Antigenic analyses of tissues and excretory and secretory products from Strongylus vulgaris
Rabbit antisera were prepared against veronal buffered saline extracts of L4 and L5 Strongylus vulgaris, adult S. vulgaris and adult Strongylus equinus retrieved from naturally infected horses. In agar gel diffusion with these antisera, adult S vulgaris and S. equinus each appeared to have at least...
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Veröffentlicht in: | Canadian journal of comparative medicine 1981-07, Vol.45 (3), p.259-265 |
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creator | Wynne, E Slocome, J.O.D Wilkie, B.N |
description | Rabbit antisera were prepared against veronal buffered saline extracts of L4 and L5 Strongylus vulgaris, adult S. vulgaris and adult Strongylus equinus retrieved from naturally infected horses. In agar gel diffusion with these antisera, adult S vulgaris and S. equinus each appeared to have at least one unique antigen; larval S. vulgaris appeared to have two species-specific and two stage-specific antigens. There were several common antigens. Excretory and secretory products were collected also from L4 and L5 an maintained over several days in tissue culture fluid. In agar gel diffusion against the above rabbit antisera, a stage-specific antigen was found also in excretory and secretory products. In addition, excretory and secretory products had three antigens in common with adult and larval S. vulgaris, but only one of these was common to adult S. equinus. The excretory and secretory products appear, therefore, to have two species-specific and one stage-specific antigens. |
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In agar gel diffusion with these antisera, adult S vulgaris and S. equinus each appeared to have at least one unique antigen; larval S. vulgaris appeared to have two species-specific and two stage-specific antigens. There were several common antigens. Excretory and secretory products were collected also from L4 and L5 an maintained over several days in tissue culture fluid. In agar gel diffusion against the above rabbit antisera, a stage-specific antigen was found also in excretory and secretory products. In addition, excretory and secretory products had three antigens in common with adult and larval S. vulgaris, but only one of these was common to adult S. equinus. 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In agar gel diffusion with these antisera, adult S vulgaris and S. equinus each appeared to have at least one unique antigen; larval S. vulgaris appeared to have two species-specific and two stage-specific antigens. There were several common antigens. Excretory and secretory products were collected also from L4 and L5 an maintained over several days in tissue culture fluid. In agar gel diffusion against the above rabbit antisera, a stage-specific antigen was found also in excretory and secretory products. In addition, excretory and secretory products had three antigens in common with adult and larval S. vulgaris, but only one of these was common to adult S. equinus. The excretory and secretory products appear, therefore, to have two species-specific and one stage-specific antigens.</description><subject>Animals</subject><subject>Antigens - analysis</subject><subject>Antigens - immunology</subject><subject>Antigens - isolation & purification</subject><subject>Cecum - immunology</subject><subject>Hemagglutination Tests</subject><subject>horses</subject><subject>Horses - immunology</subject><subject>Immunodiffusion</subject><subject>Strongyle Infections, Equine - immunology</subject><subject>Strongyloidea - immunology</subject><issn>0008-4050</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUMtOwzAQzAFUSuETEDlxi7R2bCe5IFUVL6kSh9ITB8uN7WCU2MV2Kvr3WLQgOO2OZjSzOyfZFADqggCFs-w8hHcAUrKGTbIJq4FABdPsdW6j6ZQ1bS6s6PdBhdzpPJoQxrQKK3P12XoVnd9_o6B-0NY7ObYx5Nq7IV9F72y378eQ78a-E96Ei-xUiz6oy-OcZev7u5fFY7F8fnhazJeFxjWNBVN1pRljVEqBKMKKICwIE1I2Kim0YiTd2m4orTDaEIHqCivJWClAgSasnGW3B9_tuBmUbJWNXvR8680g_J47Yfh_xpo33rkdRyUGjKpkcHM08O4jvR35YEKr-l5Y5cbAq7KpCG7qJLz6m_Qbcawz8dcHXgvHRZc64OsVBlQCroE2DS2_ANakfIw</recordid><startdate>19810701</startdate><enddate>19810701</enddate><creator>Wynne, E</creator><creator>Slocome, J.O.D</creator><creator>Wilkie, B.N</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19810701</creationdate><title>Antigenic analyses of tissues and excretory and secretory products from Strongylus vulgaris</title><author>Wynne, E ; Slocome, J.O.D ; Wilkie, B.N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f285t-6e87f6665dda1512e412a46add9ef28fe64407cb55721b4a1872ed663a0e0f463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Animals</topic><topic>Antigens - analysis</topic><topic>Antigens - immunology</topic><topic>Antigens - isolation & purification</topic><topic>Cecum - immunology</topic><topic>Hemagglutination Tests</topic><topic>horses</topic><topic>Horses - immunology</topic><topic>Immunodiffusion</topic><topic>Strongyle Infections, Equine - immunology</topic><topic>Strongyloidea - immunology</topic><toplevel>online_resources</toplevel><creatorcontrib>Wynne, E</creatorcontrib><creatorcontrib>Slocome, J.O.D</creatorcontrib><creatorcontrib>Wilkie, B.N</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Canadian journal of comparative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wynne, E</au><au>Slocome, J.O.D</au><au>Wilkie, B.N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antigenic analyses of tissues and excretory and secretory products from Strongylus vulgaris</atitle><jtitle>Canadian journal of comparative medicine</jtitle><addtitle>Can J Comp Med</addtitle><date>1981-07-01</date><risdate>1981</risdate><volume>45</volume><issue>3</issue><spage>259</spage><epage>265</epage><pages>259-265</pages><issn>0008-4050</issn><abstract>Rabbit antisera were prepared against veronal buffered saline extracts of L4 and L5 Strongylus vulgaris, adult S. vulgaris and adult Strongylus equinus retrieved from naturally infected horses. In agar gel diffusion with these antisera, adult S vulgaris and S. equinus each appeared to have at least one unique antigen; larval S. vulgaris appeared to have two species-specific and two stage-specific antigens. There were several common antigens. Excretory and secretory products were collected also from L4 and L5 an maintained over several days in tissue culture fluid. In agar gel diffusion against the above rabbit antisera, a stage-specific antigen was found also in excretory and secretory products. In addition, excretory and secretory products had three antigens in common with adult and larval S. vulgaris, but only one of these was common to adult S. equinus. The excretory and secretory products appear, therefore, to have two species-specific and one stage-specific antigens.</abstract><cop>Canada</cop><pmid>6804070</pmid><tpages>7</tpages></addata></record> |
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subjects | Animals Antigens - analysis Antigens - immunology Antigens - isolation & purification Cecum - immunology Hemagglutination Tests horses Horses - immunology Immunodiffusion Strongyle Infections, Equine - immunology Strongyloidea - immunology |
title | Antigenic analyses of tissues and excretory and secretory products from Strongylus vulgaris |
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