Increased susceptibility of glutamine-depleted monocytes to fever-range hyperthermia: The role of 70-kDa heat shock protein

This study investigates the effect of fever-range hyperthermia on Gln-starving monocytes and the role of the 70-kDa heat shock protein Hsp70. Fever is a protective acute-phase response to infection. However, in critically ill patients, the harmful effects of fever seem to be predominant. Critical il...

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Veröffentlicht in:Annals of surgery 2005-02, Vol.241 (2), p.349-355
Hauptverfasser: POLLHEIMER, Jürgen, ZELLNER, Maria, ELIASEN, Maja Munk, ROTH, Erich, OEHLER, Rudolf
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container_end_page 355
container_issue 2
container_start_page 349
container_title Annals of surgery
container_volume 241
creator POLLHEIMER, Jürgen
ZELLNER, Maria
ELIASEN, Maja Munk
ROTH, Erich
OEHLER, Rudolf
description This study investigates the effect of fever-range hyperthermia on Gln-starving monocytes and the role of the 70-kDa heat shock protein Hsp70. Fever is a protective acute-phase response to infection. However, in critically ill patients, the harmful effects of fever seem to be predominant. Critical illness is frequently associated with reduced plasma glutamine (Gln) levels, which contribute to the immune suppression in these patients due to impaired monocyte function. Isolated monocytes were suspended in Gln-depleted medium and exposed to 41 degrees C. Cell survival was determined by an MTT-based assay, and phagocytosis of Escherichia coli was measured by flow cytometry. Expression of Hsp70 was determined by Western blot. Hyperthermia for 300 minutes strongly decreased the viability of Gln-depleted monocytes (85%), whereas it had only a moderate effect on Gln-supplied cells (45%, P < 0.05). Shorter treatments (45 minutes) of Gln-starving monocytes had almost no effects on viability but decreased the phagocytosis activity by 30.8%. In addition, the expression of Hsp70 was inhibited almost completely. These data show that Gln-starving monocytes have a reduced thermoresistance. This suggests that elevated body temperature damages monocytes in critically ill patients with reduced plasma Gln-levels possibly via an inhibition of the cytoprotective protein Hsp70.
doi_str_mv 10.1097/01.sla.0000152028.19115.27
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Fever is a protective acute-phase response to infection. However, in critically ill patients, the harmful effects of fever seem to be predominant. Critical illness is frequently associated with reduced plasma glutamine (Gln) levels, which contribute to the immune suppression in these patients due to impaired monocyte function. Isolated monocytes were suspended in Gln-depleted medium and exposed to 41 degrees C. Cell survival was determined by an MTT-based assay, and phagocytosis of Escherichia coli was measured by flow cytometry. Expression of Hsp70 was determined by Western blot. Hyperthermia for 300 minutes strongly decreased the viability of Gln-depleted monocytes (85%), whereas it had only a moderate effect on Gln-supplied cells (45%, P &lt; 0.05). Shorter treatments (45 minutes) of Gln-starving monocytes had almost no effects on viability but decreased the phagocytosis activity by 30.8%. In addition, the expression of Hsp70 was inhibited almost completely. 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subjects Biological and medical sciences
Cell Survival - physiology
Fever - physiopathology
Flow Cytometry
General aspects
Glutamine - blood
HSP70 Heat-Shock Proteins - physiology
Humans
Medical sciences
Monocytes - physiology
Original
Phagocytosis - physiology
title Increased susceptibility of glutamine-depleted monocytes to fever-range hyperthermia: The role of 70-kDa heat shock protein
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