Catalysis of S-Nitrosothiols Formation by Serum Albumin: The Mechanism and Implication in Vascular Control
Nitric oxide (NO·) is a short-lived physiological messenger. Its various biological activities can be preserved in a more stable form of S-nitrosothiols (RS-NO). Here we demonstrate that at physiological NO· concentrations, plasma albumin becomes saturated with NO· and accelerates formation of low-m...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2002-04, Vol.99 (9), p.5913-5918 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Rafikova, Olga Rafikov, Ruslan Nudler, Evgeny |
| description | Nitric oxide (NO·) is a short-lived physiological messenger. Its various biological activities can be preserved in a more stable form of S-nitrosothiols (RS-NO). Here we demonstrate that at physiological NO· concentrations, plasma albumin becomes saturated with NO· and accelerates formation of low-molecular-weight (LMW) RS-NO in vitro and in vivo. The mechanism involves micellar catalysis of NO· oxidation in the albumin hydrophobic core and specific transfer of NO+ to LMW thiols. Albumin-mediated S-nitrosylation and its vasodilatory effect directly depend on the concentration of circulating LMW thiols. Results suggest that the hydrophobic phase formed by albumin serves as a major reservoir of NO· and its reactive oxides and controls the dynamics of NO·-dependant processes in the vasculature. |
| doi_str_mv | 10.1073/pnas.092048999 |
| format | Article |
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Its various biological activities can be preserved in a more stable form of S-nitrosothiols (RS-NO). Here we demonstrate that at physiological NO· concentrations, plasma albumin becomes saturated with NO· and accelerates formation of low-molecular-weight (LMW) RS-NO in vitro and in vivo. The mechanism involves micellar catalysis of NO· oxidation in the albumin hydrophobic core and specific transfer of NO+ to LMW thiols. Albumin-mediated S-nitrosylation and its vasodilatory effect directly depend on the concentration of circulating LMW thiols. Results suggest that the hydrophobic phase formed by albumin serves as a major reservoir of NO· and its reactive oxides and controls the dynamics of NO·-dependant processes in the vasculature.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.092048999</identifier><identifier>PMID: 11983891</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Albumins ; Albumins - metabolism ; Animals ; Biochemistry ; Biological Sciences ; Blood ; Blood plasma ; Blood pressure ; Blood vessels ; Catalysis ; Dose-Response Relationship, Drug ; Experimental procedures ; Femoral Artery - metabolism ; Femoral Vein - metabolism ; Male ; Micelles ; Models, Biological ; Molecules ; Nitric Oxide - chemistry ; Nitric Oxide - metabolism ; Nitrites ; Nitrites - metabolism ; Oxidation ; Proteins ; Rats ; Rats, Wistar ; S-Nitrosothiols - metabolism ; Sulfhydryl Compounds - metabolism ; Thiols ; Time Factors ; Water - chemistry</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2002-04, Vol.99 (9), p.5913-5918</ispartof><rights>Copyright 1993-2002 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Apr 30, 2002</rights><rights>Copyright © 2002, The National Academy of Sciences 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c486t-8fa22acecadfcbaf64e153ebf129ab954a1b0387352b5278035c471138fc3aac3</citedby><cites>FETCH-LOGICAL-c486t-8fa22acecadfcbaf64e153ebf129ab954a1b0387352b5278035c471138fc3aac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/99/9.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3058599$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3058599$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11983891$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rafikova, Olga</creatorcontrib><creatorcontrib>Rafikov, Ruslan</creatorcontrib><creatorcontrib>Nudler, Evgeny</creatorcontrib><title>Catalysis of S-Nitrosothiols Formation by Serum Albumin: The Mechanism and Implication in Vascular Control</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Nitric oxide (NO·) is a short-lived physiological messenger. Its various biological activities can be preserved in a more stable form of S-nitrosothiols (RS-NO). Here we demonstrate that at physiological NO· concentrations, plasma albumin becomes saturated with NO· and accelerates formation of low-molecular-weight (LMW) RS-NO in vitro and in vivo. The mechanism involves micellar catalysis of NO· oxidation in the albumin hydrophobic core and specific transfer of NO+ to LMW thiols. Albumin-mediated S-nitrosylation and its vasodilatory effect directly depend on the concentration of circulating LMW thiols. Results suggest that the hydrophobic phase formed by albumin serves as a major reservoir of NO· and its reactive oxides and controls the dynamics of NO·-dependant processes in the vasculature.</description><subject>Albumins</subject><subject>Albumins - metabolism</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biological Sciences</subject><subject>Blood</subject><subject>Blood plasma</subject><subject>Blood pressure</subject><subject>Blood vessels</subject><subject>Catalysis</subject><subject>Dose-Response Relationship, Drug</subject><subject>Experimental procedures</subject><subject>Femoral Artery - metabolism</subject><subject>Femoral Vein - metabolism</subject><subject>Male</subject><subject>Micelles</subject><subject>Models, Biological</subject><subject>Molecules</subject><subject>Nitric Oxide - chemistry</subject><subject>Nitric Oxide - metabolism</subject><subject>Nitrites</subject><subject>Nitrites - metabolism</subject><subject>Oxidation</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>S-Nitrosothiols - metabolism</subject><subject>Sulfhydryl Compounds - metabolism</subject><subject>Thiols</subject><subject>Time Factors</subject><subject>Water - chemistry</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1vEzEYhC0EomnhygmQxYHbBn_u2kgcqqillQocWrha7zpe4si7DvYuIv8eR0lDe-HkwzwzHr2D0CtK5pQ0_MNmgDwnmhGhtNZP0IwSTataaPIUzQhhTaUEEyfoNOc1IURLRZ6jE0q14krTGVovYISwzT7j2OHb6qsfU8xxXPkYMr6MqYfRxwG3W3zr0tTj89BOvR8-4ruVw1-cXcHgc49hWOLrfhO83fN-wD8g2ylAwos4lNDwAj3rIGT38vCeoe-XF3eLq-rm2-frxflNZYWqx0p1wBhYZ2HZ2Ra6WjgquWs7yjS0WgqgLeGq4ZK1kjWKcGlFQylXneUAlp-hT_vczdT2bmld-R2C2STfQ9qaCN48Vga_Mj_jb0MZU01d_O8O_hR_TS6PZh2nNJTKhhHKtagbVaD5HrLlXDm57phPidktY3bLmOMyxfD2Yat_-GGKArw5ADvjvay10UYWuejv_6ebbgphdH_GAr7eg-s8xnQkOZFKliJ_AbwzrWc</recordid><startdate>20020430</startdate><enddate>20020430</enddate><creator>Rafikova, Olga</creator><creator>Rafikov, Ruslan</creator><creator>Nudler, Evgeny</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><general>The National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20020430</creationdate><title>Catalysis of S-Nitrosothiols Formation by Serum Albumin: The Mechanism and Implication in Vascular Control</title><author>Rafikova, Olga ; Rafikov, Ruslan ; Nudler, Evgeny</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c486t-8fa22acecadfcbaf64e153ebf129ab954a1b0387352b5278035c471138fc3aac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Albumins</topic><topic>Albumins - metabolism</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Biological Sciences</topic><topic>Blood</topic><topic>Blood plasma</topic><topic>Blood pressure</topic><topic>Blood vessels</topic><topic>Catalysis</topic><topic>Dose-Response Relationship, Drug</topic><topic>Experimental procedures</topic><topic>Femoral Artery - metabolism</topic><topic>Femoral Vein - metabolism</topic><topic>Male</topic><topic>Micelles</topic><topic>Models, Biological</topic><topic>Molecules</topic><topic>Nitric Oxide - chemistry</topic><topic>Nitric Oxide - metabolism</topic><topic>Nitrites</topic><topic>Nitrites - metabolism</topic><topic>Oxidation</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>S-Nitrosothiols - metabolism</topic><topic>Sulfhydryl Compounds - metabolism</topic><topic>Thiols</topic><topic>Time Factors</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rafikova, Olga</creatorcontrib><creatorcontrib>Rafikov, Ruslan</creatorcontrib><creatorcontrib>Nudler, Evgeny</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rafikova, Olga</au><au>Rafikov, Ruslan</au><au>Nudler, Evgeny</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Catalysis of S-Nitrosothiols Formation by Serum Albumin: The Mechanism and Implication in Vascular Control</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2002-04-30</date><risdate>2002</risdate><volume>99</volume><issue>9</issue><spage>5913</spage><epage>5918</epage><pages>5913-5918</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Nitric oxide (NO·) is a short-lived physiological messenger. Its various biological activities can be preserved in a more stable form of S-nitrosothiols (RS-NO). Here we demonstrate that at physiological NO· concentrations, plasma albumin becomes saturated with NO· and accelerates formation of low-molecular-weight (LMW) RS-NO in vitro and in vivo. The mechanism involves micellar catalysis of NO· oxidation in the albumin hydrophobic core and specific transfer of NO+ to LMW thiols. Albumin-mediated S-nitrosylation and its vasodilatory effect directly depend on the concentration of circulating LMW thiols. Results suggest that the hydrophobic phase formed by albumin serves as a major reservoir of NO· and its reactive oxides and controls the dynamics of NO·-dependant processes in the vasculature.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>11983891</pmid><doi>10.1073/pnas.092048999</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Albumins Albumins - metabolism Animals Biochemistry Biological Sciences Blood Blood plasma Blood pressure Blood vessels Catalysis Dose-Response Relationship, Drug Experimental procedures Femoral Artery - metabolism Femoral Vein - metabolism Male Micelles Models, Biological Molecules Nitric Oxide - chemistry Nitric Oxide - metabolism Nitrites Nitrites - metabolism Oxidation Proteins Rats Rats, Wistar S-Nitrosothiols - metabolism Sulfhydryl Compounds - metabolism Thiols Time Factors Water - chemistry |
| title | Catalysis of S-Nitrosothiols Formation by Serum Albumin: The Mechanism and Implication in Vascular Control |
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