Control of Specific Gene Expression by Gibberellin and Brassinosteroid1
We identified a recessive, brassinolide-insensitive mutant caused by a deletion allele ( bri1-201 ) of the brassinosteroid (BR) receptor BRI1 . The bri1-201 mutant displayed altered expression levels of genes differentially regulated by gibberellin (GA). RNA-blot analysis revealed that BR and GA ant...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2001-10, Vol.127 (2), p.450-458 |
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| container_title | Plant physiology (Bethesda) |
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| creator | Bouquin, Thomas Meier, Carsten Foster, Randy Nielsen, Mads Eggert Mundy, John |
| description | We identified a recessive, brassinolide-insensitive mutant caused by a deletion allele (
bri1-201
) of the brassinosteroid (BR) receptor
BRI1
. The
bri1-201
mutant displayed altered expression levels of genes differentially regulated by gibberellin (GA). RNA-blot analysis revealed that BR and GA antagonistically regulate the accumulation of mRNAs of the GA-responsive
GASA1
gene, as well as the GA-repressible
GA5
gene. Expression studies with cycloheximide indicated that the antagonistic effects of GA and BR on
GA5
require de novo protein synthesis. Reporter transgene analyses and RNA-blot analysis showed that BR and GA modulate
GA5
expression, at least in part, at the transcriptional level, and that the signals are independent and subtractive. |
| format | Article |
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bri1-201
) of the brassinosteroid (BR) receptor
BRI1
. The
bri1-201
mutant displayed altered expression levels of genes differentially regulated by gibberellin (GA). RNA-blot analysis revealed that BR and GA antagonistically regulate the accumulation of mRNAs of the GA-responsive
GASA1
gene, as well as the GA-repressible
GA5
gene. Expression studies with cycloheximide indicated that the antagonistic effects of GA and BR on
GA5
require de novo protein synthesis. Reporter transgene analyses and RNA-blot analysis showed that BR and GA modulate
GA5
expression, at least in part, at the transcriptional level, and that the signals are independent and subtractive.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>PMID: 11598220</identifier><language>eng</language><publisher>American Society of Plant Physiologists</publisher><ispartof>Plant physiology (Bethesda), 2001-10, Vol.127 (2), p.450-458</ispartof><rights>Copyright © 2001, American Society of Plant Physiologists 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,782,786,887</link.rule.ids></links><search><creatorcontrib>Bouquin, Thomas</creatorcontrib><creatorcontrib>Meier, Carsten</creatorcontrib><creatorcontrib>Foster, Randy</creatorcontrib><creatorcontrib>Nielsen, Mads Eggert</creatorcontrib><creatorcontrib>Mundy, John</creatorcontrib><title>Control of Specific Gene Expression by Gibberellin and Brassinosteroid1</title><title>Plant physiology (Bethesda)</title><description>We identified a recessive, brassinolide-insensitive mutant caused by a deletion allele (
bri1-201
) of the brassinosteroid (BR) receptor
BRI1
. The
bri1-201
mutant displayed altered expression levels of genes differentially regulated by gibberellin (GA). RNA-blot analysis revealed that BR and GA antagonistically regulate the accumulation of mRNAs of the GA-responsive
GASA1
gene, as well as the GA-repressible
GA5
gene. Expression studies with cycloheximide indicated that the antagonistic effects of GA and BR on
GA5
require de novo protein synthesis. Reporter transgene analyses and RNA-blot analysis showed that BR and GA modulate
GA5
expression, at least in part, at the transcriptional level, and that the signals are independent and subtractive.</description><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNqljb0KwjAURoMotv68Q15ASNIW0sHFUuuue0jaW70Sk5Ko6NvbwcXZ6Xxw-DgTkvIiExtR5HJKUsbGzaQsE7KI8coY4xnP5yThvCilECwlTeXdPXhLfU-PA7TYY0sbcEDr1xAgRvSOmjdt0BgIYC06ql1Hd0GPzvl4h-Cx4ysy67WNsP5ySbb7-lQdNsPD3KBrYaxoq4aANx3eymtUv8bhRZ39U3FRMMmzf_8fLbdVJA</recordid><startdate>20011001</startdate><enddate>20011001</enddate><creator>Bouquin, Thomas</creator><creator>Meier, Carsten</creator><creator>Foster, Randy</creator><creator>Nielsen, Mads Eggert</creator><creator>Mundy, John</creator><general>American Society of Plant Physiologists</general><scope>5PM</scope></search><sort><creationdate>20011001</creationdate><title>Control of Specific Gene Expression by Gibberellin and Brassinosteroid1</title><author>Bouquin, Thomas ; Meier, Carsten ; Foster, Randy ; Nielsen, Mads Eggert ; Mundy, John</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmedcentral_primary_oai_pubmedcentral_nih_gov_1250813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bouquin, Thomas</creatorcontrib><creatorcontrib>Meier, Carsten</creatorcontrib><creatorcontrib>Foster, Randy</creatorcontrib><creatorcontrib>Nielsen, Mads Eggert</creatorcontrib><creatorcontrib>Mundy, John</creatorcontrib><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bouquin, Thomas</au><au>Meier, Carsten</au><au>Foster, Randy</au><au>Nielsen, Mads Eggert</au><au>Mundy, John</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Control of Specific Gene Expression by Gibberellin and Brassinosteroid1</atitle><jtitle>Plant physiology (Bethesda)</jtitle><date>2001-10-01</date><risdate>2001</risdate><volume>127</volume><issue>2</issue><spage>450</spage><epage>458</epage><pages>450-458</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><abstract>We identified a recessive, brassinolide-insensitive mutant caused by a deletion allele (
bri1-201
) of the brassinosteroid (BR) receptor
BRI1
. The
bri1-201
mutant displayed altered expression levels of genes differentially regulated by gibberellin (GA). RNA-blot analysis revealed that BR and GA antagonistically regulate the accumulation of mRNAs of the GA-responsive
GASA1
gene, as well as the GA-repressible
GA5
gene. Expression studies with cycloheximide indicated that the antagonistic effects of GA and BR on
GA5
require de novo protein synthesis. Reporter transgene analyses and RNA-blot analysis showed that BR and GA modulate
GA5
expression, at least in part, at the transcriptional level, and that the signals are independent and subtractive.</abstract><pub>American Society of Plant Physiologists</pub><pmid>11598220</pmid></addata></record> |
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| ispartof | Plant physiology (Bethesda), 2001-10, Vol.127 (2), p.450-458 |
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| language | eng |
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| source | JSTOR Archive Collection A-Z Listing; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| title | Control of Specific Gene Expression by Gibberellin and Brassinosteroid1 |
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