One- and Two-Photon Excited Fluorescence Lifetimes and Anisotropy Decays of Green Fluorescent Proteins

We have used one- (OPE) and two-photon (TPE) excitation with time-correlated single-photon counting techniques to determine time-resolved fluorescence intensity and anisotropy decays of the wild-type Green Fluorescent Protein (GFP) and two red-shifted mutants, S65T-GFP and RSGFP. WT-GFP and S65T-GFP...

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Veröffentlicht in:	Biophysical journal 2000-03, Vol.78 (3), p.1589-1598
Hauptverfasser:	Volkmer, Andreas, Subramaniam, Vinod, Birch, David J.S., Jovin, Thomas M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Substitution Fluorescence Fluorescence Polarization - methods Green Fluorescent Proteins Kinetics Least-Squares Analysis Luminescent Proteins - chemistry Luminescent Proteins - metabolism Molecular biology Mutagenesis, Site-Directed Photons Physics Proteins Recombinant Proteins - chemistry Recombinant Proteins - metabolism Spectrometry, Fluorescence - methods Time Factors
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