investigating this, but the identification of a cross-linked protein has so far relied upon...

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Veröffentlicht in:	RNA (Cambridge) 2003-12, Vol.9 (12), p.1542-1551
Hauptverfasser:	Rhode, Britta M, Hartmuth, Klaus, Urlaub, Henning, Luhrmann, Reinhard
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biotin - chemistry DNA Primers Heterogeneous-nuclear ribonucleoproteins Mass Spectrometry - methods Method RNA - chemistry RNA-Binding Proteins - chemistry Streptavidin - chemistry
Online-Zugang:	Volltext
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