Shear-Induced Assembly of λ-Phage DNA
Recombinant DNA technology, which is based on the assembly of DNA fragments, forms the backbone of biological and biomedical research. Here we demonstrate that a uniform shear flow can induce and control the assembly of λ-phage DNA molecules: increasing shear rates form integral DNA multimers of inc...
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| Veröffentlicht in: | Biophysical journal 2000-09, Vol.79 (3), p.1530-1536 |
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| creator | Haber, Charbel Wirtz, Denis |
| description | Recombinant DNA technology, which is based on the assembly of DNA fragments, forms the backbone of biological and biomedical research. Here we demonstrate that a uniform shear flow can induce and control the assembly of
λ-phage DNA molecules: increasing shear rates form integral DNA multimers of increasing molecular weight. Spontaneous assembly and grouping of end-blunted
λ-phage DNA molecules are negligible. It is suggested that shear-induced DNA assembly is caused by increasing the probability of contact between molecules and by stretching the molecules, which exposes the cohesive ends of the otherwise undeformed
λ-phage DNA molecules. We apply this principle to enhance the kinetics and extent of DNA concatenation in the presence of ligase. This novel approach to controlled DNA assembly could form the basis for improved approaches to gene-chip and recombinant DNA technologies and provide new insight into the rheology of associating polymers. |
| doi_str_mv | 10.1016/S0006-3495(00)76404-6 |
| format | Article |
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λ-phage DNA molecules: increasing shear rates form integral DNA multimers of increasing molecular weight. Spontaneous assembly and grouping of end-blunted
λ-phage DNA molecules are negligible. It is suggested that shear-induced DNA assembly is caused by increasing the probability of contact between molecules and by stretching the molecules, which exposes the cohesive ends of the otherwise undeformed
λ-phage DNA molecules. We apply this principle to enhance the kinetics and extent of DNA concatenation in the presence of ligase. This novel approach to controlled DNA assembly could form the basis for improved approaches to gene-chip and recombinant DNA technologies and provide new insight into the rheology of associating polymers.</description><identifier>ISSN: 0006-3495</identifier><identifier>EISSN: 1542-0086</identifier><identifier>DOI: 10.1016/S0006-3495(00)76404-6</identifier><identifier>PMID: 10969014</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Anatomy & physiology ; Bacteriophage lambda - genetics ; Calcium - pharmacology ; Cations, Divalent - pharmacology ; Deoxyribonucleic acid ; DNA ; DNA Ligases - metabolism ; DNA, Viral - chemistry ; DNA, Viral - drug effects ; DNA, Viral - metabolism ; Magnesium - pharmacology ; Models, Molecular ; Molecules ; Nucleic Acid Conformation ; Stress, Mechanical</subject><ispartof>Biophysical journal, 2000-09, Vol.79 (3), p.1530-1536</ispartof><rights>2000 The Biophysical Society</rights><rights>Copyright Biophysical Society Sep 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c521t-691a5bef71774136bfd49d88f1c4f3b6e95c50ccb4327af9685eb2759cb389703</citedby><cites>FETCH-LOGICAL-c521t-691a5bef71774136bfd49d88f1c4f3b6e95c50ccb4327af9685eb2759cb389703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1301046/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-3495(00)76404-6$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,3536,27903,27904,45974,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10969014$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Haber, Charbel</creatorcontrib><creatorcontrib>Wirtz, Denis</creatorcontrib><title>Shear-Induced Assembly of λ-Phage DNA</title><title>Biophysical journal</title><addtitle>Biophys J</addtitle><description>Recombinant DNA technology, which is based on the assembly of DNA fragments, forms the backbone of biological and biomedical research. Here we demonstrate that a uniform shear flow can induce and control the assembly of
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λ-phage DNA molecules are negligible. It is suggested that shear-induced DNA assembly is caused by increasing the probability of contact between molecules and by stretching the molecules, which exposes the cohesive ends of the otherwise undeformed
λ-phage DNA molecules. We apply this principle to enhance the kinetics and extent of DNA concatenation in the presence of ligase. 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genetics</topic><topic>Calcium - pharmacology</topic><topic>Cations, Divalent - pharmacology</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Ligases - metabolism</topic><topic>DNA, Viral - chemistry</topic><topic>DNA, Viral - drug effects</topic><topic>DNA, Viral - metabolism</topic><topic>Magnesium - pharmacology</topic><topic>Models, Molecular</topic><topic>Molecules</topic><topic>Nucleic Acid Conformation</topic><topic>Stress, Mechanical</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haber, Charbel</creatorcontrib><creatorcontrib>Wirtz, Denis</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biophysical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haber, Charbel</au><au>Wirtz, Denis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Shear-Induced Assembly of λ-Phage DNA</atitle><jtitle>Biophysical journal</jtitle><addtitle>Biophys J</addtitle><date>2000-09-01</date><risdate>2000</risdate><volume>79</volume><issue>3</issue><spage>1530</spage><epage>1536</epage><pages>1530-1536</pages><issn>0006-3495</issn><eissn>1542-0086</eissn><abstract>Recombinant DNA technology, which is based on the assembly of DNA fragments, forms the backbone of biological and biomedical research. Here we demonstrate that a uniform shear flow can induce and control the assembly of
λ-phage DNA molecules: increasing shear rates form integral DNA multimers of increasing molecular weight. Spontaneous assembly and grouping of end-blunted
λ-phage DNA molecules are negligible. It is suggested that shear-induced DNA assembly is caused by increasing the probability of contact between molecules and by stretching the molecules, which exposes the cohesive ends of the otherwise undeformed
λ-phage DNA molecules. We apply this principle to enhance the kinetics and extent of DNA concatenation in the presence of ligase. This novel approach to controlled DNA assembly could form the basis for improved approaches to gene-chip and recombinant DNA technologies and provide new insight into the rheology of associating polymers.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10969014</pmid><doi>10.1016/S0006-3495(00)76404-6</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Anatomy & physiology Bacteriophage lambda - genetics Calcium - pharmacology Cations, Divalent - pharmacology Deoxyribonucleic acid DNA DNA Ligases - metabolism DNA, Viral - chemistry DNA, Viral - drug effects DNA, Viral - metabolism Magnesium - pharmacology Models, Molecular Molecules Nucleic Acid Conformation Stress, Mechanical |
| title | Shear-Induced Assembly of λ-Phage DNA |
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