Cooperative Activities of Hematopoietic Regulators Recruit RNA Polymerase II to a Tissue-Specific Chromatin Domain
The hematopoietic transcription factor GATA-1 regulates erythropoiesis and β-globin expression. Although consensus GATA-1 binding sites exist throughout the murine β-globin locus, we found that GATA-1 discriminates among these sites in vivo. Conditional expression of GATA-1 in GATA-1-null cells reca...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2002-09, Vol.99 (18), p.11760-11765 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Johnson, Kirby D. Grass, Jeffrey A. Boyer, Meghan E. Kiekhaefer, Carol M. Blobel, Gerd A. Weiss, Mitchell J. Bresnick, Emery H. |
| description | The hematopoietic transcription factor GATA-1 regulates erythropoiesis and β-globin expression. Although consensus GATA-1 binding sites exist throughout the murine β-globin locus, we found that GATA-1 discriminates among these sites in vivo. Conditional expression of GATA-1 in GATA-1-null cells recapitulated the occupancy pattern. GATA-1 induced RNA polymerase II (pol II) recruitment to subregions of the locus control region and to the β-globin promoters. The hematopoietic factor NF-E2 cooperated with GATA-1 to recruit pol II to the promoters. We propose that only when GATA-1 attracts pol II to the locus control region can pol II access the promoter in a NF-E2-dependent manner. |
| doi_str_mv | 10.1073/pnas.192285999 |
| format | Article |
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Although consensus GATA-1 binding sites exist throughout the murine β-globin locus, we found that GATA-1 discriminates among these sites in vivo. Conditional expression of GATA-1 in GATA-1-null cells recapitulated the occupancy pattern. GATA-1 induced RNA polymerase II (pol II) recruitment to subregions of the locus control region and to the β-globin promoters. The hematopoietic factor NF-E2 cooperated with GATA-1 to recruit pol II to the promoters. We propose that only when GATA-1 attracts pol II to the locus control region can pol II access the promoter in a NF-E2-dependent manner.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.192285999</identifier><identifier>PMID: 12193659</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Acetylation ; Animals ; Biological Sciences ; Bone Marrow - metabolism ; Cell lines ; Chromatin ; Chromatin - metabolism ; Crosslinking ; DNA ; DNA-Binding Proteins - metabolism ; Erythroid cells ; Erythroid-Specific DNA-Binding Factors ; GATA1 Transcription Factor ; Genetic loci ; Globins - genetics ; Mice ; NF-E2 Transcription Factor ; NF-E2 Transcription Factor, p45 Subunit ; Polymerase chain reaction ; Promoter Regions, Genetic ; RNA ; RNA Polymerase II - metabolism ; Transcription Factors - metabolism ; Tumor Cells, Cultured</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2002-09, Vol.99 (18), p.11760-11765</ispartof><rights>Copyright 1993-2002 National Academy of Sciences of the United States of America</rights><rights>Copyright © 2002, The National Academy of Sciences 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c560t-ea5cf9b7c7888e305d4e2c479e0e073620bda8827eed02d0e0c411407f36710e3</citedby><cites>FETCH-LOGICAL-c560t-ea5cf9b7c7888e305d4e2c479e0e073620bda8827eed02d0e0c411407f36710e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/99/18.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3073112$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3073112$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12193659$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Johnson, Kirby D.</creatorcontrib><creatorcontrib>Grass, Jeffrey A.</creatorcontrib><creatorcontrib>Boyer, Meghan E.</creatorcontrib><creatorcontrib>Kiekhaefer, Carol M.</creatorcontrib><creatorcontrib>Blobel, Gerd A.</creatorcontrib><creatorcontrib>Weiss, Mitchell J.</creatorcontrib><creatorcontrib>Bresnick, Emery H.</creatorcontrib><title>Cooperative Activities of Hematopoietic Regulators Recruit RNA Polymerase II to a Tissue-Specific Chromatin Domain</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The hematopoietic transcription factor GATA-1 regulates erythropoiesis and β-globin expression. Although consensus GATA-1 binding sites exist throughout the murine β-globin locus, we found that GATA-1 discriminates among these sites in vivo. Conditional expression of GATA-1 in GATA-1-null cells recapitulated the occupancy pattern. GATA-1 induced RNA polymerase II (pol II) recruitment to subregions of the locus control region and to the β-globin promoters. The hematopoietic factor NF-E2 cooperated with GATA-1 to recruit pol II to the promoters. We propose that only when GATA-1 attracts pol II to the locus control region can pol II access the promoter in a NF-E2-dependent manner.</description><subject>Acetylation</subject><subject>Animals</subject><subject>Biological Sciences</subject><subject>Bone Marrow - metabolism</subject><subject>Cell lines</subject><subject>Chromatin</subject><subject>Chromatin - metabolism</subject><subject>Crosslinking</subject><subject>DNA</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Erythroid cells</subject><subject>Erythroid-Specific DNA-Binding Factors</subject><subject>GATA1 Transcription Factor</subject><subject>Genetic loci</subject><subject>Globins - genetics</subject><subject>Mice</subject><subject>NF-E2 Transcription Factor</subject><subject>NF-E2 Transcription Factor, p45 Subunit</subject><subject>Polymerase chain reaction</subject><subject>Promoter Regions, Genetic</subject><subject>RNA</subject><subject>RNA Polymerase II - metabolism</subject><subject>Transcription Factors - metabolism</subject><subject>Tumor Cells, Cultured</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1vEzEQxS0EoqFw5YTAJ8Rlw9j7YfvAIQofjVQBKuVsOd7Z1tXuemt7K_rf4yghwAVOz_b83nhGj5DnDJYMRPl2Gk1cMsW5rJVSD8iCgWJFUyl4SBYAXBSy4tUJeRLjDQCoWsJjcsI4U2VTqwUJa-8nDCa5O6Qrm8Ulh5H6jp7hYJKfvMPkLL3Aq7nP9xDz0YbZJXrxeUW_-v5-yP6IdLOhyVNDL12MMxbfJrSuy871dfC5kxvp-6xufEoedaaP-Oygp-T7xw-X67Pi_MunzXp1Xti6gVSgqW2ntsIKKSWWULcVclsJhYB584bDtjVScoHYAm_zo60Yq0B0ZSMYYHlK3u37TvN2wNbimILp9RTcYMK99sbpvyuju9ZX_k4zrsqKZ__rgz_42xlj0oOLFvvejOjnqAWHuq5F81-QyUpCqeoMLvegDT7GgN1xGAZ6F6fexamPcWbDyz9X-I0f8svAqwOwM_4qK5X_1IyJBjLx5t-E7ua-T_gjZfTFHr2JOecjW-bBGOPlTwcmv0s</recordid><startdate>20020903</startdate><enddate>20020903</enddate><creator>Johnson, Kirby D.</creator><creator>Grass, Jeffrey A.</creator><creator>Boyer, Meghan E.</creator><creator>Kiekhaefer, Carol M.</creator><creator>Blobel, Gerd A.</creator><creator>Weiss, Mitchell J.</creator><creator>Bresnick, Emery H.</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><general>The National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20020903</creationdate><title>Cooperative Activities of Hematopoietic Regulators Recruit RNA Polymerase II to a Tissue-Specific Chromatin Domain</title><author>Johnson, Kirby D. ; Grass, Jeffrey A. ; Boyer, Meghan E. ; Kiekhaefer, Carol M. ; Blobel, Gerd A. ; Weiss, Mitchell J. ; Bresnick, Emery H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c560t-ea5cf9b7c7888e305d4e2c479e0e073620bda8827eed02d0e0c411407f36710e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Acetylation</topic><topic>Animals</topic><topic>Biological Sciences</topic><topic>Bone Marrow - metabolism</topic><topic>Cell lines</topic><topic>Chromatin</topic><topic>Chromatin - metabolism</topic><topic>Crosslinking</topic><topic>DNA</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Erythroid cells</topic><topic>Erythroid-Specific DNA-Binding Factors</topic><topic>GATA1 Transcription Factor</topic><topic>Genetic loci</topic><topic>Globins - genetics</topic><topic>Mice</topic><topic>NF-E2 Transcription Factor</topic><topic>NF-E2 Transcription Factor, p45 Subunit</topic><topic>Polymerase chain reaction</topic><topic>Promoter Regions, Genetic</topic><topic>RNA</topic><topic>RNA Polymerase II - metabolism</topic><topic>Transcription Factors - metabolism</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Johnson, Kirby D.</creatorcontrib><creatorcontrib>Grass, Jeffrey A.</creatorcontrib><creatorcontrib>Boyer, Meghan E.</creatorcontrib><creatorcontrib>Kiekhaefer, Carol M.</creatorcontrib><creatorcontrib>Blobel, Gerd A.</creatorcontrib><creatorcontrib>Weiss, Mitchell J.</creatorcontrib><creatorcontrib>Bresnick, Emery H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Johnson, Kirby D.</au><au>Grass, Jeffrey A.</au><au>Boyer, Meghan E.</au><au>Kiekhaefer, Carol M.</au><au>Blobel, Gerd A.</au><au>Weiss, Mitchell J.</au><au>Bresnick, Emery H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cooperative Activities of Hematopoietic Regulators Recruit RNA Polymerase II to a Tissue-Specific Chromatin Domain</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2002-09-03</date><risdate>2002</risdate><volume>99</volume><issue>18</issue><spage>11760</spage><epage>11765</epage><pages>11760-11765</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The hematopoietic transcription factor GATA-1 regulates erythropoiesis and β-globin expression. Although consensus GATA-1 binding sites exist throughout the murine β-globin locus, we found that GATA-1 discriminates among these sites in vivo. Conditional expression of GATA-1 in GATA-1-null cells recapitulated the occupancy pattern. GATA-1 induced RNA polymerase II (pol II) recruitment to subregions of the locus control region and to the β-globin promoters. The hematopoietic factor NF-E2 cooperated with GATA-1 to recruit pol II to the promoters. We propose that only when GATA-1 attracts pol II to the locus control region can pol II access the promoter in a NF-E2-dependent manner.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>12193659</pmid><doi>10.1073/pnas.192285999</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acetylation Animals Biological Sciences Bone Marrow - metabolism Cell lines Chromatin Chromatin - metabolism Crosslinking DNA DNA-Binding Proteins - metabolism Erythroid cells Erythroid-Specific DNA-Binding Factors GATA1 Transcription Factor Genetic loci Globins - genetics Mice NF-E2 Transcription Factor NF-E2 Transcription Factor, p45 Subunit Polymerase chain reaction Promoter Regions, Genetic RNA RNA Polymerase II - metabolism Transcription Factors - metabolism Tumor Cells, Cultured |
| title | Cooperative Activities of Hematopoietic Regulators Recruit RNA Polymerase II to a Tissue-Specific Chromatin Domain |
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