A comparison of effects evoked in guinea-pig taenia caecum by purine nucleotides and by `purinergic' nerve stimulation
1. Effects of adenosine and the adenosine nucleotides (ATP, ADP, AMP and CoA) on the guinea-pig taenia caecum were studied by measuring simultaneously the changes in membrane potential and in contractility induced by these agents using the sucrose-gap technique. 2. In the presence of carbachol (5 Ã...
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Veröffentlicht in: | The Journal of physiology 1980-02, Vol.299 (1), p.75-83 |
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description | 1. Effects of adenosine and the adenosine nucleotides (ATP, ADP, AMP and CoA) on the guinea-pig taenia caecum were studied
by measuring simultaneously the changes in membrane potential and in contractility induced by these agents using the sucrose-gap
technique.
2. In the presence of carbachol (5 Ã 10 -8 M ) the hyperpolarization and the relaxation induced by these agents was found to be closely correlated. Their different potencies
suggested an action on the P 2 type purinoceptor: ATP ⥠ADP ⥠AMP > CoA > AD.
3. The transmitter released endogenously in the presence of carbachol (5 Ã 10 -8 M ) by the non-adrenergic inhibitory (n.a.i.) nerves after half-maximal stimulation induced an inhibitory junction potential
(i.j.p.) which transiently counteracted the carbachol-induced depolarization. This i.j.p. was mimicked by ATP, ADP, and AMP
applied exogenously in concentrations of about 3 Ã 10 -5 M , by CoA (3 Ã 10 -4 M ) and by adenosine (3 Ã 10 -3 M ).
4. The results presented are in agreement with the hypothesis that the transmitter substance released by the n.a.i. nerves
is a purine nucleotide, which in the guinea-pig taenia caecum affects the smooth muscle cell membrane via the P 2 -purinoceptor. Of the putative transmitters studied, ATP, ADP and AMP seem the most likely. |
doi_str_mv | 10.1113/jphysiol.1980.sp013111 |
format | Article |
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by measuring simultaneously the changes in membrane potential and in contractility induced by these agents using the sucrose-gap
technique.
2. In the presence of carbachol (5 Ã 10 -8 M ) the hyperpolarization and the relaxation induced by these agents was found to be closely correlated. Their different potencies
suggested an action on the P 2 type purinoceptor: ATP ⥠ADP ⥠AMP > CoA > AD.
3. The transmitter released endogenously in the presence of carbachol (5 Ã 10 -8 M ) by the non-adrenergic inhibitory (n.a.i.) nerves after half-maximal stimulation induced an inhibitory junction potential
(i.j.p.) which transiently counteracted the carbachol-induced depolarization. This i.j.p. was mimicked by ATP, ADP, and AMP
applied exogenously in concentrations of about 3 Ã 10 -5 M , by CoA (3 Ã 10 -4 M ) and by adenosine (3 Ã 10 -3 M ).
4. The results presented are in agreement with the hypothesis that the transmitter substance released by the n.a.i. nerves
is a purine nucleotide, which in the guinea-pig taenia caecum affects the smooth muscle cell membrane via the P 2 -purinoceptor. Of the putative transmitters studied, ATP, ADP and AMP seem the most likely.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.1980.sp013111</identifier><identifier>PMID: 7381779</identifier><language>eng</language><publisher>England: The Physiological Society</publisher><subject>Adenine Nucleotides - pharmacology ; Adenosine - pharmacology ; Animals ; Carbachol - pharmacology ; Cecum - drug effects ; Cecum - physiology ; Female ; Guinea Pigs ; In Vitro Techniques ; Male ; Membrane Potentials - drug effects ; Muscle Contraction - drug effects ; Muscle, Smooth - drug effects ; Muscle, Smooth - physiology ; Taenia</subject><ispartof>The Journal of physiology, 1980-02, Vol.299 (1), p.75-83</ispartof><rights>1980 The Physiological Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4465-d2240cd70c8051b57e63a5144c3c3f8119dd6bef15a6540a0b2f410cd298bd013</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1279211/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1279211/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,725,778,782,883,1414,27911,27912,45561,45562,53778,53780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7381779$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jager, L. P.</creatorcontrib><creatorcontrib>Schevers, J. A. M.</creatorcontrib><title>A comparison of effects evoked in guinea-pig taenia caecum by purine nucleotides and by `purinergic' nerve stimulation</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>1. Effects of adenosine and the adenosine nucleotides (ATP, ADP, AMP and CoA) on the guinea-pig taenia caecum were studied
by measuring simultaneously the changes in membrane potential and in contractility induced by these agents using the sucrose-gap
technique.
2. In the presence of carbachol (5 Ã 10 -8 M ) the hyperpolarization and the relaxation induced by these agents was found to be closely correlated. Their different potencies
suggested an action on the P 2 type purinoceptor: ATP ⥠ADP ⥠AMP > CoA > AD.
3. The transmitter released endogenously in the presence of carbachol (5 Ã 10 -8 M ) by the non-adrenergic inhibitory (n.a.i.) nerves after half-maximal stimulation induced an inhibitory junction potential
(i.j.p.) which transiently counteracted the carbachol-induced depolarization. This i.j.p. was mimicked by ATP, ADP, and AMP
applied exogenously in concentrations of about 3 Ã 10 -5 M , by CoA (3 Ã 10 -4 M ) and by adenosine (3 Ã 10 -3 M ).
4. The results presented are in agreement with the hypothesis that the transmitter substance released by the n.a.i. nerves
is a purine nucleotide, which in the guinea-pig taenia caecum affects the smooth muscle cell membrane via the P 2 -purinoceptor. Of the putative transmitters studied, ATP, ADP and AMP seem the most likely.</description><subject>Adenine Nucleotides - pharmacology</subject><subject>Adenosine - pharmacology</subject><subject>Animals</subject><subject>Carbachol - pharmacology</subject><subject>Cecum - drug effects</subject><subject>Cecum - physiology</subject><subject>Female</subject><subject>Guinea Pigs</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Membrane Potentials - drug effects</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle, Smooth - drug effects</subject><subject>Muscle, Smooth - physiology</subject><subject>Taenia</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUU1v1DAQtRCobAs_AeQTcMnisfOxviCtKiigSnAoZ-M4k6xLEgc72Sr_HkfZFrggTiPN-9C8eYS8BLYFAPH2djjMwbp2C3LHtmFgIOL-EdlAmsukKKR4TDaMcZ6IIoOn5DyEWxZJTMozclaIHUTOhhz31Lhu0N4G11NXU6xrNGOgeHQ_sKK2p81ke9TJYBs6auytpkajmTpaznSYfARpP5kW3WgrDFT31YJ8XyHfWPOaxnlEGkbbTa0ereufkSe1bgM-P80L8u3D-5vLj8n1l6tPl_vrxKRpniUV5ykzVcHMjmVQZgXmQmeQpkYYUe8AZFXlJdaQ6TxLmWYlr1OICi53ZRXTXpB3q-8wlR1WBvvR61YN3nbaz8ppq_5GentQjTsq4IXksBi8Ohl493PCMKrOBoNtq3t0U1DxtyJjvIjEN_8kQjwwz2XKRaTmK9V4F4LH-uEeYGopV92Xq5Zy1X25UfjizzQPslObEd-v-J1tcf5PV3Xz-euy4FJCkf0OfLDN4c56VKsqOGNxnFWkKYixxS89QMgO</recordid><startdate>19800201</startdate><enddate>19800201</enddate><creator>Jager, L. P.</creator><creator>Schevers, J. A. M.</creator><general>The Physiological Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19800201</creationdate><title>A comparison of effects evoked in guinea-pig taenia caecum by purine nucleotides and by `purinergic' nerve stimulation</title><author>Jager, L. P. ; Schevers, J. A. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4465-d2240cd70c8051b57e63a5144c3c3f8119dd6bef15a6540a0b2f410cd298bd013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Adenine Nucleotides - pharmacology</topic><topic>Adenosine - pharmacology</topic><topic>Animals</topic><topic>Carbachol - pharmacology</topic><topic>Cecum - drug effects</topic><topic>Cecum - physiology</topic><topic>Female</topic><topic>Guinea Pigs</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Membrane Potentials - drug effects</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle, Smooth - drug effects</topic><topic>Muscle, Smooth - physiology</topic><topic>Taenia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jager, L. P.</creatorcontrib><creatorcontrib>Schevers, J. A. M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jager, L. P.</au><au>Schevers, J. A. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparison of effects evoked in guinea-pig taenia caecum by purine nucleotides and by `purinergic' nerve stimulation</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1980-02-01</date><risdate>1980</risdate><volume>299</volume><issue>1</issue><spage>75</spage><epage>83</epage><pages>75-83</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><abstract>1. Effects of adenosine and the adenosine nucleotides (ATP, ADP, AMP and CoA) on the guinea-pig taenia caecum were studied
by measuring simultaneously the changes in membrane potential and in contractility induced by these agents using the sucrose-gap
technique.
2. In the presence of carbachol (5 Ã 10 -8 M ) the hyperpolarization and the relaxation induced by these agents was found to be closely correlated. Their different potencies
suggested an action on the P 2 type purinoceptor: ATP ⥠ADP ⥠AMP > CoA > AD.
3. The transmitter released endogenously in the presence of carbachol (5 Ã 10 -8 M ) by the non-adrenergic inhibitory (n.a.i.) nerves after half-maximal stimulation induced an inhibitory junction potential
(i.j.p.) which transiently counteracted the carbachol-induced depolarization. This i.j.p. was mimicked by ATP, ADP, and AMP
applied exogenously in concentrations of about 3 Ã 10 -5 M , by CoA (3 Ã 10 -4 M ) and by adenosine (3 Ã 10 -3 M ).
4. The results presented are in agreement with the hypothesis that the transmitter substance released by the n.a.i. nerves
is a purine nucleotide, which in the guinea-pig taenia caecum affects the smooth muscle cell membrane via the P 2 -purinoceptor. Of the putative transmitters studied, ATP, ADP and AMP seem the most likely.</abstract><cop>England</cop><pub>The Physiological Society</pub><pmid>7381779</pmid><doi>10.1113/jphysiol.1980.sp013111</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
subjects | Adenine Nucleotides - pharmacology Adenosine - pharmacology Animals Carbachol - pharmacology Cecum - drug effects Cecum - physiology Female Guinea Pigs In Vitro Techniques Male Membrane Potentials - drug effects Muscle Contraction - drug effects Muscle, Smooth - drug effects Muscle, Smooth - physiology Taenia |
title | A comparison of effects evoked in guinea-pig taenia caecum by purine nucleotides and by `purinergic' nerve stimulation |
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