Shear-Induced Assembly of λ-Phage DNA

Recombinant DNA technology, which is based on the assembly of DNA fragments, forms the backbone of biological and biomedical research. Here we demonstrate that a uniform shear flow can induce and control the assembly of λ-phage DNA molecules: increasing shear rates form integral DNA multimers of inc...

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Veröffentlicht in:	Biophysical journal 2000-09, Vol.79 (3), p.1530-1536
Hauptverfasser:	Haber, Charbel, Wirtz, Denis
Format:	Artikel
Sprache:	eng
Schlagworte:	Anatomy & physiology Bacteriophage lambda - genetics Calcium - pharmacology Cations, Divalent - pharmacology Deoxyribonucleic acid DNA DNA Ligases - metabolism DNA, Viral - chemistry DNA, Viral - drug effects DNA, Viral - metabolism Magnesium - pharmacology Models, Molecular Molecules Nucleic Acid Conformation Stress, Mechanical
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