Drosophila ERCC1 is required for a subset of MEI-9-dependent meiotic crossovers
Drosophila MEI-9 is the catalytic subunit of a DNA structure-specific endonuclease required for nucleotide excision repair (NER). The enzymatic activity of this endonuclease during NER requires the presence of a second, noncatalytic subunit called ERCC1. In addition to its role in NER, MEI-9 is requ...
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| Veröffentlicht in: | Genetics (Austin) 2005-08, Vol.170 (4), p.1737-1745 |
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| creator | Radford, Sarah J Goley, Elizabeth Baxter, Kimberly McMahan, Susan Sekelsky, Jeff |
| description | Drosophila MEI-9 is the catalytic subunit of a DNA structure-specific endonuclease required for nucleotide excision repair (NER). The enzymatic activity of this endonuclease during NER requires the presence of a second, noncatalytic subunit called ERCC1. In addition to its role in NER, MEI-9 is required for the generation of most meiotic crossovers. To better understand the role of MEI-9 in crossover formation, we report here the characterization of the Drosophila Ercc1 gene. We created an Ercc1 mutant through homologous gene targeting. We find that Ercc1 mutants are identical to mei-9 mutants in sensitivity to DNA-damaging agents, but have a less severe reduction in the number of meiotic crossovers. MEI-9 protein levels are reduced in Ercc1 mutants; however, overexpression of MEI-9 is not sufficient to restore meiotic crossing over in Ercc1 mutants. We conclude that MEI-9 can generate some meiotic crossovers in an ERCC1-independent manner. |
| doi_str_mv | 10.1534/genetics.104.036178 |
| format | Article |
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The enzymatic activity of this endonuclease during NER requires the presence of a second, noncatalytic subunit called ERCC1. In addition to its role in NER, MEI-9 is required for the generation of most meiotic crossovers. To better understand the role of MEI-9 in crossover formation, we report here the characterization of the Drosophila Ercc1 gene. We created an Ercc1 mutant through homologous gene targeting. We find that Ercc1 mutants are identical to mei-9 mutants in sensitivity to DNA-damaging agents, but have a less severe reduction in the number of meiotic crossovers. MEI-9 protein levels are reduced in Ercc1 mutants; however, overexpression of MEI-9 is not sufficient to restore meiotic crossing over in Ercc1 mutants. We conclude that MEI-9 can generate some meiotic crossovers in an ERCC1-independent manner.</description><identifier>ISSN: 0016-6731</identifier><identifier>ISSN: 1943-2631</identifier><identifier>EISSN: 1943-2631</identifier><identifier>DOI: 10.1534/genetics.104.036178</identifier><identifier>PMID: 15944364</identifier><identifier>CODEN: GENTAE</identifier><language>eng</language><publisher>United States: Genetics Society of America</publisher><subject>Alleles ; Animals ; Crossing Over, Genetic ; Deoxyribonucleic acid ; DNA ; DNA Damage ; DNA repair ; Drosophila ; Drosophila - genetics ; Drosophila Proteins - genetics ; Drosophila Proteins - metabolism ; Endonucleases - chemistry ; Gene Targeting ; Genes, Insect ; Genetic recombination ; Genotype & phenotype ; Insect Proteins - genetics ; Investigations ; Meiosis ; Models, Genetic ; Mutation ; Proteins ; Radiation Tolerance ; Recombinant Fusion Proteins - metabolism ; Two-Hybrid System Techniques ; Ultraviolet Rays</subject><ispartof>Genetics (Austin), 2005-08, Vol.170 (4), p.1737-1745</ispartof><rights>Copyright Genetics Society of America Aug 2005</rights><rights>Copyright © 2005, Genetics Society of America 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c558t-cb56b531bb782f9a67e20ce39e165424e8b1c6e9df4b30a3d60d256d4240f3d43</citedby><cites>FETCH-LOGICAL-c558t-cb56b531bb782f9a67e20ce39e165424e8b1c6e9df4b30a3d60d256d4240f3d43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15944364$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Radford, Sarah J</creatorcontrib><creatorcontrib>Goley, Elizabeth</creatorcontrib><creatorcontrib>Baxter, Kimberly</creatorcontrib><creatorcontrib>McMahan, Susan</creatorcontrib><creatorcontrib>Sekelsky, Jeff</creatorcontrib><title>Drosophila ERCC1 is required for a subset of MEI-9-dependent meiotic crossovers</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>Drosophila MEI-9 is the catalytic subunit of a DNA structure-specific endonuclease required for nucleotide excision repair (NER). The enzymatic activity of this endonuclease during NER requires the presence of a second, noncatalytic subunit called ERCC1. In addition to its role in NER, MEI-9 is required for the generation of most meiotic crossovers. To better understand the role of MEI-9 in crossover formation, we report here the characterization of the Drosophila Ercc1 gene. We created an Ercc1 mutant through homologous gene targeting. We find that Ercc1 mutants are identical to mei-9 mutants in sensitivity to DNA-damaging agents, but have a less severe reduction in the number of meiotic crossovers. MEI-9 protein levels are reduced in Ercc1 mutants; however, overexpression of MEI-9 is not sufficient to restore meiotic crossing over in Ercc1 mutants. We conclude that MEI-9 can generate some meiotic crossovers in an ERCC1-independent manner.</description><subject>Alleles</subject><subject>Animals</subject><subject>Crossing Over, Genetic</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Damage</subject><subject>DNA repair</subject><subject>Drosophila</subject><subject>Drosophila - genetics</subject><subject>Drosophila Proteins - genetics</subject><subject>Drosophila Proteins - metabolism</subject><subject>Endonucleases - chemistry</subject><subject>Gene Targeting</subject><subject>Genes, Insect</subject><subject>Genetic recombination</subject><subject>Genotype & phenotype</subject><subject>Insect Proteins - genetics</subject><subject>Investigations</subject><subject>Meiosis</subject><subject>Models, Genetic</subject><subject>Mutation</subject><subject>Proteins</subject><subject>Radiation Tolerance</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Two-Hybrid System Techniques</subject><subject>Ultraviolet Rays</subject><issn>0016-6731</issn><issn>1943-2631</issn><issn>1943-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkU9v1DAQxS0EokvhEyAhiwO3LB6P7cQXJLQsUKmoEoKz5SST1lU23tpJpX57XHb5e-lppJnfPM28x9hLEGvQqN5e0kRz6PIahFoLNFA3j9gKrMJKGoTHbCUEmMrUCCfsWc7XQghjdfOUnYC2SqFRK3bxIcUc91dh9Hz7dbMBHjJPdLOERD0fYuKe56XNNPM48C_bs8pWPe1p6mma-Y5CLCfwrojkeEspP2dPBj9menGsp-z7x-23zefq_OLT2eb9edVp3cxV12rTaoS2rRs5WG9qkqIjtARGK6moaaEzZPtBtSg89kb0Upu-jMSAvcJT9u6gu1_aHfVduSb50e1T2Pl056IP7t_JFK7cZbx1ILW2cC_w5iiQ4s1CeXa7kDsaRz9RXLIzjda1tPWDIFhdG5T6YbBWEpUSBXz9H3gdlzQVu5wEBYANygLhAfrpbaLh928g3H3-7lf-paHcIf-y9epvW_7sHAPHH5pZrTY</recordid><startdate>200508</startdate><enddate>200508</enddate><creator>Radford, Sarah J</creator><creator>Goley, Elizabeth</creator><creator>Baxter, Kimberly</creator><creator>McMahan, Susan</creator><creator>Sekelsky, Jeff</creator><general>Genetics Society of America</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7QP</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200508</creationdate><title>Drosophila ERCC1 is required for a subset of MEI-9-dependent meiotic crossovers</title><author>Radford, Sarah J ; Goley, Elizabeth ; Baxter, Kimberly ; McMahan, Susan ; Sekelsky, Jeff</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c558t-cb56b531bb782f9a67e20ce39e165424e8b1c6e9df4b30a3d60d256d4240f3d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Alleles</topic><topic>Animals</topic><topic>Crossing Over, Genetic</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Damage</topic><topic>DNA repair</topic><topic>Drosophila</topic><topic>Drosophila - genetics</topic><topic>Drosophila Proteins - genetics</topic><topic>Drosophila Proteins - metabolism</topic><topic>Endonucleases - chemistry</topic><topic>Gene Targeting</topic><topic>Genes, Insect</topic><topic>Genetic recombination</topic><topic>Genotype & phenotype</topic><topic>Insect Proteins - genetics</topic><topic>Investigations</topic><topic>Meiosis</topic><topic>Models, Genetic</topic><topic>Mutation</topic><topic>Proteins</topic><topic>Radiation Tolerance</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Two-Hybrid System Techniques</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Radford, Sarah J</creatorcontrib><creatorcontrib>Goley, Elizabeth</creatorcontrib><creatorcontrib>Baxter, Kimberly</creatorcontrib><creatorcontrib>McMahan, Susan</creatorcontrib><creatorcontrib>Sekelsky, Jeff</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genetics (Austin)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Radford, Sarah J</au><au>Goley, Elizabeth</au><au>Baxter, Kimberly</au><au>McMahan, Susan</au><au>Sekelsky, Jeff</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Drosophila ERCC1 is required for a subset of MEI-9-dependent meiotic crossovers</atitle><jtitle>Genetics (Austin)</jtitle><addtitle>Genetics</addtitle><date>2005-08</date><risdate>2005</risdate><volume>170</volume><issue>4</issue><spage>1737</spage><epage>1745</epage><pages>1737-1745</pages><issn>0016-6731</issn><issn>1943-2631</issn><eissn>1943-2631</eissn><coden>GENTAE</coden><abstract>Drosophila MEI-9 is the catalytic subunit of a DNA structure-specific endonuclease required for nucleotide excision repair (NER). The enzymatic activity of this endonuclease during NER requires the presence of a second, noncatalytic subunit called ERCC1. In addition to its role in NER, MEI-9 is required for the generation of most meiotic crossovers. To better understand the role of MEI-9 in crossover formation, we report here the characterization of the Drosophila Ercc1 gene. We created an Ercc1 mutant through homologous gene targeting. We find that Ercc1 mutants are identical to mei-9 mutants in sensitivity to DNA-damaging agents, but have a less severe reduction in the number of meiotic crossovers. MEI-9 protein levels are reduced in Ercc1 mutants; however, overexpression of MEI-9 is not sufficient to restore meiotic crossing over in Ercc1 mutants. We conclude that MEI-9 can generate some meiotic crossovers in an ERCC1-independent manner.</abstract><cop>United States</cop><pub>Genetics Society of America</pub><pmid>15944364</pmid><doi>10.1534/genetics.104.036178</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Alleles Animals Crossing Over, Genetic Deoxyribonucleic acid DNA DNA Damage DNA repair Drosophila Drosophila - genetics Drosophila Proteins - genetics Drosophila Proteins - metabolism Endonucleases - chemistry Gene Targeting Genes, Insect Genetic recombination Genotype & phenotype Insect Proteins - genetics Investigations Meiosis Models, Genetic Mutation Proteins Radiation Tolerance Recombinant Fusion Proteins - metabolism Two-Hybrid System Techniques Ultraviolet Rays |
| title | Drosophila ERCC1 is required for a subset of MEI-9-dependent meiotic crossovers |
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