The relationship of agarose gel structure to the sieving of spheres during agarose gel electrophoresis

To understand the organization of fibers in an agarose gel, digitized electron micrographs are used here to determine the frequency distribution of interfiber distance (2Pc) in thin sections of agarose gels. For a preparation of underivatized agarose, a 1.5% gel has a Pc distribution that is indisti...

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Veröffentlicht in:	Biophysical journal 1993-07, Vol.65 (1), p.138-148
Hauptverfasser:	Griess, G.A., Guiseley, K.B., Serwer, P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biophysical Phenomena Biophysics DNA - chemistry DNA - isolation & purification Electrophoresis, Agar Gel Fundamental and applied biological sciences. Psychology Gels - chemistry General aspects Instrumentation. Materials. Reagents. Research laboratory organization Microscopy, Electron Particle Size Polymers - chemistry Polymers - isolation & purification Sepharose - chemistry
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