Elastic Light Scattering from Single Cells: Orientational Dynamics in Optical Trap
Light-scattering diagrams (phase functions) from single living cells and beads suspended in an optical trap were recorded with 30-ms time resolution. The intensity of the scattered light was recorded over an angular range of 0.5–179.5° using an optical setup based on an elliptical mirror and rotatin...
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| Veröffentlicht in: | Biophysical journal 2004-08, Vol.87 (2), p.1298-1306 |
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| creator | Watson, Dakota Hagen, Norbert Diver, Jonathan Marchand, Philippe Chachisvilis, Mirianas |
| description | Light-scattering diagrams (phase functions) from single living cells and beads suspended in an optical trap were recorded with 30-ms time resolution. The intensity of the scattered light was recorded over an angular range of 0.5–179.5° using an optical setup based on an elliptical mirror and rotating aperture. Experiments revealed that light-scattering diagrams from biological cells exhibit significant and complex time dependence. We have attributed this dependence to the cell's orientational dynamics within the trap. We have also used experimentally measured phase function information to calculate the time dependence of the optical radiation pressure force on the trapped particle and show how it changes depending on the orientation of the particle. Relevance of these experiments to potential improvement in the sensitivity of label-free flow cytometry is discussed. |
| doi_str_mv | 10.1529/biophysj.104.042135 |
| format | Article |
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The intensity of the scattered light was recorded over an angular range of 0.5–179.5° using an optical setup based on an elliptical mirror and rotating aperture. Experiments revealed that light-scattering diagrams from biological cells exhibit significant and complex time dependence. We have attributed this dependence to the cell's orientational dynamics within the trap. We have also used experimentally measured phase function information to calculate the time dependence of the optical radiation pressure force on the trapped particle and show how it changes depending on the orientation of the particle. Relevance of these experiments to potential improvement in the sensitivity of label-free flow cytometry is discussed.</description><identifier>ISSN: 0006-3495</identifier><identifier>EISSN: 1542-0086</identifier><identifier>DOI: 10.1529/biophysj.104.042135</identifier><identifier>PMID: 15298932</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Biochemistry ; Cell Polarity - physiology ; Cells, Cultured ; Cellular biology ; Diffusion ; Elasticity ; Equipment Design ; Equipment Failure Analysis ; Flow Cytometry - instrumentation ; Flow Cytometry - methods ; Fluorescence ; Granulocytes - cytology ; Humans ; Leukocytes, Mononuclear - cytology ; Melanoma - pathology ; Micromanipulation - instrumentation ; Micromanipulation - methods ; Microscopy, Polarization - instrumentation ; Microscopy, Polarization - methods ; Optics and Photonics - instrumentation ; Phantoms, Imaging ; Physical Stimulation - instrumentation ; Physical Stimulation - methods ; Refractometry - instrumentation ; Refractometry - methods ; Reproducibility of Results ; Scatter diagrams ; Scattering, Radiation ; Sensitivity and Specificity ; Spectroscopy, Imaging, Other Techniques ; Spectrum analysis</subject><ispartof>Biophysical journal, 2004-08, Vol.87 (2), p.1298-1306</ispartof><rights>2004 The Biophysical Society</rights><rights>Copyright Biophysical Society Aug 2004</rights><rights>Copyright © 2004, Biophysical Society 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-17b4135291552ee10dcc273ce52dbc12941bae20ec035b612ebda2c8bee2fc033</citedby><cites>FETCH-LOGICAL-c482t-17b4135291552ee10dcc273ce52dbc12941bae20ec035b612ebda2c8bee2fc033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1304468/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1529/biophysj.104.042135$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,3550,27924,27925,45995,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15298932$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Watson, Dakota</creatorcontrib><creatorcontrib>Hagen, Norbert</creatorcontrib><creatorcontrib>Diver, Jonathan</creatorcontrib><creatorcontrib>Marchand, Philippe</creatorcontrib><creatorcontrib>Chachisvilis, Mirianas</creatorcontrib><title>Elastic Light Scattering from Single Cells: Orientational Dynamics in Optical Trap</title><title>Biophysical journal</title><addtitle>Biophys J</addtitle><description>Light-scattering diagrams (phase functions) from single living cells and beads suspended in an optical trap were recorded with 30-ms time resolution. The intensity of the scattered light was recorded over an angular range of 0.5–179.5° using an optical setup based on an elliptical mirror and rotating aperture. Experiments revealed that light-scattering diagrams from biological cells exhibit significant and complex time dependence. We have attributed this dependence to the cell's orientational dynamics within the trap. We have also used experimentally measured phase function information to calculate the time dependence of the optical radiation pressure force on the trapped particle and show how it changes depending on the orientation of the particle. Relevance of these experiments to potential improvement in the sensitivity of label-free flow cytometry is discussed.</description><subject>Biochemistry</subject><subject>Cell Polarity - physiology</subject><subject>Cells, Cultured</subject><subject>Cellular biology</subject><subject>Diffusion</subject><subject>Elasticity</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Flow Cytometry - instrumentation</subject><subject>Flow Cytometry - methods</subject><subject>Fluorescence</subject><subject>Granulocytes - cytology</subject><subject>Humans</subject><subject>Leukocytes, Mononuclear - cytology</subject><subject>Melanoma - pathology</subject><subject>Micromanipulation - instrumentation</subject><subject>Micromanipulation - methods</subject><subject>Microscopy, Polarization - instrumentation</subject><subject>Microscopy, Polarization - methods</subject><subject>Optics and Photonics - instrumentation</subject><subject>Phantoms, 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The intensity of the scattered light was recorded over an angular range of 0.5–179.5° using an optical setup based on an elliptical mirror and rotating aperture. Experiments revealed that light-scattering diagrams from biological cells exhibit significant and complex time dependence. We have attributed this dependence to the cell's orientational dynamics within the trap. We have also used experimentally measured phase function information to calculate the time dependence of the optical radiation pressure force on the trapped particle and show how it changes depending on the orientation of the particle. Relevance of these experiments to potential improvement in the sensitivity of label-free flow cytometry is discussed.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15298932</pmid><doi>10.1529/biophysj.104.042135</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Cell Press Free Archives; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via ScienceDirect (Elsevier); PubMed Central |
| subjects | Biochemistry Cell Polarity - physiology Cells, Cultured Cellular biology Diffusion Elasticity Equipment Design Equipment Failure Analysis Flow Cytometry - instrumentation Flow Cytometry - methods Fluorescence Granulocytes - cytology Humans Leukocytes, Mononuclear - cytology Melanoma - pathology Micromanipulation - instrumentation Micromanipulation - methods Microscopy, Polarization - instrumentation Microscopy, Polarization - methods Optics and Photonics - instrumentation Phantoms, Imaging Physical Stimulation - instrumentation Physical Stimulation - methods Refractometry - instrumentation Refractometry - methods Reproducibility of Results Scatter diagrams Scattering, Radiation Sensitivity and Specificity Spectroscopy, Imaging, Other Techniques Spectrum analysis |
| title | Elastic Light Scattering from Single Cells: Orientational Dynamics in Optical Trap |
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