The release of prostaglandins and progestin into the utero-ovarian venous blood of guinea-pigs during the oestrous cycle and following oestrogen treatment
1. Blood was collected from the utero-ovarian vein of guinea-pigs on days 3, 9, 10, 11, 12, 13, 14 and 15 of the oestrous cycle, and from animals injected with oestrogen over days 4-6 and used on day 7. The content of prostaglandin F 2α and E 2 was determined by bio-assay and gas chromatography-mas...
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| Veröffentlicht in: | The Journal of physiology 1972-05, Vol.223 (1), p.69-88 |
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| creator | Blatchley, F. R. Donovan, B. T. Horton, E. W. Poyser, N. L. |
| description | 1. Blood was collected from the utero-ovarian vein of guinea-pigs on days 3, 9, 10, 11, 12, 13, 14 and 15 of the oestrous
cycle, and from animals injected with oestrogen over days 4-6 and used on day 7. The content of prostaglandin F 2α and E 2 was determined by bio-assay and gas chromatography-mass spectrometry, and of progestin by competitive protein binding assay.
2. The concentration of prostaglandin F 2α on day 3 was 14·1 ng/ml.; it fell below detectable limits on day 7, but after day 11 the concentration rose to between 15·7-17·2
ng/ml. A value of 60·7 ng/ml. was recorded on day 15.
3. The release of prostaglandin F 2α was increased in animals pre-treated with oestrogen but was below threshold levels in similarly treated hysterectomized females.
4. Prostaglandin E 2 was not detectable in blood collected on days 3, 9, 10, 11 and 12. From a threshold value of 1·1 ng/ml. on day 13, the concentration
rose to 5·4 ng/ml. on day 14 and to 54·9 ng/ml. on day 15.
5. The progestin concentration rose from 66 ng/ml. on day 3 to values of 80-180 ng/ml. between days 9-14. It fell to 45 ng/ml.
between days 14 and 15.
6. This work establishes that prostaglandin F 2α is released into the utero-ovarian venous blood, and that its concentration fluctuates in the course of the oestrous cycle
and can be related to changes in luteal function. |
| doi_str_mv | 10.1113/jphysiol.1972.sp009835 |
| format | Article |
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cycle, and from animals injected with oestrogen over days 4-6 and used on day 7. The content of prostaglandin F 2α and E 2 was determined by bio-assay and gas chromatography-mass spectrometry, and of progestin by competitive protein binding assay.
2. The concentration of prostaglandin F 2α on day 3 was 14·1 ng/ml.; it fell below detectable limits on day 7, but after day 11 the concentration rose to between 15·7-17·2
ng/ml. A value of 60·7 ng/ml. was recorded on day 15.
3. The release of prostaglandin F 2α was increased in animals pre-treated with oestrogen but was below threshold levels in similarly treated hysterectomized females.
4. Prostaglandin E 2 was not detectable in blood collected on days 3, 9, 10, 11 and 12. From a threshold value of 1·1 ng/ml. on day 13, the concentration
rose to 5·4 ng/ml. on day 14 and to 54·9 ng/ml. on day 15.
5. The progestin concentration rose from 66 ng/ml. on day 3 to values of 80-180 ng/ml. between days 9-14. It fell to 45 ng/ml.
between days 14 and 15.
6. This work establishes that prostaglandin F 2α is released into the utero-ovarian venous blood, and that its concentration fluctuates in the course of the oestrous cycle
and can be related to changes in luteal function.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.1972.sp009835</identifier><identifier>PMID: 5065267</identifier><language>eng</language><publisher>England: The Physiological Society</publisher><subject>Animals ; Biological Assay ; Chromatography, Gas ; Corpus Luteum - physiology ; Estrogens - pharmacology ; Estrus ; Female ; Guinea Pigs ; Hysterectomy ; Ovary - blood supply ; Pregnancy ; Progesterone - blood ; Progesterone - physiology ; Prostaglandins - analysis ; Prostaglandins - blood ; Prostaglandins - physiology ; Spectrum Analysis ; Time Factors ; Uterus - blood supply ; Veins</subject><ispartof>The Journal of physiology, 1972-05, Vol.223 (1), p.69-88</ispartof><rights>1972 The Physiological Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4139-83aa95046fc36aed8003551323c311ace89700ccc7b16f23f2ae9742e3d01c383</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1331434/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1331434/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,729,782,786,887,1419,27933,27934,45583,45584,53800,53802</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5065267$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Blatchley, F. R.</creatorcontrib><creatorcontrib>Donovan, B. T.</creatorcontrib><creatorcontrib>Horton, E. W.</creatorcontrib><creatorcontrib>Poyser, N. L.</creatorcontrib><title>The release of prostaglandins and progestin into the utero-ovarian venous blood of guinea-pigs during the oestrous cycle and following oestrogen treatment</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>1. Blood was collected from the utero-ovarian vein of guinea-pigs on days 3, 9, 10, 11, 12, 13, 14 and 15 of the oestrous
cycle, and from animals injected with oestrogen over days 4-6 and used on day 7. The content of prostaglandin F 2α and E 2 was determined by bio-assay and gas chromatography-mass spectrometry, and of progestin by competitive protein binding assay.
2. The concentration of prostaglandin F 2α on day 3 was 14·1 ng/ml.; it fell below detectable limits on day 7, but after day 11 the concentration rose to between 15·7-17·2
ng/ml. A value of 60·7 ng/ml. was recorded on day 15.
3. The release of prostaglandin F 2α was increased in animals pre-treated with oestrogen but was below threshold levels in similarly treated hysterectomized females.
4. Prostaglandin E 2 was not detectable in blood collected on days 3, 9, 10, 11 and 12. From a threshold value of 1·1 ng/ml. on day 13, the concentration
rose to 5·4 ng/ml. on day 14 and to 54·9 ng/ml. on day 15.
5. The progestin concentration rose from 66 ng/ml. on day 3 to values of 80-180 ng/ml. between days 9-14. It fell to 45 ng/ml.
between days 14 and 15.
6. This work establishes that prostaglandin F 2α is released into the utero-ovarian venous blood, and that its concentration fluctuates in the course of the oestrous cycle
and can be related to changes in luteal function.</description><subject>Animals</subject><subject>Biological Assay</subject><subject>Chromatography, Gas</subject><subject>Corpus Luteum - physiology</subject><subject>Estrogens - pharmacology</subject><subject>Estrus</subject><subject>Female</subject><subject>Guinea Pigs</subject><subject>Hysterectomy</subject><subject>Ovary - blood supply</subject><subject>Pregnancy</subject><subject>Progesterone - blood</subject><subject>Progesterone - physiology</subject><subject>Prostaglandins - analysis</subject><subject>Prostaglandins - blood</subject><subject>Prostaglandins - physiology</subject><subject>Spectrum Analysis</subject><subject>Time Factors</subject><subject>Uterus - blood supply</subject><subject>Veins</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1972</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUctu1DAUtRCoTAufAPKKXQY_8vIGqap4FFWCxbC2PM5N4spjBzuZUX6Fr8VppgV2rK50z-M-DkJvKdlSSvn7-6Gfo_F2S0XFtnEgRNS8eIY2NC9FVlWCP0cbQhjLeFXQl-gyxntCKCdCXKCLgpQFK6sN-rXrAQewoCJg3-Ih-DiqzirXGBdxKkurgzgah40bPR6TYBoh-MwfVTDK4SM4P0W8t943i0c3GQcqG0wXcTMF47oHkU8mYSHqWVt4sG69tf60EFawA4fHAGo8gBtfoRetshFen-sV-vHp4-7mS3b37fPtzfVdpnPKRVZzpURB8rLVvFTQ1ITwoqCccc0pVRpqURGita72tGwZb5kCUeUMeEOo5jW_Qh9W32HaH6DRaXRQVg7BHFSYpVdG_os408vOHyXlnOY8TwbvzgbB_5zSIfJgogabngjpXlnTIs9ZVSRiuRJ1-nIM0D4NoUQuqcrHVOWSqnxMNQnf_L3ik-wcY8KvV_xkLMz_6Sp3X78vDcY4LcWfK3rT9ScTQK6q6LWBcZaJJqlMxN-zI8jg</recordid><startdate>19720501</startdate><enddate>19720501</enddate><creator>Blatchley, F. R.</creator><creator>Donovan, B. T.</creator><creator>Horton, E. W.</creator><creator>Poyser, N. L.</creator><general>The Physiological Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19720501</creationdate><title>The release of prostaglandins and progestin into the utero-ovarian venous blood of guinea-pigs during the oestrous cycle and following oestrogen treatment</title><author>Blatchley, F. R. ; Donovan, B. T. ; Horton, E. W. ; Poyser, N. L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4139-83aa95046fc36aed8003551323c311ace89700ccc7b16f23f2ae9742e3d01c383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1972</creationdate><topic>Animals</topic><topic>Biological Assay</topic><topic>Chromatography, Gas</topic><topic>Corpus Luteum - physiology</topic><topic>Estrogens - pharmacology</topic><topic>Estrus</topic><topic>Female</topic><topic>Guinea Pigs</topic><topic>Hysterectomy</topic><topic>Ovary - blood supply</topic><topic>Pregnancy</topic><topic>Progesterone - blood</topic><topic>Progesterone - physiology</topic><topic>Prostaglandins - analysis</topic><topic>Prostaglandins - blood</topic><topic>Prostaglandins - physiology</topic><topic>Spectrum Analysis</topic><topic>Time Factors</topic><topic>Uterus - blood supply</topic><topic>Veins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Blatchley, F. R.</creatorcontrib><creatorcontrib>Donovan, B. T.</creatorcontrib><creatorcontrib>Horton, E. W.</creatorcontrib><creatorcontrib>Poyser, N. L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blatchley, F. R.</au><au>Donovan, B. T.</au><au>Horton, E. W.</au><au>Poyser, N. L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The release of prostaglandins and progestin into the utero-ovarian venous blood of guinea-pigs during the oestrous cycle and following oestrogen treatment</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1972-05-01</date><risdate>1972</risdate><volume>223</volume><issue>1</issue><spage>69</spage><epage>88</epage><pages>69-88</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><abstract>1. Blood was collected from the utero-ovarian vein of guinea-pigs on days 3, 9, 10, 11, 12, 13, 14 and 15 of the oestrous
cycle, and from animals injected with oestrogen over days 4-6 and used on day 7. The content of prostaglandin F 2α and E 2 was determined by bio-assay and gas chromatography-mass spectrometry, and of progestin by competitive protein binding assay.
2. The concentration of prostaglandin F 2α on day 3 was 14·1 ng/ml.; it fell below detectable limits on day 7, but after day 11 the concentration rose to between 15·7-17·2
ng/ml. A value of 60·7 ng/ml. was recorded on day 15.
3. The release of prostaglandin F 2α was increased in animals pre-treated with oestrogen but was below threshold levels in similarly treated hysterectomized females.
4. Prostaglandin E 2 was not detectable in blood collected on days 3, 9, 10, 11 and 12. From a threshold value of 1·1 ng/ml. on day 13, the concentration
rose to 5·4 ng/ml. on day 14 and to 54·9 ng/ml. on day 15.
5. The progestin concentration rose from 66 ng/ml. on day 3 to values of 80-180 ng/ml. between days 9-14. It fell to 45 ng/ml.
between days 14 and 15.
6. This work establishes that prostaglandin F 2α is released into the utero-ovarian venous blood, and that its concentration fluctuates in the course of the oestrous cycle
and can be related to changes in luteal function.</abstract><cop>England</cop><pub>The Physiological Society</pub><pmid>5065267</pmid><doi>10.1113/jphysiol.1972.sp009835</doi><tpages>20</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via Wiley Online Library; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Biological Assay Chromatography, Gas Corpus Luteum - physiology Estrogens - pharmacology Estrus Female Guinea Pigs Hysterectomy Ovary - blood supply Pregnancy Progesterone - blood Progesterone - physiology Prostaglandins - analysis Prostaglandins - blood Prostaglandins - physiology Spectrum Analysis Time Factors Uterus - blood supply Veins |
| title | The release of prostaglandins and progestin into the utero-ovarian venous blood of guinea-pigs during the oestrous cycle and following oestrogen treatment |
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