Simultaneous Topography and Recognition Imaging Using Force Microscopy

We present a method for simultaneously recording topography images and localizing specific binding sites with nm positional accuracy by combining dynamic force microscopy with single molecule recognition force spectroscopy. For this we used lysozyme adsorbed to mica, the functionality of which was c...

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Veröffentlicht in:	Biophysical journal 2004-09, Vol.87 (3), p.1981-1990
Hauptverfasser:	Stroh, Cordula M., Ebner, Andreas, Geretschläger, Manfred, Freudenthaler, Günter, Kienberger, Ferry, Kamruzzahan, A.S.M., Smith-Gill, Sandra J., Gruber, Hermann J., Hinterdorfer, Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	Aluminum Silicates - chemistry Animals Antibodies - chemistry Antigen-Antibody Reactions Biochemistry Calibration Cartography Enzymes Image Processing, Computer-Assisted Immunoenzyme Techniques Microscopy Microscopy, Atomic Force - instrumentation Microscopy, Atomic Force - methods Muramidase - chemistry Muramidase - ultrastructure Oscillometry Software Spectroscopy, Imaging, Other Techniques Spectrum analysis Surface Properties Time Factors
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