Main Phase Transitions in Supported Lipid Single-Bilayer
We have studied the phase transitions of a phospholipidic single-bilayer supported on a mica substrate by real-time temperature-controlled atomic force microscopy. We show the existence of two phase transitions in this bilayer that we attribute to two gel (L β )/fluid (L α ) transitions, correspondi...
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Veröffentlicht in: | Biophysical journal 2005-08, Vol.89 (2), p.1094-1101 |
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Hauptverfasser: | , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | We have studied the phase transitions of a phospholipidic single-bilayer supported on a mica substrate by real-time temperature-controlled atomic force microscopy. We show the existence of two phase transitions in this bilayer that we attribute to two gel (L
β
)/fluid (L
α
) transitions, corresponding to the independent melting of each leaflet of the bilayer. The ratio of each phase with temperature and the large broadening of the transitions’ widths have been interpreted through a basic thermodynamic framework in which the surface tension varies during the transitions. The experimental data can be fit with such a model using known thermodynamic parameters. |
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ISSN: | 0006-3495 1542-0086 |
DOI: | 10.1529/biophysj.105.062463 |