Direct Imaging of Dehydrogenase Activity within Living Cells Using Enzyme-Dependent Fluorescence Recovery after Photobleaching (ED-FRAP)

Reduced nicotine adenine dinucleotide (NADH) is a key metabolite involved in cellular energy conversion and many redox reactions. We describe the use of confocal microscopy in conjunction with enzyme-dependent fluorescence recovery after photobleaching (ED-FRAP) of NADH as a topological assay of NAD...

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Veröffentlicht in:	Biophysical journal 2001-04, Vol.80 (4), p.2018-2028
Hauptverfasser:	Combs, C.A., Balaban, R.S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cellular biology Cyclic AMP - metabolism Dichloroacetic Acid - metabolism Enzymes Flavin-Adenine Dinucleotide - metabolism Fluorescence Glutamate Dehydrogenase - metabolism Image Processing, Computer-Assisted Microscopy, Confocal Mitochondria - metabolism Models, Chemical Myocardium - cytology NAD - metabolism Oxidoreductases - chemistry Perfusion Rabbits Spectrometry, Fluorescence Temperature Time Factors
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