Clearing-factor lipase in adipose tissue. A medium in which the enzyme activity of tissue from starved rats increases in vitro

1. When epididymal fat bodies from starved rats are incubated for 3.5hr. at 37 degrees in a defined medium in vitro the total clearing-factor lipase activity rises to approximately twice its initial value. 2. During the incubation period part of the tissue clearing-factor lipase activity appears in...

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Veröffentlicht in:	Biochemical Journal 1966-06, Vol.99 (3), p.640-647
Hauptverfasser:	Salaman, M R, Robinson, D S
Format:	Artikel
Sprache:	eng
Schlagworte:	Adipose Tissue - enzymology Amino Acids - metabolism Animals Bicarbonates - pharmacology Culture Media Glucose - metabolism Heparin - pharmacology Insulin - pharmacology Lipid Metabolism Lipoprotein Lipase - metabolism Lipoproteins - blood Rats Starvation
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container_title	Biochemical Journal
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description	1. When epididymal fat bodies from starved rats are incubated for 3.5hr. at 37 degrees in a defined medium in vitro the total clearing-factor lipase activity rises to approximately twice its initial value. 2. During the incubation period part of the tissue clearing-factor lipase activity appears in the medium. 3. Heparin, glucose, insulin, and HCO(3) (-) and K(+) ions are shown to be important medium constituents.
doi_str_mv	10.1042/bj0990640
format	Article
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A medium in which the enzyme activity of tissue from starved rats increases in vitro</title><title>Biochemical Journal</title><addtitle>Biochem J</addtitle><description>1. When epididymal fat bodies from starved rats are incubated for 3.5hr. at 37 degrees in a defined medium in vitro the total clearing-factor lipase activity rises to approximately twice its initial value. 2. During the incubation period part of the tissue clearing-factor lipase activity appears in the medium. 3. Heparin, glucose, insulin, and HCO(3) (-) and K(+) ions are shown to be important medium constituents.</description><subject>Adipose Tissue - enzymology</subject><subject>Amino Acids - metabolism</subject><subject>Animals</subject><subject>Bicarbonates - pharmacology</subject><subject>Culture Media</subject><subject>Glucose - metabolism</subject><subject>Heparin - pharmacology</subject><subject>Insulin - pharmacology</subject><subject>Lipid Metabolism</subject><subject>Lipoprotein Lipase - metabolism</subject><subject>Lipoproteins - blood</subject><subject>Rats</subject><subject>Starvation</subject><issn>0264-6021</issn><issn>0006-2936</issn><issn>1470-8728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1966</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU9rGzEQxUVpcB23h36Agk6FHjYZ_Vnt6lIIpk0CgVzSs9BqpVhhd-VKWhfnkM8emRjTnGZgfu_NDA-hrwQuCHB62T2BlCA4fEBLwhuo2oa2H9ESqOCVAEo-ofOUngAIBw4LtKil4FKQJXpZD1ZHPz1WTpscIh78VieL_YR177ehtNmnNNsLfIVH2_t5PMz-bbzZ4Lyx2E7P-9HiIvY7n_c4uKMAuxhGnLKOO9vjqHMqQhNtcT90uNAxfEZnTg_JfjnWFfrz-9fD-qa6u7--XV_dVYYzkSvSN8z2hjrHa-Fqyl1Nmk4SISgDSnsBnWlF3VHCiGu5IY456RoDjrSMy5at0M833-3clS-MnXLUg9pGP-q4V0F79X4y-Y16DDtFqKihZsXg-9Eghr-zTVmNPhk7DHqyYU6q5VQ2RIoC_ngDTQwpRetOSwioQ1jqFFZhv_1_1Yk8psNeAQiDkZg</recordid><startdate>19660601</startdate><enddate>19660601</enddate><creator>Salaman, M R</creator><creator>Robinson, D S</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19660601</creationdate><title>Clearing-factor lipase in adipose tissue. 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A medium in which the enzyme activity of tissue from starved rats increases in vitro</atitle><jtitle>Biochemical Journal</jtitle><addtitle>Biochem J</addtitle><date>1966-06-01</date><risdate>1966</risdate><volume>99</volume><issue>3</issue><spage>640</spage><epage>647</epage><pages>640-647</pages><issn>0264-6021</issn><issn>0006-2936</issn><eissn>1470-8728</eissn><abstract>1. When epididymal fat bodies from starved rats are incubated for 3.5hr. at 37 degrees in a defined medium in vitro the total clearing-factor lipase activity rises to approximately twice its initial value. 2. During the incubation period part of the tissue clearing-factor lipase activity appears in the medium. 3. Heparin, glucose, insulin, and HCO(3) (-) and K(+) ions are shown to be important medium constituents.</abstract><cop>England</cop><pmid>5964961</pmid><doi>10.1042/bj0990640</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
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subjects	Adipose Tissue - enzymology Amino Acids - metabolism Animals Bicarbonates - pharmacology Culture Media Glucose - metabolism Heparin - pharmacology Insulin - pharmacology Lipid Metabolism Lipoprotein Lipase - metabolism Lipoproteins - blood Rats Starvation
title	Clearing-factor lipase in adipose tissue. A medium in which the enzyme activity of tissue from starved rats increases in vitro
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