Probing the Structure and Dynamics of a DNA Hairpin by Ultrafast Quenching and Fluorescence Depolarization

DNA hairpins have been investigated in which individual adenines were replaced by their fluorescent analog 2-aminopurine (2AP). The temperature dependence of the time evolution of polarized emission spectra was monitored with picosecond time resolution. Four isotropic decay components for each oligo...

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Veröffentlicht in:	Biophysical journal 2001-08, Vol.81 (2), p.1115-1126
Hauptverfasser:	Larsen, Olaf F.A., van Stokkum, Ivo H.M., Gobets, Bas, van Grondelle, Rienk, van Amerongen, Herbert
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biology Deoxyribonucleic acid DNA DNA - chemistry DNA - genetics DNA - metabolism Fluorescence Fluorescence Polarization Kinetics Nucleic Acid Conformation Nucleic Acid Denaturation Physics Spectrophotometry, Ultraviolet Temperature
Online-Zugang:	Volltext
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