Fast optical monitoring of microscopic excitation patterns in cardiac muscle
Many vital processes depend on the generation, changes, and conduction of cellular transmembrane potentials. Optical monitoring systems are well suited to detect such cellular electrical activities in networks of excitable cells and also tissues simultaneously at multiple sites. Here, an exceptional...
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| Veröffentlicht in: | Biophysical journal 1989-09, Vol.56 (3), p.623-629 |
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| container_title | Biophysical journal |
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| creator | Müller, W. Windisch, H. Tritthart, H.A. |
| description | Many vital processes depend on the generation, changes, and conduction of cellular transmembrane potentials. Optical monitoring systems are well suited to detect such cellular electrical activities in networks of excitable cells and also tissues simultaneously at multiple sites. Here, an exceptionally fast array system (16 x 16 photodiodes, up to 4,000,000 samples per second, 12-bit resolution) for imaging voltage-sensitive dye fluorescence, permitted real time measurements of excitation patterns at a microscopic size scale (256 pixels within an area of 1.8–8 mm2), in rat cardiac muscle in vitro. Results emphasize a recent hypothesis for cardiac impulse conduction, based on cardiac structural complexities, that is contradictory to all continuous cable theory models. |
| doi_str_mv | 10.1016/S0006-3495(89)82709-2 |
| format | Article |
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Results emphasize a recent hypothesis for cardiac impulse conduction, based on cardiac structural complexities, that is contradictory to all continuous cable theory models.</description><identifier>ISSN: 0006-3495</identifier><identifier>EISSN: 1542-0086</identifier><identifier>DOI: 10.1016/S0006-3495(89)82709-2</identifier><identifier>PMID: 2790142</identifier><identifier>CODEN: BIOJAU</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>Action Potentials ; Animals ; Biological and medical sciences ; Electric Stimulation ; Fluorescence ; Fundamental and applied biological sciences. 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Optical monitoring systems are well suited to detect such cellular electrical activities in networks of excitable cells and also tissues simultaneously at multiple sites. Here, an exceptionally fast array system (16 x 16 photodiodes, up to 4,000,000 samples per second, 12-bit resolution) for imaging voltage-sensitive dye fluorescence, permitted real time measurements of excitation patterns at a microscopic size scale (256 pixels within an area of 1.8–8 mm2), in rat cardiac muscle in vitro. Results emphasize a recent hypothesis for cardiac impulse conduction, based on cardiac structural complexities, that is contradictory to all continuous cable theory models.</description><subject>Action Potentials</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Electric Stimulation</subject><subject>Fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heart</subject><subject>Heart - physiology</subject><subject>In Vitro Techniques</subject><subject>Lasers</subject><subject>Microscopy</subject><subject>Muscles - physiology</subject><subject>Myocardium - cytology</subject><subject>Papillary Muscles - cytology</subject><subject>Papillary Muscles - physiology</subject><subject>Rats</subject><subject>Spectrometry, Fluorescence</subject><subject>Vertebrates: cardiovascular system</subject><issn>0006-3495</issn><issn>1542-0086</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkT1vFDEYhC0ECpfAT4jkBhSKJf7-aEAoIgHpJIpAbfne9QajXXuxfRH8--zlTgdUVC5m3vHoGYTOKXlLCVWXt4QQ1XFh5YWxbwzTxHbsCVpRKVhHiFFP0epoeY5Oa_1BCGWS0BN0wrQlVLAVWl_72nCeWwQ_4imn2HKJ6Q7nAU8RSq6Q5wg4_ILYfIs54dm3FkqqOCYMvvTRA562FcbwAj0b_FjDy8N7hr5df_x69albf7n5fPVh3YGUpnUK7NKLEa0GLUAMG029FQoIlcbbTTCD954PIHngUvdS6o0RkluuOA-W9PwMvdvnztvNFHoIqRU_urnEyZffLvvo_lVS_O7u8r2jzBBJ5RLw-hBQ8s9tqM1NsUIYR59C3lanLWNMa7YY5d64I1FLGI6fUOJ2M7jHGdyOsTPWPc7gdnfnfzc8Xh24L_qrg-7rAn4oPkGsf8KtEEorvfje731hwXkfQ3EVYkgQ-lgCNNfn-J8mD1I2pbU</recordid><startdate>19890901</startdate><enddate>19890901</enddate><creator>Müller, W.</creator><creator>Windisch, H.</creator><creator>Tritthart, H.A.</creator><general>Elsevier Inc</general><general>Biophysical Society</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19890901</creationdate><title>Fast optical monitoring of microscopic excitation patterns in cardiac muscle</title><author>Müller, W. ; Windisch, H. ; Tritthart, H.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c558t-6c90062076f74c4fb71a946c0158a9be8faaa3fc53e357d557b845393633e90d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Action Potentials</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Electric Stimulation</topic><topic>Fluorescence</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Heart</topic><topic>Heart - physiology</topic><topic>In Vitro Techniques</topic><topic>Lasers</topic><topic>Microscopy</topic><topic>Muscles - physiology</topic><topic>Myocardium - cytology</topic><topic>Papillary Muscles - cytology</topic><topic>Papillary Muscles - physiology</topic><topic>Rats</topic><topic>Spectrometry, Fluorescence</topic><topic>Vertebrates: cardiovascular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Müller, W.</creatorcontrib><creatorcontrib>Windisch, H.</creatorcontrib><creatorcontrib>Tritthart, H.A.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biophysical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Müller, W.</au><au>Windisch, H.</au><au>Tritthart, H.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fast optical monitoring of microscopic excitation patterns in cardiac muscle</atitle><jtitle>Biophysical journal</jtitle><addtitle>Biophys J</addtitle><date>1989-09-01</date><risdate>1989</risdate><volume>56</volume><issue>3</issue><spage>623</spage><epage>629</epage><pages>623-629</pages><issn>0006-3495</issn><eissn>1542-0086</eissn><coden>BIOJAU</coden><abstract>Many vital processes depend on the generation, changes, and conduction of cellular transmembrane potentials. 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| ispartof | Biophysical journal, 1989-09, Vol.56 (3), p.623-629 |
| issn | 0006-3495 1542-0086 |
| language | eng |
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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Action Potentials Animals Biological and medical sciences Electric Stimulation Fluorescence Fundamental and applied biological sciences. Psychology Heart Heart - physiology In Vitro Techniques Lasers Microscopy Muscles - physiology Myocardium - cytology Papillary Muscles - cytology Papillary Muscles - physiology Rats Spectrometry, Fluorescence Vertebrates: cardiovascular system |
| title | Fast optical monitoring of microscopic excitation patterns in cardiac muscle |
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