Fluorescence lifetime-based sensing in tissues: a computational study
We have numerically solved the photon diffusion equation to predict the distribution of light in a tissue model system with a uniform concentration of fluorophore. Our results show that time-dependent measurements of light propagation can be used to monitor the fluorescent lifetimes of a uniformly d...
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| Veröffentlicht in: | Biophysical journal 1995-04, Vol.68 (4), p.1574-1582 |
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| container_end_page | 1582 |
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| container_issue | 4 |
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| container_title | Biophysical journal |
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| creator | Hutchinson, C.L. Lakowicz, J.R. Sevick-Muraca, E.M. |
| description | We have numerically solved the photon diffusion equation to predict the distribution of light in a tissue model system with a uniform concentration of fluorophore. Our results show that time-dependent measurements of light propagation can be used to monitor the fluorescent lifetimes of a uniformly distributed fluorophore in tissues. With proper referencing, frequency-domain measurements of phase-shift, theta, may allow quantitation of fluorescent lifetimes, tau, independent of changes in the local absorption and scattering properties. These results point to a new approach for noninvasive diagnostic monitoring through quantitation of fluorescent lifetime, tau, when the lifetime of the fluorophore is comparable with photon migration times. |
| doi_str_mv | 10.1016/S0006-3495(95)80330-9 |
| format | Article |
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Our results show that time-dependent measurements of light propagation can be used to monitor the fluorescent lifetimes of a uniformly distributed fluorophore in tissues. With proper referencing, frequency-domain measurements of phase-shift, theta, may allow quantitation of fluorescent lifetimes, tau, independent of changes in the local absorption and scattering properties. 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Our results show that time-dependent measurements of light propagation can be used to monitor the fluorescent lifetimes of a uniformly distributed fluorophore in tissues. With proper referencing, frequency-domain measurements of phase-shift, theta, may allow quantitation of fluorescent lifetimes, tau, independent of changes in the local absorption and scattering properties. These results point to a new approach for noninvasive diagnostic monitoring through quantitation of fluorescent lifetime, tau, when the lifetime of the fluorophore is comparable with photon migration times.</description><subject>Biophysical Phenomena</subject><subject>Biophysics</subject><subject>Biosensing Techniques</subject><subject>Diffusion</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes - pharmacokinetics</subject><subject>Light</subject><subject>Models, Biological</subject><subject>Photons</subject><subject>Scattering, Radiation</subject><subject>Tissue Distribution</subject><issn>0006-3495</issn><issn>1542-0086</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LHEEQhptg0I3JT1iYk5jDxOqv-fCgyLImgpCD5tz0dNesLTPTa3fPgv8-s-6y6EkoqMP71ltVDyFzCr8o0OLiAQCKnItantfyZwWcQ15_ITMqBcsBquKIzA6WE_ItxmcAyiTQY3JcllUJgs_I8rYbfcBocDCYda7F5HrMGx3RZhGH6IZV5oYsuRhHjJeZzozv12PSyflBd1lMo339Tr62uov4Y99Pyb_b5ePiT37_9_fd4uY-N6JgKRe2tS1ayoUxiKKyhRFGY9NIy1jNmLRWSk6xtVpwQGmEtrLmjS1ZTQ23_JRc7XLXY9OjnY5OQXdqHVyvw6vy2qmPyuCe1MpvFGUVA8mmgLN9QPAv0z9J9W76vev0gH6Mqiy5gEJsjXJnNMHHGLA9LKGgtvzVG3-1haumeuOv6mlu_v7Cw9Qe-KRf73ScMG0cBhWN27K3LqBJynr3yYb_gPuYHg</recordid><startdate>19950401</startdate><enddate>19950401</enddate><creator>Hutchinson, C.L.</creator><creator>Lakowicz, J.R.</creator><creator>Sevick-Muraca, E.M.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19950401</creationdate><title>Fluorescence lifetime-based sensing in tissues: a computational study</title><author>Hutchinson, C.L. ; Lakowicz, J.R. ; Sevick-Muraca, E.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-4dfdfed134ccee48d6c4caebb5d229225dd5531efda430e5c4ad593bd7291c3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Biophysical Phenomena</topic><topic>Biophysics</topic><topic>Biosensing Techniques</topic><topic>Diffusion</topic><topic>Fluorescence</topic><topic>Fluorescent Dyes - pharmacokinetics</topic><topic>Light</topic><topic>Models, Biological</topic><topic>Photons</topic><topic>Scattering, Radiation</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hutchinson, C.L.</creatorcontrib><creatorcontrib>Lakowicz, J.R.</creatorcontrib><creatorcontrib>Sevick-Muraca, E.M.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biophysical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hutchinson, C.L.</au><au>Lakowicz, J.R.</au><au>Sevick-Muraca, E.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluorescence lifetime-based sensing in tissues: a computational study</atitle><jtitle>Biophysical journal</jtitle><addtitle>Biophys J</addtitle><date>1995-04-01</date><risdate>1995</risdate><volume>68</volume><issue>4</issue><spage>1574</spage><epage>1582</epage><pages>1574-1582</pages><issn>0006-3495</issn><eissn>1542-0086</eissn><abstract>We have numerically solved the photon diffusion equation to predict the distribution of light in a tissue model system with a uniform concentration of fluorophore. 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| identifier | ISSN: 0006-3495 |
| ispartof | Biophysical journal, 1995-04, Vol.68 (4), p.1574-1582 |
| issn | 0006-3495 1542-0086 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Cell Press Free Archives; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Biophysical Phenomena Biophysics Biosensing Techniques Diffusion Fluorescence Fluorescent Dyes - pharmacokinetics Light Models, Biological Photons Scattering, Radiation Tissue Distribution |
| title | Fluorescence lifetime-based sensing in tissues: a computational study |
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