BTA, a novel reagent for DNA attachment on glass and efficient generation of solid-phase amplified DNA colonies

The tricarboxylate reagent benzene-1,3,5-triacetic acid (BTA) was used to attach 5′-aminated DNA primers and templates on an aminosilanized glass surface for subsequent generation of DNA colonies by in situ solid-phase amplification. We have characterized the derivatized surfaces for the chemical at...

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Veröffentlicht in:	Nucleic acids research 2006-01, Vol.34 (3), p.e22-e22
Hauptverfasser:	Fedurco, Milan, Romieu, Anthony, Williams, Scott, Lawrence, Isabelle, Turcatti, Gerardo
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetates - chemistry Cross-Linking Reagents - chemistry Deoxyribonucleases, Type II Site-Specific - metabolism DNA - analysis DNA - biosynthesis DNA - chemistry DNA Primers Genomics - methods Glass - chemistry Methods Online Microfluidic Analytical Techniques Oligonucleotides - chemistry Polymerase Chain Reaction - methods Temperature Templates, Genetic
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