Electrotonic coupling in internally perfused crayfish segmented axons
We have developed a technique for cannulation and internal perfusion of crayfish segmented lateral axons. Experiments on perfused and non-perfused axons lead to the following conclusions: 1. Internally perfused segmented axons behave very similarly to non-perfused axons. 2. The axial electrical resi...
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| Veröffentlicht in: | The Journal of physiology 1981-08, Vol.317 (1), p.509-518 |
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| container_title | The Journal of physiology |
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| creator | Johnston, M.F Ramon, F |
| description | We have developed a technique for cannulation and internal perfusion of crayfish segmented lateral axons. Experiments on perfused
and non-perfused axons lead to the following conclusions: 1. Internally perfused segmented axons behave very similarly to
non-perfused axons. 2. The axial electrical resistance of the junctional region is almost as low as a comparable segment of
axon. 3. Neither intracellular Ca2+ nor H+ is effective in disrupting the intercellular communication pathway in perfused
axons. On the basis of these findings we have formulated a hypothesis for cellular control of intercellular coupling based
on the existence of a soluble intermediate for Ca2+ or H+-induced uncoupling. This hypothesis is consistent with data from
both internally perfused and non-perfused axons. |
| doi_str_mv | 10.1113/jphysiol.1981.sp013840 |
| format | Article |
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and non-perfused axons lead to the following conclusions: 1. Internally perfused segmented axons behave very similarly to
non-perfused axons. 2. The axial electrical resistance of the junctional region is almost as low as a comparable segment of
axon. 3. Neither intracellular Ca2+ nor H+ is effective in disrupting the intercellular communication pathway in perfused
axons. On the basis of these findings we have formulated a hypothesis for cellular control of intercellular coupling based
on the existence of a soluble intermediate for Ca2+ or H+-induced uncoupling. This hypothesis is consistent with data from
both internally perfused and non-perfused axons.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.1981.sp013840</identifier><identifier>PMID: 6796679</identifier><language>eng</language><publisher>England: The Physiological Society</publisher><subject>Animals ; Astacoidea - physiology ; Axons - drug effects ; Axons - physiology ; Calcium - pharmacology ; Cambaridae ; Electric Conductivity ; Glutaral - pharmacology ; Hydrogen-Ion Concentration ; In Vitro Techniques ; Membrane Potentials ; Octanols - pharmacology ; Procambarus clarkii</subject><ispartof>The Journal of physiology, 1981-08, Vol.317 (1), p.509-518</ispartof><rights>1981 The Physiological Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5579-6eb1d8409f1227aa32f38535435ff0fb8cf4606702728083e88522fb05ca5e333</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1246804/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1246804/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,1418,27929,27930,45579,45580,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6796679$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Johnston, M.F</creatorcontrib><creatorcontrib>Ramon, F</creatorcontrib><title>Electrotonic coupling in internally perfused crayfish segmented axons</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>We have developed a technique for cannulation and internal perfusion of crayfish segmented lateral axons. Experiments on perfused
and non-perfused axons lead to the following conclusions: 1. Internally perfused segmented axons behave very similarly to
non-perfused axons. 2. The axial electrical resistance of the junctional region is almost as low as a comparable segment of
axon. 3. Neither intracellular Ca2+ nor H+ is effective in disrupting the intercellular communication pathway in perfused
axons. On the basis of these findings we have formulated a hypothesis for cellular control of intercellular coupling based
on the existence of a soluble intermediate for Ca2+ or H+-induced uncoupling. This hypothesis is consistent with data from
both internally perfused and non-perfused axons.</description><subject>Animals</subject><subject>Astacoidea - physiology</subject><subject>Axons - drug effects</subject><subject>Axons - physiology</subject><subject>Calcium - pharmacology</subject><subject>Cambaridae</subject><subject>Electric Conductivity</subject><subject>Glutaral - pharmacology</subject><subject>Hydrogen-Ion Concentration</subject><subject>In Vitro Techniques</subject><subject>Membrane Potentials</subject><subject>Octanols - pharmacology</subject><subject>Procambarus clarkii</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkktv1DAUhS0EKkPhJwBZAZsMfsSvDRKthpcqgUS7tjweO-PKE6d2Qpt_j6NMK9ggJFuWfM_97rGOAXiF4BohRN5f9_sp-xjWSAq0zj1ERDTwEVihhsmac0kegxWEGNeEU_QUPMv5GhYRlPIEnDAuWdkrsNkEa4YUh9h5U5k49sF3beW7sgabOh3CVPU2uTHbXWWSnpzP-yrb9mCLYFfpu9jl5-CJ0yHbF8fzFFx92lyef6kvvn_-ev7xojaUclkzu0W74lI6hDHXmmBHBCW0IdQ56LbCuIZBxiHmWEBBrBAUY7eF1GhqCSGn4MPC7cftwe5MsZB0UH3yB50mFbVXf1c6v1dt_KUQbpiATQG8OQJSvBltHtTBZ2ND0J2NY1ac8GIAzZPe_VOIOJRIMMhnJlukJsWck3UPfhBUc1bqPis1Z6XusyqNL_98zUPbMZxSP1vqtz7Y6T-p6vLbj_mCII4onCFvF8jet_tbn6xa2nI03g6TKjqF1KJ8vSidjkq3yWd19RPPXwZJ1kAsyG_AMr-7</recordid><startdate>19810801</startdate><enddate>19810801</enddate><creator>Johnston, M.F</creator><creator>Ramon, F</creator><general>The Physiological Society</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19810801</creationdate><title>Electrotonic coupling in internally perfused crayfish segmented axons</title><author>Johnston, M.F ; Ramon, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5579-6eb1d8409f1227aa32f38535435ff0fb8cf4606702728083e88522fb05ca5e333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Animals</topic><topic>Astacoidea - physiology</topic><topic>Axons - drug effects</topic><topic>Axons - physiology</topic><topic>Calcium - pharmacology</topic><topic>Cambaridae</topic><topic>Electric Conductivity</topic><topic>Glutaral - pharmacology</topic><topic>Hydrogen-Ion Concentration</topic><topic>In Vitro Techniques</topic><topic>Membrane Potentials</topic><topic>Octanols - pharmacology</topic><topic>Procambarus clarkii</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Johnston, M.F</creatorcontrib><creatorcontrib>Ramon, F</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Johnston, M.F</au><au>Ramon, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrotonic coupling in internally perfused crayfish segmented axons</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1981-08-01</date><risdate>1981</risdate><volume>317</volume><issue>1</issue><spage>509</spage><epage>518</epage><pages>509-518</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><abstract>We have developed a technique for cannulation and internal perfusion of crayfish segmented lateral axons. Experiments on perfused
and non-perfused axons lead to the following conclusions: 1. Internally perfused segmented axons behave very similarly to
non-perfused axons. 2. The axial electrical resistance of the junctional region is almost as low as a comparable segment of
axon. 3. Neither intracellular Ca2+ nor H+ is effective in disrupting the intercellular communication pathway in perfused
axons. On the basis of these findings we have formulated a hypothesis for cellular control of intercellular coupling based
on the existence of a soluble intermediate for Ca2+ or H+-induced uncoupling. This hypothesis is consistent with data from
both internally perfused and non-perfused axons.</abstract><cop>England</cop><pub>The Physiological Society</pub><pmid>6796679</pmid><doi>10.1113/jphysiol.1981.sp013840</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of physiology, 1981-08, Vol.317 (1), p.509-518 |
| issn | 0022-3751 1469-7793 |
| language | eng |
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| source | MEDLINE; Access via Wiley Online Library; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Astacoidea - physiology Axons - drug effects Axons - physiology Calcium - pharmacology Cambaridae Electric Conductivity Glutaral - pharmacology Hydrogen-Ion Concentration In Vitro Techniques Membrane Potentials Octanols - pharmacology Procambarus clarkii |
| title | Electrotonic coupling in internally perfused crayfish segmented axons |
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