Repair of radiation-induced DNA damage in nondividing populations of human diploid fibroblasts
The occurrence of DNA repair in UV- (254 nm) and X-irradiated normal human diploid fibroblasts maintained in a quiescent, nondividing state using low serum (0.5%) medium was ascertained. Techniques that detect different steps of the excision repair process were used so that the extent of completion...
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| Veröffentlicht in: | Biophys. J.; (United States) 1980-06, Vol.30 (3), p.399-413 |
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| creator | Kantor, G.J. Petty, R.S. Warner, C. Phillips, D.J. Hull, D.R. |
| description | The occurrence of DNA repair in UV- (254 nm) and X-irradiated normal human diploid fibroblasts maintained in a quiescent, nondividing state using low serum (0.5%) medium was ascertained. Techniques that detect different steps of the excision repair process were used so that the extent of completion of repair at single sites could be determined. These included measuring the disappearance of pyrimidine dimers by chromatography, detecting repair synthesis by density-gradient and autoradiographic methods and detecting the rejoining of repaired regions and repair of x-ray-induced single-strand DNA breaks using alkaline sucrose gradients. Results show that dimer excision occurs and the subsequent steps of repair synthesis and ligation are completed. About 50% of the dimers formed by exposure to 20 J/m2 is excised in the initial 24-h post-UV period. DNA repair (unscheduled DNA synthesis) can be detected through a 5-d post-UV period. The fraction of damaged sites eventually repaired is not known. X-ray-induced single-strand DNA breaks are repaired rapidly. |
| doi_str_mv | 10.1016/S0006-3495(80)85104-6 |
| format | Article |
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Techniques that detect different steps of the excision repair process were used so that the extent of completion of repair at single sites could be determined. These included measuring the disappearance of pyrimidine dimers by chromatography, detecting repair synthesis by density-gradient and autoradiographic methods and detecting the rejoining of repaired regions and repair of x-ray-induced single-strand DNA breaks using alkaline sucrose gradients. Results show that dimer excision occurs and the subsequent steps of repair synthesis and ligation are completed. About 50% of the dimers formed by exposure to 20 J/m2 is excised in the initial 24-h post-UV period. DNA repair (unscheduled DNA synthesis) can be detected through a 5-d post-UV period. The fraction of damaged sites eventually repaired is not known. X-ray-induced single-strand DNA breaks are repaired rapidly.</description><identifier>ISSN: 0006-3495</identifier><identifier>EISSN: 1542-0086</identifier><identifier>DOI: 10.1016/S0006-3495(80)85104-6</identifier><identifier>PMID: 7260281</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>560110 - Radiation Effects on Biochemicals- (-1987) ; 560121 - Radiation Effects on Cells- External Source- (-1987) ; ANIMAL CELLS ; ANIMALS ; AZINES ; BIOLOGICAL EFFECTS ; BIOLOGICAL RADIATION EFFECTS ; BIOLOGICAL RECOVERY ; BIOLOGICAL REPAIR ; CELL CULTURES ; Cell Line ; CONNECTIVE TISSUE CELLS ; DIMERS ; DIPLOIDY ; DNA ; DNA - radiation effects ; DNA Repair ; ELECTROMAGNETIC RADIATION ; FIBROBLASTS ; Fibroblasts - radiation effects ; HETEROCYCLIC COMPOUNDS ; Humans ; IONIZING RADIATIONS ; Kinetics ; MAMMALS ; MAN ; NUCLEIC ACIDS ; ORGANIC COMPOUNDS ; ORGANIC NITROGEN COMPOUNDS ; PLOIDY ; PRIMATES ; Pyrimidine Dimers - radiation effects ; PYRIMIDINES ; RADIATION EFFECTS ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RADIATIONS ; RECOVERY ; REPAIR ; SOMATIC CELLS ; STRAND BREAKS ; TIME DEPENDENCE ; ULTRAVIOLET RADIATION ; Ultraviolet Rays ; VERTEBRATES ; X RADIATION</subject><ispartof>Biophys. 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J.; (United States)</title><addtitle>Biophys J</addtitle><description>The occurrence of DNA repair in UV- (254 nm) and X-irradiated normal human diploid fibroblasts maintained in a quiescent, nondividing state using low serum (0.5%) medium was ascertained. Techniques that detect different steps of the excision repair process were used so that the extent of completion of repair at single sites could be determined. These included measuring the disappearance of pyrimidine dimers by chromatography, detecting repair synthesis by density-gradient and autoradiographic methods and detecting the rejoining of repaired regions and repair of x-ray-induced single-strand DNA breaks using alkaline sucrose gradients. Results show that dimer excision occurs and the subsequent steps of repair synthesis and ligation are completed. About 50% of the dimers formed by exposure to 20 J/m2 is excised in the initial 24-h post-UV period. DNA repair (unscheduled DNA synthesis) can be detected through a 5-d post-UV period. The fraction of damaged sites eventually repaired is not known. X-ray-induced single-strand DNA breaks are repaired rapidly.</description><subject>560110 - Radiation Effects on Biochemicals- (-1987)</subject><subject>560121 - Radiation Effects on Cells- External Source- (-1987)</subject><subject>ANIMAL CELLS</subject><subject>ANIMALS</subject><subject>AZINES</subject><subject>BIOLOGICAL EFFECTS</subject><subject>BIOLOGICAL RADIATION EFFECTS</subject><subject>BIOLOGICAL RECOVERY</subject><subject>BIOLOGICAL REPAIR</subject><subject>CELL CULTURES</subject><subject>Cell Line</subject><subject>CONNECTIVE TISSUE CELLS</subject><subject>DIMERS</subject><subject>DIPLOIDY</subject><subject>DNA</subject><subject>DNA - radiation effects</subject><subject>DNA Repair</subject><subject>ELECTROMAGNETIC RADIATION</subject><subject>FIBROBLASTS</subject><subject>Fibroblasts - radiation effects</subject><subject>HETEROCYCLIC COMPOUNDS</subject><subject>Humans</subject><subject>IONIZING RADIATIONS</subject><subject>Kinetics</subject><subject>MAMMALS</subject><subject>MAN</subject><subject>NUCLEIC ACIDS</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANIC NITROGEN COMPOUNDS</subject><subject>PLOIDY</subject><subject>PRIMATES</subject><subject>Pyrimidine Dimers - radiation effects</subject><subject>PYRIMIDINES</subject><subject>RADIATION EFFECTS</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RADIATIONS</subject><subject>RECOVERY</subject><subject>REPAIR</subject><subject>SOMATIC CELLS</subject><subject>STRAND BREAKS</subject><subject>TIME DEPENDENCE</subject><subject>ULTRAVIOLET RADIATION</subject><subject>Ultraviolet Rays</subject><subject>VERTEBRATES</subject><subject>X RADIATION</subject><issn>0006-3495</issn><issn>1542-0086</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUmP1DAQhS0EGpqBnzBSxAHBIVCOlyQX0GhYpRFILFcsx0t3ocTO2ElL_HuS7lYLTpx8eK_eq_JHyBWFlxSofPUNAGTJeCueN_CiERR4Ke-RDRW8KgEaeZ9szpaH5FHOvwBoJYBekIu6klA1dEN-fnWjxlREXyRtUU8YQ4nBzsbZ4u3n68LqQW9dgaEIMVjco8WwLcY4zv3BnNfR3TzoUFgc-4i28Nil2PU6T_kxeeB1n92T03tJfrx_9_3mY3n75cOnm-vb0vCmnUpJGatqznTVWe9ayiwTnreVq-uWdlw7X4O2ousqBrYWUmjOvZeNYR58Jyi7JK-PuePcDc4aF6akezUmHHT6raJG9a8ScKe2ca8oq5qa10vA02NAzBOqbHByZmdiCM5MSkDbSCYX07NTS4p3s8uTGjAb1_c6uDhnVQvBuRTtYhRHo0kx5-T8eRMKaqWnDvTUikY1oA701Fpw9fcZ56kTrkV_c9Td8pd7dGnd1IWFFaZ1URvxPw1_ALqrqzE</recordid><startdate>19800601</startdate><enddate>19800601</enddate><creator>Kantor, G.J.</creator><creator>Petty, R.S.</creator><creator>Warner, C.</creator><creator>Phillips, D.J.</creator><creator>Hull, D.R.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>19800601</creationdate><title>Repair of radiation-induced DNA damage in nondividing populations of human diploid fibroblasts</title><author>Kantor, G.J. ; Petty, R.S. ; Warner, C. ; Phillips, D.J. ; Hull, D.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c489t-61332743a2bdfe913d35f492e7791b4aef70ad5bb230d7565a44ff68c3f0fb513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>560110 - Radiation Effects on Biochemicals- (-1987)</topic><topic>560121 - Radiation Effects on Cells- External Source- (-1987)</topic><topic>ANIMAL CELLS</topic><topic>ANIMALS</topic><topic>AZINES</topic><topic>BIOLOGICAL EFFECTS</topic><topic>BIOLOGICAL RADIATION EFFECTS</topic><topic>BIOLOGICAL RECOVERY</topic><topic>BIOLOGICAL REPAIR</topic><topic>CELL CULTURES</topic><topic>Cell Line</topic><topic>CONNECTIVE TISSUE CELLS</topic><topic>DIMERS</topic><topic>DIPLOIDY</topic><topic>DNA</topic><topic>DNA - radiation effects</topic><topic>DNA Repair</topic><topic>ELECTROMAGNETIC RADIATION</topic><topic>FIBROBLASTS</topic><topic>Fibroblasts - radiation effects</topic><topic>HETEROCYCLIC COMPOUNDS</topic><topic>Humans</topic><topic>IONIZING RADIATIONS</topic><topic>Kinetics</topic><topic>MAMMALS</topic><topic>MAN</topic><topic>NUCLEIC ACIDS</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANIC NITROGEN COMPOUNDS</topic><topic>PLOIDY</topic><topic>PRIMATES</topic><topic>Pyrimidine Dimers - radiation effects</topic><topic>PYRIMIDINES</topic><topic>RADIATION EFFECTS</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. 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J.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kantor, G.J.</au><au>Petty, R.S.</au><au>Warner, C.</au><au>Phillips, D.J.</au><au>Hull, D.R.</au><aucorp>Wright State Univ., Dayton, OH</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Repair of radiation-induced DNA damage in nondividing populations of human diploid fibroblasts</atitle><jtitle>Biophys. J.; (United States)</jtitle><addtitle>Biophys J</addtitle><date>1980-06-01</date><risdate>1980</risdate><volume>30</volume><issue>3</issue><spage>399</spage><epage>413</epage><pages>399-413</pages><issn>0006-3495</issn><eissn>1542-0086</eissn><abstract>The occurrence of DNA repair in UV- (254 nm) and X-irradiated normal human diploid fibroblasts maintained in a quiescent, nondividing state using low serum (0.5%) medium was ascertained. Techniques that detect different steps of the excision repair process were used so that the extent of completion of repair at single sites could be determined. These included measuring the disappearance of pyrimidine dimers by chromatography, detecting repair synthesis by density-gradient and autoradiographic methods and detecting the rejoining of repaired regions and repair of x-ray-induced single-strand DNA breaks using alkaline sucrose gradients. Results show that dimer excision occurs and the subsequent steps of repair synthesis and ligation are completed. About 50% of the dimers formed by exposure to 20 J/m2 is excised in the initial 24-h post-UV period. DNA repair (unscheduled DNA synthesis) can be detected through a 5-d post-UV period. The fraction of damaged sites eventually repaired is not known. X-ray-induced single-strand DNA breaks are repaired rapidly.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7260281</pmid><doi>10.1016/S0006-3495(80)85104-6</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biophys. J.; (United States), 1980-06, Vol.30 (3), p.399-413 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Open Access: Cell Press Free Archives; PubMed Central; EZB Electronic Journals Library |
| subjects | 560110 - Radiation Effects on Biochemicals- (-1987) 560121 - Radiation Effects on Cells- External Source- (-1987) ANIMAL CELLS ANIMALS AZINES BIOLOGICAL EFFECTS BIOLOGICAL RADIATION EFFECTS BIOLOGICAL RECOVERY BIOLOGICAL REPAIR CELL CULTURES Cell Line CONNECTIVE TISSUE CELLS DIMERS DIPLOIDY DNA DNA - radiation effects DNA Repair ELECTROMAGNETIC RADIATION FIBROBLASTS Fibroblasts - radiation effects HETEROCYCLIC COMPOUNDS Humans IONIZING RADIATIONS Kinetics MAMMALS MAN NUCLEIC ACIDS ORGANIC COMPOUNDS ORGANIC NITROGEN COMPOUNDS PLOIDY PRIMATES Pyrimidine Dimers - radiation effects PYRIMIDINES RADIATION EFFECTS RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIATIONS RECOVERY REPAIR SOMATIC CELLS STRAND BREAKS TIME DEPENDENCE ULTRAVIOLET RADIATION Ultraviolet Rays VERTEBRATES X RADIATION |
| title | Repair of radiation-induced DNA damage in nondividing populations of human diploid fibroblasts |
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