Half-Sarcomere Dynamics in Myofibrils during Activation and Relaxation Studied by Tracking Fluorescent Markers

To study the dynamics of individual half-sarcomeres in striated muscle contraction, myofibrils prepared from rabbit psoas muscle and left ventricles of guinea pig were immunostained with two conjugated antibody complexes consisting of a primary antibody against either α-actinin or myomesin and a sec...

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Veröffentlicht in:	Biophysical journal 2006-01, Vol.90 (2), p.514-530
Hauptverfasser:	Telley, Ivo A., Denoth, Jachen, Stüssi, Edgar, Pfitzer, Gabriele, Stehle, Robert
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinin - chemistry Algorithms Animals Calcium - metabolism Connectin Dithiothreitol - pharmacology Fluorescence Fluorescent Dyes - pharmacology Guinea Pigs Heart Ventricles - metabolism Immunoglobulins Kinetics Microscopy, Fluorescence Models, Biological Muscle and Contractility Muscle Contraction Muscle Proteins - chemistry Muscles - metabolism Muscular system Myocardium - metabolism Myofibrils - chemistry Oscillometry Rabbits Sarcomeres - chemistry Temperature Time Factors
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