Comparison of serological techniques to measure antibody to Pasteurella haemolytica A1
Analysis of 45 sera was performed employing five techniques which are currently in use in three laboratories to measure anti-Pasteurella haemolytica antibodies. The enzyme linked immunosorbent assay, passive hemagglutination, complement fixation and direct and indirect bacterial agglutination assays...
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| Veröffentlicht in: | Canadian journal of comparative medicine 1985, Vol.49 (1), p.99-103 |
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| container_title | Canadian journal of comparative medicine |
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| creator | FILION, L. G CHO, H. J SHEWEN, P. E RAYBOULD, T. J. G WILKIE, B. N |
| description | Analysis of 45 sera was performed employing five techniques which are currently in use in three laboratories to measure anti-Pasteurella haemolytica antibodies. The enzyme linked immunosorbent assay, passive hemagglutination, complement fixation and direct and indirect bacterial agglutination assays were employed and a relationship between tests in the measurement of anti-P. haemolytica antibodies was demonstrated. Regression analysis together with prediction and confidence intervals were tabulated also. The conclusion drawn from statistical analysis was that all five tests are similar in their ability to detect immune responses (antibody and antigen(s) interactions) to Pasteurella haemolytica. |
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The conclusion drawn from statistical analysis was that all five tests are similar in their ability to detect immune responses (antibody and antigen(s) interactions) to Pasteurella haemolytica.</description><identifier>ISSN: 0008-4050</identifier><identifier>PMID: 3986685</identifier><language>eng</language><publisher>Ottawa, ON: Canadian Veterinary Medical Association</publisher><subject>Animals ; Antibodies, Bacterial - analysis ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biological and medical sciences ; Cattle ; Complement Fixation Tests ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. 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The conclusion drawn from statistical analysis was that all five tests are similar in their ability to detect immune responses (antibody and antigen(s) interactions) to Pasteurella haemolytica.</description><subject>Animals</subject><subject>Antibodies, Bacterial - analysis</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Complement Fixation Tests</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemagglutination Tests</subject><subject>Microbiology</subject><subject>Pasteurella - immunology</subject><subject>Regression Analysis</subject><subject>Serology - methods</subject><issn>0008-4050</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUMFKxDAU7EFZ19VPEHIQb4WkTdPmIiyLusKCHtRreU1fdyNtU5NU2L83xbLo6cHMvBlmzqIlpbSIOc3oRXTp3CelPBVSLKJFKgshimwZfWxMN4DVzvTENMShNa3ZawUt8agOvf4a0RFvSIfgRosEeq8rUx8n7BWcxwC2LZADYGfaow-vZM2uovMGWofX811F748Pb5ttvHt5et6sd_GQSOpjbBgTjLNEiZxXtcqYApWLgksh6ooqyTjkGaa1FFhXokDVyDxUaCpJK85Zuoruf32HseqwVth7C205WN2BPZYGdPmf6fWh3JvvkiWpYIkIBnezgTVTVV922qmpUY9mdGUu6DRUEoQ3f5NOEfOSgb-deXBhvcZCr7Q7yYosoyzIfgAC_n22</recordid><startdate>1985</startdate><enddate>1985</enddate><creator>FILION, L. G</creator><creator>CHO, H. J</creator><creator>SHEWEN, P. E</creator><creator>RAYBOULD, T. J. G</creator><creator>WILKIE, B. 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E</creatorcontrib><creatorcontrib>RAYBOULD, T. J. G</creatorcontrib><creatorcontrib>WILKIE, B. N</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Canadian journal of comparative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FILION, L. G</au><au>CHO, H. J</au><au>SHEWEN, P. E</au><au>RAYBOULD, T. J. G</au><au>WILKIE, B. N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of serological techniques to measure antibody to Pasteurella haemolytica A1</atitle><jtitle>Canadian journal of comparative medicine</jtitle><addtitle>Can J Comp Med</addtitle><date>1985</date><risdate>1985</risdate><volume>49</volume><issue>1</issue><spage>99</spage><epage>103</epage><pages>99-103</pages><issn>0008-4050</issn><abstract>Analysis of 45 sera was performed employing five techniques which are currently in use in three laboratories to measure anti-Pasteurella haemolytica antibodies. The enzyme linked immunosorbent assay, passive hemagglutination, complement fixation and direct and indirect bacterial agglutination assays were employed and a relationship between tests in the measurement of anti-P. haemolytica antibodies was demonstrated. Regression analysis together with prediction and confidence intervals were tabulated also. The conclusion drawn from statistical analysis was that all five tests are similar in their ability to detect immune responses (antibody and antigen(s) interactions) to Pasteurella haemolytica.</abstract><cop>Ottawa, ON</cop><pub>Canadian Veterinary Medical Association</pub><pmid>3986685</pmid><tpages>5</tpages></addata></record> |
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| ispartof | Canadian journal of comparative medicine, 1985, Vol.49 (1), p.99-103 |
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| language | eng |
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| subjects | Animals Antibodies, Bacterial - analysis Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Cattle Complement Fixation Tests Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Hemagglutination Tests Microbiology Pasteurella - immunology Regression Analysis Serology - methods |
| title | Comparison of serological techniques to measure antibody to Pasteurella haemolytica A1 |
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