An in vivo dual-luciferase assay system for studying translational recoding in the yeast Saccharomyces cerevisiae

A new in vivo assay system has been developed to study programmed frameshifting in the yeast Saccharomyces cerevisiae. Frameshift signals are inserted between the Renilla and firefly luciferase reporter genes contained in a yeast expression vector and the two activities are directly measured from ce...

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Veröffentlicht in:	RNA (Cambridge) 2003-08, Vol.9 (8), p.1019-1024
Hauptverfasser:	Harger, Jason W, Dinman, Jonathan D
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Coleoptera Frameshifting, Ribosomal Genes, Reporter Luciferases - metabolism Method Molecular Sequence Data Protein Biosynthesis RNA, Messenger - genetics Saccharomyces cerevisiae - genetics
Online-Zugang:	Volltext
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