Glutaraldehyde Modified Mica: A New Surface for Atomic Force Microscopy of Chromatin

We have found that mica surfaces functionalized with aminopropyltriethoxysilane and aldehydes bind chromatin strongly enough to permit stable and reliable solution imaging by atomic force microscopy. The method is highly reproducible, uses very small amounts of material, and is successful even with...

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Veröffentlicht in:	Biophysical journal 2002-12, Vol.83 (6), p.3619-3625
Hauptverfasser:	Wang, Hongda, Bash, Ralph, Yodh, Jiya G., Hager, Gordon L., Lohr, D., Lindsay, Stuart M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aluminum Silicates - chemistry Chemical bonds Chromatin - chemistry Chromatin - ultrastructure Glutaral - chemistry Methods Microscopy, Atomic Force - instrumentation Microscopy, Atomic Force - methods Nucleosomes - chemistry Nucleosomes - ultrastructure Quality Control Scientific imaging Sodium Chloride - chemistry Solutions - chemistry Surface Properties
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