Novel method for evaluation of the oligomeric structure of membrane proteins

Assessment of the quaternary structure of membrane proteins by PAGE has been problematic owing to their relatively poor solubility in non-dissociative detergents. Here we report that several membrane proteins can be readily solubilized in their native quaternary structure with the use of the deterge...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemical journal 1999-08, Vol.342 (1), p.119-123
Hauptverfasser:	Ramjeesingh, M, Garami, E, Bear, C.E
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Anion Exchange Protein 1, Erythrocyte - chemistry Anion Exchange Protein 1, Erythrocyte - isolation & purification Aquaporin 1 Aquaporins - chemistry Aquaporins - isolation & purification Blood Group Antigens Blotting, Western Caprylates Cell Membrane - chemistry chemical structure Cytoplasm - chemistry Detergents Electrophoresis, Polyacrylamide Gel Fluorocarbons Humans ion channels membrane proteins Membrane Proteins - chemistry Membrane Proteins - isolation & purification molecular conformation Molecular Weight Potassium Channels - chemistry Potassium Channels - isolation & purification Potassium Channels, Inwardly Rectifying Protein Conformation protein structure protein subunits Rats Receptors, GABA-A - chemistry Receptors, GABA-A - isolation & purification Solubility Xenopus laevis
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!