Mammalian NADH diphosphatases of the Nudix family: cloning and characterization of the human peroxisomal NUDT12 protein

The human NUDT12 Nudix hydrolase has been expressed in insect cells from a baculovirus vector as a His-tagged recombinant protein. In vitro, it efficiently hydrolyses NAD(P)H to NMNH and AMP (2',5'-ADP), and diadenosine diphosphate to AMP. It also has activity towards NAD(P)(+), ADP-ribose...

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Veröffentlicht in:	Biochemical journal 2003-09, Vol.374 (Pt 2), p.329-335
Hauptverfasser:	Abdelraheim, Salama R, Spiller, David G, McLennan, Alexander G
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amygdala - enzymology Animals Baculoviridae - enzymology Baculoviridae - genetics Cell Line Cloning, Molecular - methods DNA, Complementary - isolation & purification Genetic Vectors Green Fluorescent Proteins Humans Luminescent Proteins - biosynthesis Luminescent Proteins - genetics Molecular Sequence Data Multigene Family Peroxisomes - chemistry Peroxisomes - enzymology Pyrophosphatases - biosynthesis Pyrophosphatases - chemistry Pyrophosphatases - genetics Pyrophosphatases - isolation & purification Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Sequence Alignment Spodoptera Substrate Specificity
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