Nonlinear and Asymmetric Open Channel Characteristics of an Ion-Selective Porin in Planar Membranes
The open channel characteristics of the bacterial porin Omp32 from Comamonas acidovorans were investigated by means of conductance measurements in planar lipid bilayers of the Montal-Mueller type. Particularly at low salt conditions (≤30 mM KCl) Omp32 exhibited some unusual asymmetric and nonlinear...
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| Veröffentlicht in: | Biophysical journal 1998-09, Vol.75 (3), p.1255-1262 |
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| creator | Mathes, Anton Engelhardt, Harald |
| description | The open channel characteristics of the bacterial porin Omp32 from
Comamonas acidovorans were investigated by means of conductance measurements in planar lipid bilayers of the Montal-Mueller type. Particularly at low salt conditions (≤30
mM KCl) Omp32 exhibited some unusual asymmetric and nonlinear functional properties. Current-voltage relationship measurements showed that conductance depends on the orientation of porin molecules and is a nonlinear function of the applied membrane potential. Conductance also depends on the salt concentration in a manner not common to porins and the salt concentration modulates the nonlinearity of conductance-voltage relationships. Omp32 is strongly anion-selective. The nonlinear and asymmetric conductance of the open channel is a new observation in porins. |
| doi_str_mv | 10.1016/S0006-3495(98)74045-7 |
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mM KCl) Omp32 exhibited some unusual asymmetric and nonlinear functional properties. Current-voltage relationship measurements showed that conductance depends on the orientation of porin molecules and is a nonlinear function of the applied membrane potential. Conductance also depends on the salt concentration in a manner not common to porins and the salt concentration modulates the nonlinearity of conductance-voltage relationships. Omp32 is strongly anion-selective. The nonlinear and asymmetric conductance of the open channel is a new observation in porins.</description><identifier>ISSN: 0006-3495</identifier><identifier>EISSN: 1542-0086</identifier><identifier>DOI: 10.1016/S0006-3495(98)74045-7</identifier><identifier>PMID: 9726928</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Biophysical Phenomena ; Biophysics ; Electric Conductivity ; Gram-Negative Aerobic Rods and Cocci - metabolism ; Ion Channels - chemistry ; Ion Channels - metabolism ; Lipid Bilayers - chemistry ; Membrane Potentials ; Osmolar Concentration ; Porins - chemistry ; Porins - metabolism ; Potassium Chloride</subject><ispartof>Biophysical journal, 1998-09, Vol.75 (3), p.1255-1262</ispartof><rights>1998 The Biophysical Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-166b8f00676183cf4a16ab8d7ef950dd712b22d419bda74d4f9ed9222704ff4f3</citedby><cites>FETCH-LOGICAL-c462t-166b8f00676183cf4a16ab8d7ef950dd712b22d419bda74d4f9ed9222704ff4f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1299801/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-3495(98)74045-7$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,3548,27923,27924,45994,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9726928$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mathes, Anton</creatorcontrib><creatorcontrib>Engelhardt, Harald</creatorcontrib><title>Nonlinear and Asymmetric Open Channel Characteristics of an Ion-Selective Porin in Planar Membranes</title><title>Biophysical journal</title><addtitle>Biophys J</addtitle><description>The open channel characteristics of the bacterial porin Omp32 from
Comamonas acidovorans were investigated by means of conductance measurements in planar lipid bilayers of the Montal-Mueller type. Particularly at low salt conditions (≤30
mM KCl) Omp32 exhibited some unusual asymmetric and nonlinear functional properties. Current-voltage relationship measurements showed that conductance depends on the orientation of porin molecules and is a nonlinear function of the applied membrane potential. Conductance also depends on the salt concentration in a manner not common to porins and the salt concentration modulates the nonlinearity of conductance-voltage relationships. Omp32 is strongly anion-selective. The nonlinear and asymmetric conductance of the open channel is a new observation in porins.</description><subject>Biophysical Phenomena</subject><subject>Biophysics</subject><subject>Electric Conductivity</subject><subject>Gram-Negative Aerobic Rods and Cocci - metabolism</subject><subject>Ion Channels - chemistry</subject><subject>Ion Channels - metabolism</subject><subject>Lipid Bilayers - chemistry</subject><subject>Membrane Potentials</subject><subject>Osmolar Concentration</subject><subject>Porins - chemistry</subject><subject>Porins - metabolism</subject><subject>Potassium Chloride</subject><issn>0006-3495</issn><issn>1542-0086</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1rGzEQFaUldZ38hMCeQnPYVpK1-rgkBNOPQNoEkpyFVho1KruSI60N-feVY2PaU2FgDu_Nm5n3EDol-BPBhH--xxjzdsFU91HJc8Ew61rxBs1Ix2iLseRv0exAeY8-lPIbY0I7TI7QkRKUKypnyP5McQgRTG5MdM1VeRlHmHKwze0KYrN8MjHCsO3Z2AlyKFOwpUm-0pvrFNt7GMBOYQPNXcohNrXuBhOr3g8Y-2wilGP0zpuhwMm-z9Hj1y8Py-_tze236-XVTWsZp1NLOO-lrxcLTuTCemYIN710ArzqsHOC0J5Sx4jqnRHMMa_AKUqpwMx75hdzdLHTXa37EZyFOGUz6FUOo8kvOpmg_0VieNK_0kYTqpTEpAqc7QVyel5DmfQYioWh_gNpXbRYSE4F7yqx2xFtTqVk8IclBOttOvo1Hb21XiupX9Op43N0-veFh6l9HBW_3OFQbdoEyLrYANGCC7m6rF0K_9nwB17EoOw</recordid><startdate>19980901</startdate><enddate>19980901</enddate><creator>Mathes, Anton</creator><creator>Engelhardt, Harald</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19980901</creationdate><title>Nonlinear and Asymmetric Open Channel Characteristics of an Ion-Selective Porin in Planar Membranes</title><author>Mathes, Anton ; Engelhardt, Harald</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-166b8f00676183cf4a16ab8d7ef950dd712b22d419bda74d4f9ed9222704ff4f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Biophysical Phenomena</topic><topic>Biophysics</topic><topic>Electric Conductivity</topic><topic>Gram-Negative Aerobic Rods and Cocci - metabolism</topic><topic>Ion Channels - chemistry</topic><topic>Ion Channels - metabolism</topic><topic>Lipid Bilayers - chemistry</topic><topic>Membrane Potentials</topic><topic>Osmolar Concentration</topic><topic>Porins - chemistry</topic><topic>Porins - metabolism</topic><topic>Potassium Chloride</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mathes, Anton</creatorcontrib><creatorcontrib>Engelhardt, Harald</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biophysical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mathes, Anton</au><au>Engelhardt, Harald</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nonlinear and Asymmetric Open Channel Characteristics of an Ion-Selective Porin in Planar Membranes</atitle><jtitle>Biophysical journal</jtitle><addtitle>Biophys J</addtitle><date>1998-09-01</date><risdate>1998</risdate><volume>75</volume><issue>3</issue><spage>1255</spage><epage>1262</epage><pages>1255-1262</pages><issn>0006-3495</issn><eissn>1542-0086</eissn><abstract>The open channel characteristics of the bacterial porin Omp32 from
Comamonas acidovorans were investigated by means of conductance measurements in planar lipid bilayers of the Montal-Mueller type. Particularly at low salt conditions (≤30
mM KCl) Omp32 exhibited some unusual asymmetric and nonlinear functional properties. Current-voltage relationship measurements showed that conductance depends on the orientation of porin molecules and is a nonlinear function of the applied membrane potential. Conductance also depends on the salt concentration in a manner not common to porins and the salt concentration modulates the nonlinearity of conductance-voltage relationships. Omp32 is strongly anion-selective. The nonlinear and asymmetric conductance of the open channel is a new observation in porins.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9726928</pmid><doi>10.1016/S0006-3495(98)74045-7</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biophysical journal, 1998-09, Vol.75 (3), p.1255-1262 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Cell Press Free Archives; PubMed Central; EZB Electronic Journals Library |
| subjects | Biophysical Phenomena Biophysics Electric Conductivity Gram-Negative Aerobic Rods and Cocci - metabolism Ion Channels - chemistry Ion Channels - metabolism Lipid Bilayers - chemistry Membrane Potentials Osmolar Concentration Porins - chemistry Porins - metabolism Potassium Chloride |
| title | Nonlinear and Asymmetric Open Channel Characteristics of an Ion-Selective Porin in Planar Membranes |
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