Limnanthes douglasii lysophosphatidic acid acyltransferases: immunological quantification, acyl selectivity and functional replacement of the Escherichia coli plsC gene
Antibodies were raised against the two membrane-bound lysophosphatidic acid acyltransferase (LPAAT) enzymes from Limnanthes douglasii (meadowfoam), LAT1 and LAT2, using the predicted soluble portion of each protein as recombinant protein antigens. The antibodies can distinguish between the two acylt...
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| Veröffentlicht in: | Biochemical journal 2002-06, Vol.364 (Pt 3), p.795-805 |
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| creator | Brown, Adrian P Carnaby, Simon Brough, Clare Brazier, Melissa Slabas, Antoni R |
| description | Antibodies were raised against the two membrane-bound lysophosphatidic acid acyltransferase (LPAAT) enzymes from Limnanthes douglasii (meadowfoam), LAT1 and LAT2, using the predicted soluble portion of each protein as recombinant protein antigens. The antibodies can distinguish between the two acyltransferase proteins and demonstrate that both migrate in an anomalous fashion on SDS/PAGE gels. The antibodies were used to determine that LAT1 is present in both leaf and developing seeds, whereas LAT2 is only detectable in developing seeds later than 22 daf (days after flowering). Both proteins were found exclusively in microsomal fractions and their amount was determined using the recombinant antigens as quantification standards. LAT1 is present at a level of 27 pg/microg of membrane protein in leaf tissue and |
| doi_str_mv | 10.1042/BJ20020071 |
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The antibodies can distinguish between the two acyltransferase proteins and demonstrate that both migrate in an anomalous fashion on SDS/PAGE gels. The antibodies were used to determine that LAT1 is present in both leaf and developing seeds, whereas LAT2 is only detectable in developing seeds later than 22 daf (days after flowering). Both proteins were found exclusively in microsomal fractions and their amount was determined using the recombinant antigens as quantification standards. LAT1 is present at a level of 27 pg/microg of membrane protein in leaf tissue and <or=12.5 pg/microg of membrane protein in developing embryos. The amount of LAT2 reaches a peak at 305 pg/microg of membrane protein 25 daf and is not expressed 20 daf or before. This is the first study to quantify these membrane-bound proteins in a plant tissue. The maximal level of LAT2 protein coincides with the maximal level of erucic acid synthesis in the seeds. Both full-length proteins were expressed in the Escherichia coli LPAAT mutant JC201, and membranes from these strains were used to investigate the substrate selectivity of these two enzymes, demonstrating that they are different. Finally, we report that LAT2 and a maize LPAAT enzyme (MAT1) can functionally replace the E. coli plsC gene after its deletion in the chromosome, whereas LAT1 and a coconut LPAAT (Coco1) cannot. This is probably due to differences in substrate utilization.</description><identifier>ISSN: 0264-6021</identifier><identifier>EISSN: 1470-8728</identifier><identifier>DOI: 10.1042/BJ20020071</identifier><identifier>PMID: 12049644</identifier><language>eng</language><publisher>England</publisher><subject>1-Acylglycerol-3-Phosphate O-Acyltransferase ; Acyltransferases - analysis ; Acyltransferases - genetics ; Acyltransferases - metabolism ; Amino Acid Sequence ; Antibodies ; Antibody Specificity ; Blotting, Western ; Cell Membrane - enzymology ; Cloning, Molecular ; Escherichia coli - enzymology ; Escherichia coli - genetics ; Escherichia coli Proteins ; Immunoassay ; Isoenzymes - analysis ; Isoenzymes - genetics ; Isoenzymes - metabolism ; Kinetics ; Molecular Sequence Data ; Plant Stems - enzymology ; Plants - enzymology ; Recombinant Proteins - metabolism</subject><ispartof>Biochemical journal, 2002-06, Vol.364 (Pt 3), p.795-805</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-d011a7f93b976f92bfea184218714dbe47946b7e70593ee2e88bd5cf57e94de53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1222629/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1222629/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12049644$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brown, Adrian P</creatorcontrib><creatorcontrib>Carnaby, Simon</creatorcontrib><creatorcontrib>Brough, Clare</creatorcontrib><creatorcontrib>Brazier, Melissa</creatorcontrib><creatorcontrib>Slabas, Antoni R</creatorcontrib><title>Limnanthes douglasii lysophosphatidic acid acyltransferases: immunological quantification, acyl selectivity and functional replacement of the Escherichia coli plsC gene</title><title>Biochemical journal</title><addtitle>Biochem J</addtitle><description>Antibodies were raised against the two membrane-bound lysophosphatidic acid acyltransferase (LPAAT) enzymes from Limnanthes douglasii (meadowfoam), LAT1 and LAT2, using the predicted soluble portion of each protein as recombinant protein antigens. The antibodies can distinguish between the two acyltransferase proteins and demonstrate that both migrate in an anomalous fashion on SDS/PAGE gels. The antibodies were used to determine that LAT1 is present in both leaf and developing seeds, whereas LAT2 is only detectable in developing seeds later than 22 daf (days after flowering). Both proteins were found exclusively in microsomal fractions and their amount was determined using the recombinant antigens as quantification standards. LAT1 is present at a level of 27 pg/microg of membrane protein in leaf tissue and <or=12.5 pg/microg of membrane protein in developing embryos. The amount of LAT2 reaches a peak at 305 pg/microg of membrane protein 25 daf and is not expressed 20 daf or before. This is the first study to quantify these membrane-bound proteins in a plant tissue. The maximal level of LAT2 protein coincides with the maximal level of erucic acid synthesis in the seeds. Both full-length proteins were expressed in the Escherichia coli LPAAT mutant JC201, and membranes from these strains were used to investigate the substrate selectivity of these two enzymes, demonstrating that they are different. Finally, we report that LAT2 and a maize LPAAT enzyme (MAT1) can functionally replace the E. coli plsC gene after its deletion in the chromosome, whereas LAT1 and a coconut LPAAT (Coco1) cannot. This is probably due to differences in substrate utilization.</description><subject>1-Acylglycerol-3-Phosphate O-Acyltransferase</subject><subject>Acyltransferases - analysis</subject><subject>Acyltransferases - genetics</subject><subject>Acyltransferases - metabolism</subject><subject>Amino Acid Sequence</subject><subject>Antibodies</subject><subject>Antibody Specificity</subject><subject>Blotting, Western</subject><subject>Cell Membrane - enzymology</subject><subject>Cloning, Molecular</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli Proteins</subject><subject>Immunoassay</subject><subject>Isoenzymes - analysis</subject><subject>Isoenzymes - genetics</subject><subject>Isoenzymes - metabolism</subject><subject>Kinetics</subject><subject>Molecular Sequence Data</subject><subject>Plant Stems - enzymology</subject><subject>Plants - enzymology</subject><subject>Recombinant Proteins - metabolism</subject><issn>0264-6021</issn><issn>1470-8728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk2LFDEQhoMo7rh68QdITh7E1iST7nR7WNBh_WLAi56bdLoyXUs66U3SC_OP_Jlm3fHrJBSpQD28PFBFyFPOXnEmxet3nwVjpRS_RzZcKla1SrT3yYaJRlYNE_yMPErpijEumWQPyRkXTHaNlBvyfY-z1z5PkOgY1oPTCZG6YwrLFNIy6YwjGqoNjuU5uhy1TxaiTpDeUJzn1QcXDmi0o9drCUJb_hmDf_mTpwkcmIw3mI9U-5Ha1ZvbceEjLE4bmMFnGiwtDvQymQkimgk1NcEhXVza0QN4eEweWO0SPDn1c_Lt_eXX3cdq_-XDp93bfWWkbHI1Ms61st126FRjOzFY0LyVgreKy3EAqTrZDAoUq7stgIC2Hcba2FpBJ0eot-fk4i53WYcZRlPkonb9EnHW8dgHjf2_E49Tfwg3PRdCNKIrAc9PATFcr5ByP2My4Jz2ENbUK65a3rH_g7wtirVqC_jiDjQxpBTB_rbhrL-9gH64-nUBBX72t_8f9LTy7Q_SobHZ</recordid><startdate>20020615</startdate><enddate>20020615</enddate><creator>Brown, Adrian P</creator><creator>Carnaby, Simon</creator><creator>Brough, Clare</creator><creator>Brazier, Melissa</creator><creator>Slabas, Antoni R</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20020615</creationdate><title>Limnanthes douglasii lysophosphatidic acid acyltransferases: immunological quantification, acyl selectivity and functional replacement of the Escherichia coli plsC gene</title><author>Brown, Adrian P ; Carnaby, Simon ; Brough, Clare ; Brazier, Melissa ; Slabas, Antoni R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-d011a7f93b976f92bfea184218714dbe47946b7e70593ee2e88bd5cf57e94de53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>1-Acylglycerol-3-Phosphate O-Acyltransferase</topic><topic>Acyltransferases - analysis</topic><topic>Acyltransferases - genetics</topic><topic>Acyltransferases - metabolism</topic><topic>Amino Acid Sequence</topic><topic>Antibodies</topic><topic>Antibody Specificity</topic><topic>Blotting, Western</topic><topic>Cell Membrane - enzymology</topic><topic>Cloning, Molecular</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli Proteins</topic><topic>Immunoassay</topic><topic>Isoenzymes - analysis</topic><topic>Isoenzymes - genetics</topic><topic>Isoenzymes - metabolism</topic><topic>Kinetics</topic><topic>Molecular Sequence Data</topic><topic>Plant Stems - enzymology</topic><topic>Plants - enzymology</topic><topic>Recombinant Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brown, Adrian P</creatorcontrib><creatorcontrib>Carnaby, Simon</creatorcontrib><creatorcontrib>Brough, Clare</creatorcontrib><creatorcontrib>Brazier, Melissa</creatorcontrib><creatorcontrib>Slabas, Antoni R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biochemical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brown, Adrian P</au><au>Carnaby, Simon</au><au>Brough, Clare</au><au>Brazier, Melissa</au><au>Slabas, Antoni R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Limnanthes douglasii lysophosphatidic acid acyltransferases: immunological quantification, acyl selectivity and functional replacement of the Escherichia coli plsC gene</atitle><jtitle>Biochemical journal</jtitle><addtitle>Biochem J</addtitle><date>2002-06-15</date><risdate>2002</risdate><volume>364</volume><issue>Pt 3</issue><spage>795</spage><epage>805</epage><pages>795-805</pages><issn>0264-6021</issn><eissn>1470-8728</eissn><abstract>Antibodies were raised against the two membrane-bound lysophosphatidic acid acyltransferase (LPAAT) enzymes from Limnanthes douglasii (meadowfoam), LAT1 and LAT2, using the predicted soluble portion of each protein as recombinant protein antigens. The antibodies can distinguish between the two acyltransferase proteins and demonstrate that both migrate in an anomalous fashion on SDS/PAGE gels. The antibodies were used to determine that LAT1 is present in both leaf and developing seeds, whereas LAT2 is only detectable in developing seeds later than 22 daf (days after flowering). Both proteins were found exclusively in microsomal fractions and their amount was determined using the recombinant antigens as quantification standards. LAT1 is present at a level of 27 pg/microg of membrane protein in leaf tissue and <or=12.5 pg/microg of membrane protein in developing embryos. The amount of LAT2 reaches a peak at 305 pg/microg of membrane protein 25 daf and is not expressed 20 daf or before. This is the first study to quantify these membrane-bound proteins in a plant tissue. The maximal level of LAT2 protein coincides with the maximal level of erucic acid synthesis in the seeds. Both full-length proteins were expressed in the Escherichia coli LPAAT mutant JC201, and membranes from these strains were used to investigate the substrate selectivity of these two enzymes, demonstrating that they are different. Finally, we report that LAT2 and a maize LPAAT enzyme (MAT1) can functionally replace the E. coli plsC gene after its deletion in the chromosome, whereas LAT1 and a coconut LPAAT (Coco1) cannot. This is probably due to differences in substrate utilization.</abstract><cop>England</cop><pmid>12049644</pmid><doi>10.1042/BJ20020071</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 1-Acylglycerol-3-Phosphate O-Acyltransferase Acyltransferases - analysis Acyltransferases - genetics Acyltransferases - metabolism Amino Acid Sequence Antibodies Antibody Specificity Blotting, Western Cell Membrane - enzymology Cloning, Molecular Escherichia coli - enzymology Escherichia coli - genetics Escherichia coli Proteins Immunoassay Isoenzymes - analysis Isoenzymes - genetics Isoenzymes - metabolism Kinetics Molecular Sequence Data Plant Stems - enzymology Plants - enzymology Recombinant Proteins - metabolism |
| title | Limnanthes douglasii lysophosphatidic acid acyltransferases: immunological quantification, acyl selectivity and functional replacement of the Escherichia coli plsC gene |
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