Photothermal Nanotherapeutics and Nanodiagnostics for Selective Killing of Bacteria Targeted with Gold Nanoparticles
We describe a new method for selective laser killing of bacteria targeted with light-absorbing gold nanoparticles conjugated with specific antibodies. The multifunctional photothermal (PT) microscope/spectrometer provides a real-time assessment of this new therapeutic intervention. In this integrate...
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| Veröffentlicht in: | Biophysical journal 2006-01, Vol.90 (2), p.619-627 |
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| creator | Zharov, Vladimir P. Mercer, Kelly E. Galitovskaya, Elena N. Smeltzer, Mark S. |
| description | We describe a new method for selective laser killing of bacteria targeted with light-absorbing gold nanoparticles conjugated with specific antibodies. The multifunctional photothermal (PT) microscope/spectrometer provides a real-time assessment of this new therapeutic intervention. In this integrated system, strong laser-induced overheating effects accompanied by the bubble-formation phenomena around clustered gold nanoparticles are the main cause of bacterial damage. PT imaging and time-resolved monitoring of the integrated PT responses assessed these effects. Specifically, we used this technology for selective killing of the Gram-positive bacterium
Staphylococcus aureus by targeting the bacterial surface using 10-, 20-, and 40-nm gold particles conjugated with anti-protein A antibodies. Labeled bacteria were irradiated with focused laser pulses (420–570
nm, 12
ns, 0.1–5
J/cm
2, 100 pulses), and laser-induced bacterial damage observed at different laser fluences and nanoparticle sizes was verified by optical transmission, electron microscopy, and conventional viability testing. |
| doi_str_mv | 10.1529/biophysj.105.061895 |
| format | Article |
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Staphylococcus aureus by targeting the bacterial surface using 10-, 20-, and 40-nm gold particles conjugated with anti-protein A antibodies. Labeled bacteria were irradiated with focused laser pulses (420–570
nm, 12
ns, 0.1–5
J/cm
2, 100 pulses), and laser-induced bacterial damage observed at different laser fluences and nanoparticle sizes was verified by optical transmission, electron microscopy, and conventional viability testing.</description><identifier>ISSN: 0006-3495</identifier><identifier>EISSN: 1542-0086</identifier><identifier>DOI: 10.1529/biophysj.105.061895</identifier><identifier>PMID: 16239330</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Effects ; Gold ; Gold - chemistry ; Gram-positive bacteria ; Hot Temperature ; Immunoglobulins ; Lasers ; Low-Level Light Therapy - methods ; Medical imaging ; Medical research ; Microscopy ; Microscopy, Electron ; Microscopy, Electron, Transmission ; Microscopy, Phase-Contrast ; Nanoparticles ; Nanostructures - chemistry ; Nanotechnology - methods ; Phototherapy - methods ; Protein Transport ; Proteins ; Spectrophotometry ; Spectroscopy, Imaging, Other Techniques ; Spectrum Analysis, Raman ; Staphylococcus aureus - metabolism ; Staphylococcus aureus - radiation effects ; Surface Plasmon Resonance ; Temperature ; Time Factors</subject><ispartof>Biophysical journal, 2006-01, Vol.90 (2), p.619-627</ispartof><rights>2006 The Biophysical Society</rights><rights>Copyright Biophysical Society Jan 15, 2006</rights><rights>Copyright © 2006, Biophysical Society 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-f9cc6e080d9b601509b8adfc3b6b8717cadfdea0345b816e85021ba5ba9119bc3</citedby><cites>FETCH-LOGICAL-c484t-f9cc6e080d9b601509b8adfc3b6b8717cadfdea0345b816e85021ba5ba9119bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1367066/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1529/biophysj.105.061895$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,3550,27924,27925,45995,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16239330$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zharov, Vladimir P.</creatorcontrib><creatorcontrib>Mercer, Kelly E.</creatorcontrib><creatorcontrib>Galitovskaya, Elena N.</creatorcontrib><creatorcontrib>Smeltzer, Mark S.</creatorcontrib><title>Photothermal Nanotherapeutics and Nanodiagnostics for Selective Killing of Bacteria Targeted with Gold Nanoparticles</title><title>Biophysical journal</title><addtitle>Biophys J</addtitle><description>We describe a new method for selective laser killing of bacteria targeted with light-absorbing gold nanoparticles conjugated with specific antibodies. The multifunctional photothermal (PT) microscope/spectrometer provides a real-time assessment of this new therapeutic intervention. In this integrated system, strong laser-induced overheating effects accompanied by the bubble-formation phenomena around clustered gold nanoparticles are the main cause of bacterial damage. PT imaging and time-resolved monitoring of the integrated PT responses assessed these effects. Specifically, we used this technology for selective killing of the Gram-positive bacterium
Staphylococcus aureus by targeting the bacterial surface using 10-, 20-, and 40-nm gold particles conjugated with anti-protein A antibodies. Labeled bacteria were irradiated with focused laser pulses (420–570
nm, 12
ns, 0.1–5
J/cm
2, 100 pulses), and laser-induced bacterial damage observed at different laser fluences and nanoparticle sizes was verified by optical transmission, electron microscopy, and conventional viability testing.</description><subject>Effects</subject><subject>Gold</subject><subject>Gold - chemistry</subject><subject>Gram-positive bacteria</subject><subject>Hot Temperature</subject><subject>Immunoglobulins</subject><subject>Lasers</subject><subject>Low-Level Light Therapy - methods</subject><subject>Medical imaging</subject><subject>Medical research</subject><subject>Microscopy</subject><subject>Microscopy, Electron</subject><subject>Microscopy, Electron, Transmission</subject><subject>Microscopy, Phase-Contrast</subject><subject>Nanoparticles</subject><subject>Nanostructures - chemistry</subject><subject>Nanotechnology - methods</subject><subject>Phototherapy - methods</subject><subject>Protein Transport</subject><subject>Proteins</subject><subject>Spectrophotometry</subject><subject>Spectroscopy, Imaging, Other Techniques</subject><subject>Spectrum Analysis, Raman</subject><subject>Staphylococcus aureus - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biophysical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zharov, Vladimir P.</au><au>Mercer, Kelly E.</au><au>Galitovskaya, Elena N.</au><au>Smeltzer, Mark S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photothermal Nanotherapeutics and Nanodiagnostics for Selective Killing of Bacteria Targeted with Gold Nanoparticles</atitle><jtitle>Biophysical journal</jtitle><addtitle>Biophys J</addtitle><date>2006-01-01</date><risdate>2006</risdate><volume>90</volume><issue>2</issue><spage>619</spage><epage>627</epage><pages>619-627</pages><issn>0006-3495</issn><eissn>1542-0086</eissn><abstract>We describe a new method for selective laser killing of bacteria targeted with light-absorbing gold nanoparticles conjugated with specific antibodies. The multifunctional photothermal (PT) microscope/spectrometer provides a real-time assessment of this new therapeutic intervention. In this integrated system, strong laser-induced overheating effects accompanied by the bubble-formation phenomena around clustered gold nanoparticles are the main cause of bacterial damage. PT imaging and time-resolved monitoring of the integrated PT responses assessed these effects. Specifically, we used this technology for selective killing of the Gram-positive bacterium
Staphylococcus aureus by targeting the bacterial surface using 10-, 20-, and 40-nm gold particles conjugated with anti-protein A antibodies. Labeled bacteria were irradiated with focused laser pulses (420–570
nm, 12
ns, 0.1–5
J/cm
2, 100 pulses), and laser-induced bacterial damage observed at different laser fluences and nanoparticle sizes was verified by optical transmission, electron microscopy, and conventional viability testing.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16239330</pmid><doi>10.1529/biophysj.105.061895</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biophysical journal, 2006-01, Vol.90 (2), p.619-627 |
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| language | eng |
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| source | MEDLINE; Cell Press Free Archives; Access via ScienceDirect (Elsevier); PubMed Central; EZB Electronic Journals Library |
| subjects | Effects Gold Gold - chemistry Gram-positive bacteria Hot Temperature Immunoglobulins Lasers Low-Level Light Therapy - methods Medical imaging Medical research Microscopy Microscopy, Electron Microscopy, Electron, Transmission Microscopy, Phase-Contrast Nanoparticles Nanostructures - chemistry Nanotechnology - methods Phototherapy - methods Protein Transport Proteins Spectrophotometry Spectroscopy, Imaging, Other Techniques Spectrum Analysis, Raman Staphylococcus aureus - metabolism Staphylococcus aureus - radiation effects Surface Plasmon Resonance Temperature Time Factors |
| title | Photothermal Nanotherapeutics and Nanodiagnostics for Selective Killing of Bacteria Targeted with Gold Nanoparticles |
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