Proteins in cerebrospinal fluid and plasma of fetal sheep during development
1. The concentration of total protein in c.s.f. and plasma has been measured in fetal sheep of different gestational ages and in the adult. In c.s.f. it was highest (approximately 840 mg/100 ml.) in the youngest fetuses (35 days) and declined steeply by 60 days (260 mg/100 ml.). It decreased less ma...
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| Veröffentlicht in: | The Journal of physiology 1980-03, Vol.300 (1), p.441-455 |
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| creator | Dziegielewska, K. M. Evans, C. A. N. Fossan, G. Lorscheider, F. L. Malinowska, D. H. Møllgård, K. Reynolds, M. L. Saunders, N. R. Wilkinson, S. |
| description | 1. The concentration of total protein in c.s.f. and plasma has been measured in fetal sheep of different gestational ages
and in the adult. In c.s.f. it was highest (approximately 840 mg/100 ml.) in the youngest fetuses (35 days) and declined steeply
by 60 days (260 mg/100 ml.). It decreased less markedly in the last half of gestation to reach about 50 mg/100 ml. at 125
days which is twice the adult value. Protein concentration in plasma was lowest in the youngest fetuses and did not rise much
until the second half of gestation during which time it doubled. There was a further rather larger increase between the late
fetal (125 days) stage and the adult.
2. Albumin, fetuin, α-fetoprotein (AFP), transferrin and lipoprotein were identified in c.s.f. and plasma.
3. The concentrations of albumin, AFP and fetuin in c.s.f. and plasma at different gestational ages were measured using immunodiffusion
and radioimmuno-assays.
4. Albumin was the major protein in plasma at all ages studied (35-128 days gestation and adult). Its concentration increased
throughout gestation whereas that of fetuin was similar at all fetal ages and that of AFP declined markedly during the second
half of gestation. In the adult, fetuin was only about 0·1% of the total protein in plasma and AFP was not detectable.
5. In 35-40 day fetuses albumin, AFP and fetuin accounted for 90% of the total protein concentration in plasma and for about
70-80% of the total protein concentration of c.s.f. As gestation progressed the concentration of all three proteins in c.s.f.
declined. But the concentration of AFP and fetuin fell more rapidly and to a greater extent than that of albumin; neither
AFP nor fetuin could be detected in adult c.s.f.
6. The c.s.f.: Plasma ratio was above 50% for AFP and above 60% for fetuin at 35 days compared with about 25% for albumin
at the same fetal age. The c.s.f.: plasma ratios for all three proteins declined with increasing fetal age and were not significantly
different from each other by about mid gestation. |
| doi_str_mv | 10.1113/jphysiol.1980.sp013171 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1279364</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>75132019</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5331-211e051c5e5e5c9fd576be5da1c1dd624207f8bd5cec4a9dac127ec14217bd103</originalsourceid><addsrcrecordid>eNqNkU9v1DAQxS0EKtvCRwDlBL1k8cSJvbkgQcVfrUQP5Ww59mTjyomDnbTab4-jbCu4IOSDJb_fvBnPI-Q10C0AsHe3Y3eM1rst1Du6jSMFBgKekA2UvM6FqNlTsqG0KHImKnhOzmO8pQmidX1GzjhUVcnFhuyvg5_QDjGzQ6YxYBN8HO2gXNa62ZpMDSYbnYq9ynybtTglJXaIY2bmYIdDZvAOnR97HKYX5FmrXMSXp_uC_Pz86ebqa77_8eXb1Yd9rivGIC8AkFagK0xH162pBG-wMgo0GMOLsqCi3TWm0qhLVRuloRCooSxANAYouyDvV99xbno0OrUOyskx2F6Fo_TKyr-VwXby4O9k8qkZL5PBm5NB8L9mjJPsbdTonBrQz1GmjbGCQp3Ay3-CwPmuqBgXIqF8RXXaYAzYPs4DVC6RyYfI5BKZfIgsFb768zePZaeMkv5x1e-tw-N_usqb79fLA0uZl-XS5O1q0tlDd28DyrUsem1xOsrESZAL-RtXHrnc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1668253677</pqid></control><display><type>article</type><title>Proteins in cerebrospinal fluid and plasma of fetal sheep during development</title><source>MEDLINE</source><source>Wiley Blackwell Single Titles</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Dziegielewska, K. M. ; Evans, C. A. N. ; Fossan, G. ; Lorscheider, F. L. ; Malinowska, D. H. ; Møllgård, K. ; Reynolds, M. L. ; Saunders, N. R. ; Wilkinson, S.</creator><creatorcontrib>Dziegielewska, K. M. ; Evans, C. A. N. ; Fossan, G. ; Lorscheider, F. L. ; Malinowska, D. H. ; Møllgård, K. ; Reynolds, M. L. ; Saunders, N. R. ; Wilkinson, S.</creatorcontrib><description>1. The concentration of total protein in c.s.f. and plasma has been measured in fetal sheep of different gestational ages
and in the adult. In c.s.f. it was highest (approximately 840 mg/100 ml.) in the youngest fetuses (35 days) and declined steeply
by 60 days (260 mg/100 ml.). It decreased less markedly in the last half of gestation to reach about 50 mg/100 ml. at 125
days which is twice the adult value. Protein concentration in plasma was lowest in the youngest fetuses and did not rise much
until the second half of gestation during which time it doubled. There was a further rather larger increase between the late
fetal (125 days) stage and the adult.
2. Albumin, fetuin, α-fetoprotein (AFP), transferrin and lipoprotein were identified in c.s.f. and plasma.
3. The concentrations of albumin, AFP and fetuin in c.s.f. and plasma at different gestational ages were measured using immunodiffusion
and radioimmuno-assays.
4. Albumin was the major protein in plasma at all ages studied (35-128 days gestation and adult). Its concentration increased
throughout gestation whereas that of fetuin was similar at all fetal ages and that of AFP declined markedly during the second
half of gestation. In the adult, fetuin was only about 0·1% of the total protein in plasma and AFP was not detectable.
5. In 35-40 day fetuses albumin, AFP and fetuin accounted for 90% of the total protein concentration in plasma and for about
70-80% of the total protein concentration of c.s.f. As gestation progressed the concentration of all three proteins in c.s.f.
declined. But the concentration of AFP and fetuin fell more rapidly and to a greater extent than that of albumin; neither
AFP nor fetuin could be detected in adult c.s.f.
6. The c.s.f.: Plasma ratio was above 50% for AFP and above 60% for fetuin at 35 days compared with about 25% for albumin
at the same fetal age. The c.s.f.: plasma ratios for all three proteins declined with increasing fetal age and were not significantly
different from each other by about mid gestation.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.1980.sp013171</identifier><identifier>PMID: 6155467</identifier><language>eng</language><publisher>England: The Physiological Society</publisher><subject>Albumins - cerebrospinal fluid ; alpha-Fetoproteins - blood ; alpha-Fetoproteins - cerebrospinal fluid ; Animals ; Blood Proteins - analysis ; Cerebrospinal Fluid Proteins - analysis ; Female ; Fetal Blood - analysis ; Gestational Age ; Immunodiffusion ; Radioimmunoassay ; Serum Albumin - analysis ; Sheep - blood ; Sheep - cerebrospinal fluid ; Sheep - embryology</subject><ispartof>The Journal of physiology, 1980-03, Vol.300 (1), p.441-455</ispartof><rights>1980 The Physiological Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5331-211e051c5e5e5c9fd576be5da1c1dd624207f8bd5cec4a9dac127ec14217bd103</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1279364/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1279364/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,27924,27925,45574,45575,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6155467$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dziegielewska, K. M.</creatorcontrib><creatorcontrib>Evans, C. A. N.</creatorcontrib><creatorcontrib>Fossan, G.</creatorcontrib><creatorcontrib>Lorscheider, F. L.</creatorcontrib><creatorcontrib>Malinowska, D. H.</creatorcontrib><creatorcontrib>Møllgård, K.</creatorcontrib><creatorcontrib>Reynolds, M. L.</creatorcontrib><creatorcontrib>Saunders, N. R.</creatorcontrib><creatorcontrib>Wilkinson, S.</creatorcontrib><title>Proteins in cerebrospinal fluid and plasma of fetal sheep during development</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>1. The concentration of total protein in c.s.f. and plasma has been measured in fetal sheep of different gestational ages
and in the adult. In c.s.f. it was highest (approximately 840 mg/100 ml.) in the youngest fetuses (35 days) and declined steeply
by 60 days (260 mg/100 ml.). It decreased less markedly in the last half of gestation to reach about 50 mg/100 ml. at 125
days which is twice the adult value. Protein concentration in plasma was lowest in the youngest fetuses and did not rise much
until the second half of gestation during which time it doubled. There was a further rather larger increase between the late
fetal (125 days) stage and the adult.
2. Albumin, fetuin, α-fetoprotein (AFP), transferrin and lipoprotein were identified in c.s.f. and plasma.
3. The concentrations of albumin, AFP and fetuin in c.s.f. and plasma at different gestational ages were measured using immunodiffusion
and radioimmuno-assays.
4. Albumin was the major protein in plasma at all ages studied (35-128 days gestation and adult). Its concentration increased
throughout gestation whereas that of fetuin was similar at all fetal ages and that of AFP declined markedly during the second
half of gestation. In the adult, fetuin was only about 0·1% of the total protein in plasma and AFP was not detectable.
5. In 35-40 day fetuses albumin, AFP and fetuin accounted for 90% of the total protein concentration in plasma and for about
70-80% of the total protein concentration of c.s.f. As gestation progressed the concentration of all three proteins in c.s.f.
declined. But the concentration of AFP and fetuin fell more rapidly and to a greater extent than that of albumin; neither
AFP nor fetuin could be detected in adult c.s.f.
6. The c.s.f.: Plasma ratio was above 50% for AFP and above 60% for fetuin at 35 days compared with about 25% for albumin
at the same fetal age. The c.s.f.: plasma ratios for all three proteins declined with increasing fetal age and were not significantly
different from each other by about mid gestation.</description><subject>Albumins - cerebrospinal fluid</subject><subject>alpha-Fetoproteins - blood</subject><subject>alpha-Fetoproteins - cerebrospinal fluid</subject><subject>Animals</subject><subject>Blood Proteins - analysis</subject><subject>Cerebrospinal Fluid Proteins - analysis</subject><subject>Female</subject><subject>Fetal Blood - analysis</subject><subject>Gestational Age</subject><subject>Immunodiffusion</subject><subject>Radioimmunoassay</subject><subject>Serum Albumin - analysis</subject><subject>Sheep - blood</subject><subject>Sheep - cerebrospinal fluid</subject><subject>Sheep - embryology</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9v1DAQxS0EKtvCRwDlBL1k8cSJvbkgQcVfrUQP5Ww59mTjyomDnbTab4-jbCu4IOSDJb_fvBnPI-Q10C0AsHe3Y3eM1rst1Du6jSMFBgKekA2UvM6FqNlTsqG0KHImKnhOzmO8pQmidX1GzjhUVcnFhuyvg5_QDjGzQ6YxYBN8HO2gXNa62ZpMDSYbnYq9ynybtTglJXaIY2bmYIdDZvAOnR97HKYX5FmrXMSXp_uC_Pz86ebqa77_8eXb1Yd9rivGIC8AkFagK0xH162pBG-wMgo0GMOLsqCi3TWm0qhLVRuloRCooSxANAYouyDvV99xbno0OrUOyskx2F6Fo_TKyr-VwXby4O9k8qkZL5PBm5NB8L9mjJPsbdTonBrQz1GmjbGCQp3Ay3-CwPmuqBgXIqF8RXXaYAzYPs4DVC6RyYfI5BKZfIgsFb768zePZaeMkv5x1e-tw-N_usqb79fLA0uZl-XS5O1q0tlDd28DyrUsem1xOsrESZAL-RtXHrnc</recordid><startdate>19800301</startdate><enddate>19800301</enddate><creator>Dziegielewska, K. M.</creator><creator>Evans, C. A. N.</creator><creator>Fossan, G.</creator><creator>Lorscheider, F. L.</creator><creator>Malinowska, D. H.</creator><creator>Møllgård, K.</creator><creator>Reynolds, M. L.</creator><creator>Saunders, N. R.</creator><creator>Wilkinson, S.</creator><general>The Physiological Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19800301</creationdate><title>Proteins in cerebrospinal fluid and plasma of fetal sheep during development</title><author>Dziegielewska, K. M. ; Evans, C. A. N. ; Fossan, G. ; Lorscheider, F. L. ; Malinowska, D. H. ; Møllgård, K. ; Reynolds, M. L. ; Saunders, N. R. ; Wilkinson, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5331-211e051c5e5e5c9fd576be5da1c1dd624207f8bd5cec4a9dac127ec14217bd103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Albumins - cerebrospinal fluid</topic><topic>alpha-Fetoproteins - blood</topic><topic>alpha-Fetoproteins - cerebrospinal fluid</topic><topic>Animals</topic><topic>Blood Proteins - analysis</topic><topic>Cerebrospinal Fluid Proteins - analysis</topic><topic>Female</topic><topic>Fetal Blood - analysis</topic><topic>Gestational Age</topic><topic>Immunodiffusion</topic><topic>Radioimmunoassay</topic><topic>Serum Albumin - analysis</topic><topic>Sheep - blood</topic><topic>Sheep - cerebrospinal fluid</topic><topic>Sheep - embryology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dziegielewska, K. M.</creatorcontrib><creatorcontrib>Evans, C. A. N.</creatorcontrib><creatorcontrib>Fossan, G.</creatorcontrib><creatorcontrib>Lorscheider, F. L.</creatorcontrib><creatorcontrib>Malinowska, D. H.</creatorcontrib><creatorcontrib>Møllgård, K.</creatorcontrib><creatorcontrib>Reynolds, M. L.</creatorcontrib><creatorcontrib>Saunders, N. R.</creatorcontrib><creatorcontrib>Wilkinson, S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dziegielewska, K. M.</au><au>Evans, C. A. N.</au><au>Fossan, G.</au><au>Lorscheider, F. L.</au><au>Malinowska, D. H.</au><au>Møllgård, K.</au><au>Reynolds, M. L.</au><au>Saunders, N. R.</au><au>Wilkinson, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteins in cerebrospinal fluid and plasma of fetal sheep during development</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1980-03-01</date><risdate>1980</risdate><volume>300</volume><issue>1</issue><spage>441</spage><epage>455</epage><pages>441-455</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><abstract>1. The concentration of total protein in c.s.f. and plasma has been measured in fetal sheep of different gestational ages
and in the adult. In c.s.f. it was highest (approximately 840 mg/100 ml.) in the youngest fetuses (35 days) and declined steeply
by 60 days (260 mg/100 ml.). It decreased less markedly in the last half of gestation to reach about 50 mg/100 ml. at 125
days which is twice the adult value. Protein concentration in plasma was lowest in the youngest fetuses and did not rise much
until the second half of gestation during which time it doubled. There was a further rather larger increase between the late
fetal (125 days) stage and the adult.
2. Albumin, fetuin, α-fetoprotein (AFP), transferrin and lipoprotein were identified in c.s.f. and plasma.
3. The concentrations of albumin, AFP and fetuin in c.s.f. and plasma at different gestational ages were measured using immunodiffusion
and radioimmuno-assays.
4. Albumin was the major protein in plasma at all ages studied (35-128 days gestation and adult). Its concentration increased
throughout gestation whereas that of fetuin was similar at all fetal ages and that of AFP declined markedly during the second
half of gestation. In the adult, fetuin was only about 0·1% of the total protein in plasma and AFP was not detectable.
5. In 35-40 day fetuses albumin, AFP and fetuin accounted for 90% of the total protein concentration in plasma and for about
70-80% of the total protein concentration of c.s.f. As gestation progressed the concentration of all three proteins in c.s.f.
declined. But the concentration of AFP and fetuin fell more rapidly and to a greater extent than that of albumin; neither
AFP nor fetuin could be detected in adult c.s.f.
6. The c.s.f.: Plasma ratio was above 50% for AFP and above 60% for fetuin at 35 days compared with about 25% for albumin
at the same fetal age. The c.s.f.: plasma ratios for all three proteins declined with increasing fetal age and were not significantly
different from each other by about mid gestation.</abstract><cop>England</cop><pub>The Physiological Society</pub><pmid>6155467</pmid><doi>10.1113/jphysiol.1980.sp013171</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3751 |
| ispartof | The Journal of physiology, 1980-03, Vol.300 (1), p.441-455 |
| issn | 0022-3751 1469-7793 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1279364 |
| source | MEDLINE; Wiley Blackwell Single Titles; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Albumins - cerebrospinal fluid alpha-Fetoproteins - blood alpha-Fetoproteins - cerebrospinal fluid Animals Blood Proteins - analysis Cerebrospinal Fluid Proteins - analysis Female Fetal Blood - analysis Gestational Age Immunodiffusion Radioimmunoassay Serum Albumin - analysis Sheep - blood Sheep - cerebrospinal fluid Sheep - embryology |
| title | Proteins in cerebrospinal fluid and plasma of fetal sheep during development |
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