Importance of Heat-Labile Enterotoxin in Colonization of the Adult Mouse Small Intestine by Human Enterotoxigenic Escherichia coli Strains
Enterotoxigenic Escherichia coli (ETEC) infections are a significant cause of diarrheal disease and infant mortality in developing countries. Studies of ETEC pathogenesis relevant to vaccine development have been greatly hampered by the lack of a suitable small-animal model of infection with human E...
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| Veröffentlicht in: | Infection and Immunity 2006-02, Vol.74 (2), p.869-875 |
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| creator | Allen, Kenneth P Randolph, Mildred M Fleckenstein, James M |
| description | Enterotoxigenic Escherichia coli (ETEC) infections are a significant cause of diarrheal disease and infant mortality in developing countries. Studies of ETEC pathogenesis relevant to vaccine development have been greatly hampered by the lack of a suitable small-animal model of infection with human ETEC strains. Here, we demonstrate that adult immunocompetent outbred mice can be effectively colonized with the prototypical human ETEC H10407 strain (colonization factor antigen I; heat-labile and heat-stable enterotoxin positive) and that production of heat-labile holotoxin provides a significant advantage in colonization of the small intestine in this model. |
| doi_str_mv | 10.1128/IAI.74.2.869-875.2006 |
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Studies of ETEC pathogenesis relevant to vaccine development have been greatly hampered by the lack of a suitable small-animal model of infection with human ETEC strains. Here, we demonstrate that adult immunocompetent outbred mice can be effectively colonized with the prototypical human ETEC H10407 strain (colonization factor antigen I; heat-labile and heat-stable enterotoxin positive) and that production of heat-labile holotoxin provides a significant advantage in colonization of the small intestine in this model.</description><identifier>ISSN: 0019-9567</identifier><identifier>EISSN: 1098-5522</identifier><identifier>DOI: 10.1128/IAI.74.2.869-875.2006</identifier><identifier>PMID: 16428729</identifier><identifier>CODEN: INFIBR</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>adults ; Animals ; bacterial colonization ; Bacterial Infections ; Bacterial Toxins - metabolism ; Bacteriology ; Biological and medical sciences ; Child, Preschool ; Colony Count, Microbial ; Disease Models, Animal ; enterotoxins ; Enterotoxins - metabolism ; Escherichia coli ; Escherichia coli - classification ; Escherichia coli - growth & development ; Escherichia coli - pathogenicity ; Escherichia coli Infections - microbiology ; Escherichia coli Infections - pathology ; Escherichia coli Proteins - metabolism ; Escherichia infections ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Intestine, Small - microbiology ; Intestine, Small - pathology ; Mice ; Mice, Inbred ICR ; Microbiology ; Microscopy, Electron, Scanning ; Miscellaneous ; small intestine ; strains</subject><ispartof>Infection and Immunity, 2006-02, Vol.74 (2), p.869-875</ispartof><rights>2006 INIST-CNRS</rights><rights>Copyright © 2006, American Society for Microbiology 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-92fd6eac1eb5e0bf9ad1b25ffbab2b458db763f51713f7fe59867d555b008d873</citedby><cites>FETCH-LOGICAL-c472t-92fd6eac1eb5e0bf9ad1b25ffbab2b458db763f51713f7fe59867d555b008d873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1360293/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1360293/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17474823$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16428729$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Allen, Kenneth P</creatorcontrib><creatorcontrib>Randolph, Mildred M</creatorcontrib><creatorcontrib>Fleckenstein, James M</creatorcontrib><title>Importance of Heat-Labile Enterotoxin in Colonization of the Adult Mouse Small Intestine by Human Enterotoxigenic Escherichia coli Strains</title><title>Infection and Immunity</title><addtitle>Infect Immun</addtitle><description>Enterotoxigenic Escherichia coli (ETEC) infections are a significant cause of diarrheal disease and infant mortality in developing countries. Studies of ETEC pathogenesis relevant to vaccine development have been greatly hampered by the lack of a suitable small-animal model of infection with human ETEC strains. Here, we demonstrate that adult immunocompetent outbred mice can be effectively colonized with the prototypical human ETEC H10407 strain (colonization factor antigen I; heat-labile and heat-stable enterotoxin positive) and that production of heat-labile holotoxin provides a significant advantage in colonization of the small intestine in this model.</description><subject>adults</subject><subject>Animals</subject><subject>bacterial colonization</subject><subject>Bacterial Infections</subject><subject>Bacterial Toxins - metabolism</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Child, Preschool</subject><subject>Colony Count, Microbial</subject><subject>Disease Models, Animal</subject><subject>enterotoxins</subject><subject>Enterotoxins - metabolism</subject><subject>Escherichia coli</subject><subject>Escherichia coli - classification</subject><subject>Escherichia coli - growth & development</subject><subject>Escherichia coli - pathogenicity</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli Infections - pathology</subject><subject>Escherichia coli Proteins - metabolism</subject><subject>Escherichia infections</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Intestine, Small - microbiology</subject><subject>Intestine, Small - pathology</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Microbiology</subject><subject>Microscopy, Electron, Scanning</subject><subject>Miscellaneous</subject><subject>small intestine</subject><subject>strains</subject><issn>0019-9567</issn><issn>1098-5522</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkdGOEyEUhidG49bVR1C50bupcGYYmBuTpqm2SY0Xda8Jw0AHw0CF6er6CD61NK3uemVCQgjf-Q-HryheEjwnBPi7zWIzZ_Uc5rxpS87oHDBuHhUzglteUgrwuJhhTNqypQ27Kp6l9DUf67rmT4sr0tTAGbSz4tdmPIQ4Sa80CgattZzKreys02jlJx3DFH5Yj_JaBhe8_SknG_wJnQaNFv3RTehTOCaNdqN0Dm1yUZqs16i7Q-vjKP2DnL32VqFVUoOOVg1WIhWcRbspSuvT8-KJkS7pF5f9urj5sPqyXJfbzx83y8W2VDWDqWzB9I2WiuiOatyZVvakA2pMJzvoasr7jjWVoYSRyjCjacsb1lNKO4x5z1l1Xbw_5x6O3ah7pX3u78Qh2lHGOxGkFf_eeDuIfbgVpGowtFUOeHsJiOHbMY8rRpuUdk56nb9CMNzwFgD-C0J2BZienkTPoIohpajN39cQLE66RdYtWC1AZN0i6xYn3bnu1cNR7qsufjPw5gLIpKQzMYu26Z5jNas5nEZCZ26w--G7jVrINAqbv-JP04y8PiNGBiH3Mcfc7ACTChNMMQCufgOt4ctK</recordid><startdate>20060201</startdate><enddate>20060201</enddate><creator>Allen, Kenneth P</creator><creator>Randolph, Mildred M</creator><creator>Fleckenstein, James M</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20060201</creationdate><title>Importance of Heat-Labile Enterotoxin in Colonization of the Adult Mouse Small Intestine by Human Enterotoxigenic Escherichia coli Strains</title><author>Allen, Kenneth P ; Randolph, Mildred M ; Fleckenstein, James M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-92fd6eac1eb5e0bf9ad1b25ffbab2b458db763f51713f7fe59867d555b008d873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>adults</topic><topic>Animals</topic><topic>bacterial colonization</topic><topic>Bacterial Infections</topic><topic>Bacterial Toxins - metabolism</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Child, Preschool</topic><topic>Colony Count, Microbial</topic><topic>Disease Models, Animal</topic><topic>enterotoxins</topic><topic>Enterotoxins - metabolism</topic><topic>Escherichia coli</topic><topic>Escherichia coli - classification</topic><topic>Escherichia coli - growth & development</topic><topic>Escherichia coli - pathogenicity</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Escherichia coli Infections - pathology</topic><topic>Escherichia coli Proteins - metabolism</topic><topic>Escherichia infections</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Intestine, Small - microbiology</topic><topic>Intestine, Small - pathology</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>Microbiology</topic><topic>Microscopy, Electron, Scanning</topic><topic>Miscellaneous</topic><topic>small intestine</topic><topic>strains</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Allen, Kenneth P</creatorcontrib><creatorcontrib>Randolph, Mildred M</creatorcontrib><creatorcontrib>Fleckenstein, James M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Infection and Immunity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Allen, Kenneth P</au><au>Randolph, Mildred M</au><au>Fleckenstein, James M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Importance of Heat-Labile Enterotoxin in Colonization of the Adult Mouse Small Intestine by Human Enterotoxigenic Escherichia coli Strains</atitle><jtitle>Infection and Immunity</jtitle><addtitle>Infect Immun</addtitle><date>2006-02-01</date><risdate>2006</risdate><volume>74</volume><issue>2</issue><spage>869</spage><epage>875</epage><pages>869-875</pages><issn>0019-9567</issn><eissn>1098-5522</eissn><coden>INFIBR</coden><abstract>Enterotoxigenic Escherichia coli (ETEC) infections are a significant cause of diarrheal disease and infant mortality in developing countries. Studies of ETEC pathogenesis relevant to vaccine development have been greatly hampered by the lack of a suitable small-animal model of infection with human ETEC strains. Here, we demonstrate that adult immunocompetent outbred mice can be effectively colonized with the prototypical human ETEC H10407 strain (colonization factor antigen I; heat-labile and heat-stable enterotoxin positive) and that production of heat-labile holotoxin provides a significant advantage in colonization of the small intestine in this model.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>16428729</pmid><doi>10.1128/IAI.74.2.869-875.2006</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | American Society for Microbiology; MEDLINE; PMC (PubMed Central); Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | adults Animals bacterial colonization Bacterial Infections Bacterial Toxins - metabolism Bacteriology Biological and medical sciences Child, Preschool Colony Count, Microbial Disease Models, Animal enterotoxins Enterotoxins - metabolism Escherichia coli Escherichia coli - classification Escherichia coli - growth & development Escherichia coli - pathogenicity Escherichia coli Infections - microbiology Escherichia coli Infections - pathology Escherichia coli Proteins - metabolism Escherichia infections Female Fundamental and applied biological sciences. Psychology Humans Intestine, Small - microbiology Intestine, Small - pathology Mice Mice, Inbred ICR Microbiology Microscopy, Electron, Scanning Miscellaneous small intestine strains |
| title | Importance of Heat-Labile Enterotoxin in Colonization of the Adult Mouse Small Intestine by Human Enterotoxigenic Escherichia coli Strains |
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