Kininogenase of the guinea-pig's coagulating gland and the release of bradykinin
A kininogenase (CGK) in the coagulating gland of the guinea-pig was partially purified, characterized and separated from a kininase also present in the gland. This kininogenase is not inhibited by the usual kininogenase and protease inhibitors. Our results indicate that it is identical with the este...
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| Veröffentlicht in: | The Journal of physiology 1971-12, Vol.219 (2), p.341-353 |
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| container_title | The Journal of physiology |
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| creator | Moriwaki, C. Schachter, M. |
| description | A kininogenase (CGK) in the coagulating gland of the guinea-pig was partially purified, characterized and separated from a
kininase also present in the gland. This kininogenase is not inhibited by the usual kininogenase and protease inhibitors.
Our results indicate that it is identical with the esterolytic enzyme in the gland which hydrolyses the synthetic ester TAME.
The kinin released by CGK from dog's crude pseudoglobulin was purified more than 1500 times and identified as bradykinin by
chromatography on paper, on carboxymethyl-cellulose columns, and on silica gel. |
| doi_str_mv | 10.1113/jphysiol.1971.sp009665 |
| format | Article |
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kininase also present in the gland. This kininogenase is not inhibited by the usual kininogenase and protease inhibitors.
Our results indicate that it is identical with the esterolytic enzyme in the gland which hydrolyses the synthetic ester TAME.
The kinin released by CGK from dog's crude pseudoglobulin was purified more than 1500 times and identified as bradykinin by
chromatography on paper, on carboxymethyl-cellulose columns, and on silica gel.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.1971.sp009665</identifier><identifier>PMID: 5158391</identifier><language>eng</language><publisher>England: The Physiological Society</publisher><subject>Animals ; Arginine ; Bradykinin ; Chromatography ; Chromatography, Gel ; Chromatography, Paper ; Chromatography, Thin Layer ; Endopeptidases - isolation & purification ; Esters ; Genitalia, Male - enzymology ; Guinea Pigs ; Male ; Methylcellulose ; Protease Inhibitors ; Serum Globulins ; Tosyl Compounds</subject><ispartof>The Journal of physiology, 1971-12, Vol.219 (2), p.341-353</ispartof><rights>1971 The Physiological Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5001-6f358ff99061d427f461c2321da51992f4d0b062387d14c0042e30e7ee3fd48c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1331634/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1331634/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,1418,27928,27929,45578,45579,53795,53797</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5158391$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moriwaki, C.</creatorcontrib><creatorcontrib>Schachter, M.</creatorcontrib><title>Kininogenase of the guinea-pig's coagulating gland and the release of bradykinin</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>A kininogenase (CGK) in the coagulating gland of the guinea-pig was partially purified, characterized and separated from a
kininase also present in the gland. This kininogenase is not inhibited by the usual kininogenase and protease inhibitors.
Our results indicate that it is identical with the esterolytic enzyme in the gland which hydrolyses the synthetic ester TAME.
The kinin released by CGK from dog's crude pseudoglobulin was purified more than 1500 times and identified as bradykinin by
chromatography on paper, on carboxymethyl-cellulose columns, and on silica gel.</description><subject>Animals</subject><subject>Arginine</subject><subject>Bradykinin</subject><subject>Chromatography</subject><subject>Chromatography, Gel</subject><subject>Chromatography, Paper</subject><subject>Chromatography, Thin Layer</subject><subject>Endopeptidases - isolation & purification</subject><subject>Esters</subject><subject>Genitalia, Male - enzymology</subject><subject>Guinea Pigs</subject><subject>Male</subject><subject>Methylcellulose</subject><subject>Protease Inhibitors</subject><subject>Serum Globulins</subject><subject>Tosyl Compounds</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU-P1SAUxYnRjM_Rj6Dpyln1yQVKy8ZEJ_6fxFmMa8Kjl5axr1RonfTb26ZvJrpzQUg45_y4cAh5BXQPAPzN7dDOyYduD6qEfRooVVIWj8gOhFR5WSr-mOwoZSznZQFPybOUbikFTpU6I2cFFBVXsCPX33zv-9BgbxJmwWVji1kz-R5NPvjmImU2mGbqzOj7Jms609fZulZbxA5PqUM09fxzRT0nT5zpEr447efkx8cPN5ef86vvn75cvrvKbbGMkUvHi8o5paiEWrDSCQmWcQa1KUAp5kRND1QyXpU1CEupYMgplojc1aKy_Jy83bjDdDhibbEfo-n0EP3RxFkH4_W_Su9b3YTfGjgHycUCeH0CxPBrwjTqo08Wu-WJGKakK2BCSUEXo9yMNoaUIrqHS4DqtQt934Veu9D3XSzBl3-P-BA7ff6iv9_0O9_h_J9UffP1ej1goBgXK-Rig7S-ae98RL3FUrAex1kvPs306vwDJjyrvw</recordid><startdate>19711201</startdate><enddate>19711201</enddate><creator>Moriwaki, C.</creator><creator>Schachter, M.</creator><general>The Physiological Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19711201</creationdate><title>Kininogenase of the guinea-pig's coagulating gland and the release of bradykinin</title><author>Moriwaki, C. ; Schachter, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5001-6f358ff99061d427f461c2321da51992f4d0b062387d14c0042e30e7ee3fd48c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Animals</topic><topic>Arginine</topic><topic>Bradykinin</topic><topic>Chromatography</topic><topic>Chromatography, Gel</topic><topic>Chromatography, Paper</topic><topic>Chromatography, Thin Layer</topic><topic>Endopeptidases - isolation & purification</topic><topic>Esters</topic><topic>Genitalia, Male - enzymology</topic><topic>Guinea Pigs</topic><topic>Male</topic><topic>Methylcellulose</topic><topic>Protease Inhibitors</topic><topic>Serum Globulins</topic><topic>Tosyl Compounds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moriwaki, C.</creatorcontrib><creatorcontrib>Schachter, M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moriwaki, C.</au><au>Schachter, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Kininogenase of the guinea-pig's coagulating gland and the release of bradykinin</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1971-12-01</date><risdate>1971</risdate><volume>219</volume><issue>2</issue><spage>341</spage><epage>353</epage><pages>341-353</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><abstract>A kininogenase (CGK) in the coagulating gland of the guinea-pig was partially purified, characterized and separated from a
kininase also present in the gland. This kininogenase is not inhibited by the usual kininogenase and protease inhibitors.
Our results indicate that it is identical with the esterolytic enzyme in the gland which hydrolyses the synthetic ester TAME.
The kinin released by CGK from dog's crude pseudoglobulin was purified more than 1500 times and identified as bradykinin by
chromatography on paper, on carboxymethyl-cellulose columns, and on silica gel.</abstract><cop>England</cop><pub>The Physiological Society</pub><pmid>5158391</pmid><doi>10.1113/jphysiol.1971.sp009665</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3751 |
| ispartof | The Journal of physiology, 1971-12, Vol.219 (2), p.341-353 |
| issn | 0022-3751 1469-7793 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1331634 |
| source | MEDLINE; Access via Wiley Online Library; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Arginine Bradykinin Chromatography Chromatography, Gel Chromatography, Paper Chromatography, Thin Layer Endopeptidases - isolation & purification Esters Genitalia, Male - enzymology Guinea Pigs Male Methylcellulose Protease Inhibitors Serum Globulins Tosyl Compounds |
| title | Kininogenase of the guinea-pig's coagulating gland and the release of bradykinin |
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