The reflexion coefficient as a measure of transepithelial permeability in the isolated rabbit pancreas
1. The reflexion coefficients of a number of non-electrolytes and electrolytes have been determined in the isolated rabbit pancreas. 2. The reflexion coefficients of the following non-electrolytes were: urea, -0.02; glycerol, 0.06; erythritol, 0.11; sorbitol, 0.41; mannitol, 0.42; arabinose, 0.72; x...
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| Veröffentlicht in: | The Journal of physiology 1980-12, Vol.309 (1), p.547-555 |
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| creator | S L Bonting J J De Pont A M Fleuren-Jakobs J W Jansen |
| description | 1. The reflexion coefficients of a number of non-electrolytes and electrolytes have been determined in the isolated rabbit
pancreas. 2. The reflexion coefficients of the following non-electrolytes were: urea, -0.02; glycerol, 0.06; erythritol, 0.11;
sorbitol, 0.41; mannitol, 0.42; arabinose, 0.72; xylose, 0.74, assuming a value of 1.00 for sucrose. 3. These values are equal
within the experimental error to values previously obtained with a tracer technique for the same preparation, but they are
significantly lower than those reported by other investigators for the isolated perfused cat pancreas. 4. Addition of 100
mM-sucrose to the bathing medium resulted in proportionally increased Na+ and K+ concentrations in the secreted fluid. The
secreted fluid remained isotonic with the bathing medium under all circumstances. 5. Addition of 10(-5) M-carbachol to the
bathing medium led to a reduction in the reflexion coefficient of sucrose from 1 to 0.85, but only when 25 mM-sucrose was
used. 6. The reflexion coefficients of electrolytes were: NaCl, 0.50; KCl, 0.51; NaHCO3, 0.52 and choline chloride, 1.02.
7. It is concluded that the isolated rabbit pancreas is highly permeable, both to electrolytes and to small non-electrolytes,
probably being more leaky than any other epithelium studied so far. |
| doi_str_mv | 10.1113/jphysiol.1980.sp013526 |
| format | Article |
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pancreas. 2. The reflexion coefficients of the following non-electrolytes were: urea, -0.02; glycerol, 0.06; erythritol, 0.11;
sorbitol, 0.41; mannitol, 0.42; arabinose, 0.72; xylose, 0.74, assuming a value of 1.00 for sucrose. 3. These values are equal
within the experimental error to values previously obtained with a tracer technique for the same preparation, but they are
significantly lower than those reported by other investigators for the isolated perfused cat pancreas. 4. Addition of 100
mM-sucrose to the bathing medium resulted in proportionally increased Na+ and K+ concentrations in the secreted fluid. The
secreted fluid remained isotonic with the bathing medium under all circumstances. 5. Addition of 10(-5) M-carbachol to the
bathing medium led to a reduction in the reflexion coefficient of sucrose from 1 to 0.85, but only when 25 mM-sucrose was
used. 6. The reflexion coefficients of electrolytes were: NaCl, 0.50; KCl, 0.51; NaHCO3, 0.52 and choline chloride, 1.02.
7. It is concluded that the isolated rabbit pancreas is highly permeable, both to electrolytes and to small non-electrolytes,
probably being more leaky than any other epithelium studied so far.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.1980.sp013526</identifier><identifier>PMID: 6788940</identifier><language>eng</language><publisher>England: The Physiological Society</publisher><subject>Animals ; Arabinose - metabolism ; Carbachol - pharmacology ; Cell Membrane Permeability ; Electrolytes - metabolism ; Epithelium - metabolism ; Female ; In Vitro Techniques ; Male ; Mannitol - metabolism ; Pancreas - metabolism ; Pancreatic Juice - metabolism ; Rabbits ; Secretory Rate - drug effects ; Sucrose - metabolism ; Sucrose - pharmacology ; Urea - metabolism</subject><ispartof>The Journal of physiology, 1980-12, Vol.309 (1), p.547-555</ispartof><rights>1980 The Physiological Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4157-a37bc9e098071f3797a53f23849d3d95f0abad7e30da8934e826672d5e749cd53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1274602/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1274602/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,27924,27925,45574,45575,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6788940$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>S L Bonting</creatorcontrib><creatorcontrib>J J De Pont</creatorcontrib><creatorcontrib>A M Fleuren-Jakobs</creatorcontrib><creatorcontrib>J W Jansen</creatorcontrib><title>The reflexion coefficient as a measure of transepithelial permeability in the isolated rabbit pancreas</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>1. The reflexion coefficients of a number of non-electrolytes and electrolytes have been determined in the isolated rabbit
pancreas. 2. The reflexion coefficients of the following non-electrolytes were: urea, -0.02; glycerol, 0.06; erythritol, 0.11;
sorbitol, 0.41; mannitol, 0.42; arabinose, 0.72; xylose, 0.74, assuming a value of 1.00 for sucrose. 3. These values are equal
within the experimental error to values previously obtained with a tracer technique for the same preparation, but they are
significantly lower than those reported by other investigators for the isolated perfused cat pancreas. 4. Addition of 100
mM-sucrose to the bathing medium resulted in proportionally increased Na+ and K+ concentrations in the secreted fluid. The
secreted fluid remained isotonic with the bathing medium under all circumstances. 5. Addition of 10(-5) M-carbachol to the
bathing medium led to a reduction in the reflexion coefficient of sucrose from 1 to 0.85, but only when 25 mM-sucrose was
used. 6. The reflexion coefficients of electrolytes were: NaCl, 0.50; KCl, 0.51; NaHCO3, 0.52 and choline chloride, 1.02.
7. It is concluded that the isolated rabbit pancreas is highly permeable, both to electrolytes and to small non-electrolytes,
probably being more leaky than any other epithelium studied so far.</description><subject>Animals</subject><subject>Arabinose - metabolism</subject><subject>Carbachol - pharmacology</subject><subject>Cell Membrane Permeability</subject><subject>Electrolytes - metabolism</subject><subject>Epithelium - metabolism</subject><subject>Female</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Mannitol - metabolism</subject><subject>Pancreas - metabolism</subject><subject>Pancreatic Juice - metabolism</subject><subject>Rabbits</subject><subject>Secretory Rate - drug effects</subject><subject>Sucrose - metabolism</subject><subject>Sucrose - pharmacology</subject><subject>Urea - metabolism</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EKkPhEUBewSqDHcdxvEGiFeVHlWAxrC0nuW5u5YmDnaHk7etRphXsWFm655zPvj6EvOFsyzkX72-nYUkY_Jbrhm3TxLiQZf2EbHhV60IpLZ6SDWNlWQgl-XPyIqVblk1M6zNyVqum0RXbELcbgEZwHv5gGGkXwDnsEMaZ2kQt3YNNhwg0ODpHOyaYcB7Ao_V0gpjVFj3OC8WR5jnFFLydoafRti3OdLJjFzPiJXnmrE_w6nSek59Xn3aXX4rr75-_Xn68LrqKS1VYodpOA8srKe6E0spK4UrRVLoXvZaO2db2CgTrbaNFBU1Z16rsJahKd70U5-TDyp0O7R76Lu8RrTdTxL2NiwkWzb_KiIO5Cb8NL1VVszID3p4AMfw6QJrNHlMH3tsRwiEZJaXQnNfZWK_GLoaU8g8-XsKZOTZkHhoyx4bMQ0M5-PrvJz7GTpVk_WLV79DD8p9Us_v24zjI9XJZqQx5t0IGvBnuMIJZYynkbufFZJ_h5ui8B92mtVk</recordid><startdate>19801201</startdate><enddate>19801201</enddate><creator>S L Bonting</creator><creator>J J De Pont</creator><creator>A M Fleuren-Jakobs</creator><creator>J W Jansen</creator><general>The Physiological Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19801201</creationdate><title>The reflexion coefficient as a measure of transepithelial permeability in the isolated rabbit pancreas</title><author>S L Bonting ; J J De Pont ; A M Fleuren-Jakobs ; J W Jansen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4157-a37bc9e098071f3797a53f23849d3d95f0abad7e30da8934e826672d5e749cd53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Animals</topic><topic>Arabinose - metabolism</topic><topic>Carbachol - pharmacology</topic><topic>Cell Membrane Permeability</topic><topic>Electrolytes - metabolism</topic><topic>Epithelium - metabolism</topic><topic>Female</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Mannitol - metabolism</topic><topic>Pancreas - metabolism</topic><topic>Pancreatic Juice - metabolism</topic><topic>Rabbits</topic><topic>Secretory Rate - drug effects</topic><topic>Sucrose - metabolism</topic><topic>Sucrose - pharmacology</topic><topic>Urea - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>S L Bonting</creatorcontrib><creatorcontrib>J J De Pont</creatorcontrib><creatorcontrib>A M Fleuren-Jakobs</creatorcontrib><creatorcontrib>J W Jansen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>S L Bonting</au><au>J J De Pont</au><au>A M Fleuren-Jakobs</au><au>J W Jansen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The reflexion coefficient as a measure of transepithelial permeability in the isolated rabbit pancreas</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1980-12-01</date><risdate>1980</risdate><volume>309</volume><issue>1</issue><spage>547</spage><epage>555</epage><pages>547-555</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><abstract>1. The reflexion coefficients of a number of non-electrolytes and electrolytes have been determined in the isolated rabbit
pancreas. 2. The reflexion coefficients of the following non-electrolytes were: urea, -0.02; glycerol, 0.06; erythritol, 0.11;
sorbitol, 0.41; mannitol, 0.42; arabinose, 0.72; xylose, 0.74, assuming a value of 1.00 for sucrose. 3. These values are equal
within the experimental error to values previously obtained with a tracer technique for the same preparation, but they are
significantly lower than those reported by other investigators for the isolated perfused cat pancreas. 4. Addition of 100
mM-sucrose to the bathing medium resulted in proportionally increased Na+ and K+ concentrations in the secreted fluid. The
secreted fluid remained isotonic with the bathing medium under all circumstances. 5. Addition of 10(-5) M-carbachol to the
bathing medium led to a reduction in the reflexion coefficient of sucrose from 1 to 0.85, but only when 25 mM-sucrose was
used. 6. The reflexion coefficients of electrolytes were: NaCl, 0.50; KCl, 0.51; NaHCO3, 0.52 and choline chloride, 1.02.
7. It is concluded that the isolated rabbit pancreas is highly permeable, both to electrolytes and to small non-electrolytes,
probably being more leaky than any other epithelium studied so far.</abstract><cop>England</cop><pub>The Physiological Society</pub><pmid>6788940</pmid><doi>10.1113/jphysiol.1980.sp013526</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of physiology, 1980-12, Vol.309 (1), p.547-555 |
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| language | eng |
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| source | MEDLINE; Free E-Journal (出版社公開部分のみ）; Wiley Online Library All Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Arabinose - metabolism Carbachol - pharmacology Cell Membrane Permeability Electrolytes - metabolism Epithelium - metabolism Female In Vitro Techniques Male Mannitol - metabolism Pancreas - metabolism Pancreatic Juice - metabolism Rabbits Secretory Rate - drug effects Sucrose - metabolism Sucrose - pharmacology Urea - metabolism |
| title | The reflexion coefficient as a measure of transepithelial permeability in the isolated rabbit pancreas |
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