pH Dependence Thermal Stability of a Chymotrypsin Inhibitor from Schizolobium parahyba Seeds

The thermal stability of a Schizolobium parahyba chymotrypsin inhibitor (SPCI) as a function of pH has been investigated using fluorescence, circular dichroism, and differential scanning calorimetry (DSC). The thermodynamic parameters derived from all methods are remarkably similar and strongly sugg...

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Veröffentlicht in:	Biophysical journal 2005-05, Vol.88 (5), p.3509-3517
Hauptverfasser:	Teles, Rozeni C.L., Calderon, Leonardo de A., Medrano, Francisco J., Barbosa, João A.R.G., Guimarães, Beatriz G., Santoro, Marcelo M., de Freitas, Sonia M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Calorimetry, Differential Scanning Chymotrypsin - antagonists & inhibitors Circular Dichroism Differential scanning calorimetry Fluorescence Histidine - chemistry Hot Temperature Hydrogen-Ion Concentration Plant Proteins - chemistry Plants - metabolism Protease inhibitors Protein Denaturation Protein Folding Proteins Seeds Spectrometry, Fluorescence Temperature Thermodynamics Ultraviolet Rays
Online-Zugang:	Volltext
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