High Sensitivity of Stark-Shift Voltage-Sensing Dyes by One- or Two-Photon Excitation Near the Red Spectral Edge

Sensitivity spectra of Stark-shift voltage sensitive dyes, such as ANNINE-6, suggest the use of the extreme red edges of the excitation spectrum to achieve large fractional fluorescence changes with membrane voltage. This was tested in cultured HEK293 cells. Cells were illuminated with light at the...

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Veröffentlicht in:	Biophysical journal 2004-07, Vol.87 (1), p.631-639
Hauptverfasser:	Kuhn, Bernd, Fromherz, Peter, Denk, Winfried
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Atoms & subatomic particles Biochemistry Cells Cells, Cultured Dyes Electromagnetic Fields Fluorescence Fluorescent Dyes - chemistry Humans Membrane Potentials Membranes Photons Spectrometry, Fluorescence - methods Spectroscopy, Imaging, Other Techniques
Online-Zugang:	Volltext
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