Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor-cellulose-binding domain fusion protein
A chimaera of stem cell factor (SCF) and a cellulose-binding domain from the xylanase Cex (CBDCex) effectively immobilizes SCF on a cellulose surface. The fusion protein retains both the cytokine properties of SCF and the cellulose-binding characteristics of CBDCex. When adsorbed on cellulose, SCF-C...
Gespeichert in:
| Veröffentlicht in: | Biochemical journal 1999-04, Vol.339 (2), p.429-434 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 434 |
|---|---|
| container_issue | 2 |
| container_start_page | 429 |
| container_title | Biochemical journal |
| container_volume | 339 |
| creator | Doheny, J.G Jervis, E.J Guarna, M.M Humphries, R.K Warren, R.A.J Kilburn, D.G |
| description | A chimaera of stem cell factor (SCF) and a cellulose-binding domain from the xylanase Cex (CBDCex) effectively immobilizes SCF on a cellulose surface. The fusion protein retains both the cytokine properties of SCF and the cellulose-binding characteristics of CBDCex. When adsorbed on cellulose, SCF-CBDCex is up to 7-fold more potent than soluble SCF-CBDCex and than native SCF at stimulating the proliferation of factor-dependent cell lines. When cells are incubated with cellulose-bound SCF-CBDCex, activated receptors and SCF-CBDCex co-localize on the cellulose matrix. The strong binding of SCF-CBDCex to the cellulose surface permits the effective and localized stimulation of target cells; this is potentially significant for long-term perfusion culturing of factor-dependent cells. It also permits the direct analysis of the effects of surface-bound cytokines on target cells. |
| doi_str_mv | 10.1042/0264-6021:3390429 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1220174</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69674037</sourcerecordid><originalsourceid>FETCH-LOGICAL-c485t-858296d844952f420c85d8f6df98c84a1e40991d6cb2faf4b5de7b451050b4613</originalsourceid><addsrcrecordid>eNpVkctu1DAUhi0EotPCA7ABr9gFjh0nsbtAQiNuUqUuSteW48tgSOzBdir6Mn1WHGao2pVln-9851g_Qq8IvCPA6HugPWt6oOS8bUV9EE_QhrABGj5Q_hRt7usn6DTnnwCEAYPn6IQAEYQO_Qbdbe00LVPMFquMVcA-2FTwrEryf7CLCe-TzTYUH3ZY35b4qwIZl4iLSjtbsK79-bxS0Sy6-BiqxKzXffX4ikaHFc7Fzv9Q7JQuMTX6_9hm9MGschNn5QN2S14lVVCsDy_QM6embF8ezzN0_fnT9-3X5uLyy7ftx4tGM96Vhnecit5wxkRHHaOgeWe4640TXHOmiGUgBDG9HqlTjo2dscPIOgIdjKwn7Rn6cPDul3G2RtcPJzXJffKzSrcyKi8fV4L_IXfxRhJKgQysCt4eBSn-XmwucvZ5_aQKNi5Z9qIfGLRDBckB1CnmnKy7H0JArqnKNTW5piaPqdae1w-3e9BxiLECbw6AU1GqXfJZXl_VxVqgAnjL2vYvnBmq3Q</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69674037</pqid></control><display><type>article</type><title>Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor-cellulose-binding domain fusion protein</title><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>EZB Electronic Journals Library</source><creator>Doheny, J.G ; Jervis, E.J ; Guarna, M.M ; Humphries, R.K ; Warren, R.A.J ; Kilburn, D.G</creator><creatorcontrib>Doheny, J.G ; Jervis, E.J ; Guarna, M.M ; Humphries, R.K ; Warren, R.A.J ; Kilburn, D.G</creatorcontrib><description>A chimaera of stem cell factor (SCF) and a cellulose-binding domain from the xylanase Cex (CBDCex) effectively immobilizes SCF on a cellulose surface. The fusion protein retains both the cytokine properties of SCF and the cellulose-binding characteristics of CBDCex. When adsorbed on cellulose, SCF-CBDCex is up to 7-fold more potent than soluble SCF-CBDCex and than native SCF at stimulating the proliferation of factor-dependent cell lines. When cells are incubated with cellulose-bound SCF-CBDCex, activated receptors and SCF-CBDCex co-localize on the cellulose matrix. The strong binding of SCF-CBDCex to the cellulose surface permits the effective and localized stimulation of target cells; this is potentially significant for long-term perfusion culturing of factor-dependent cells. It also permits the direct analysis of the effects of surface-bound cytokines on target cells.</description><identifier>ISSN: 0264-6021</identifier><identifier>EISSN: 1470-8728</identifier><identifier>DOI: 10.1042/0264-6021:3390429</identifier><identifier>PMID: 10191276</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; binding ; Cell Division ; Cell Line ; cellulose ; Cellulose - metabolism ; Cytokines - biosynthesis ; Fluorescent Antibody Technique ; growth factors ; Humans ; Mice ; Microscopy, Confocal ; Protein Binding ; Recombinant Fusion Proteins - metabolism ; Stem Cell Factor - biosynthesis ; Stem Cell Factor - metabolism</subject><ispartof>Biochemical journal, 1999-04, Vol.339 (2), p.429-434</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c485t-858296d844952f420c85d8f6df98c84a1e40991d6cb2faf4b5de7b451050b4613</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1220174/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1220174/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10191276$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Doheny, J.G</creatorcontrib><creatorcontrib>Jervis, E.J</creatorcontrib><creatorcontrib>Guarna, M.M</creatorcontrib><creatorcontrib>Humphries, R.K</creatorcontrib><creatorcontrib>Warren, R.A.J</creatorcontrib><creatorcontrib>Kilburn, D.G</creatorcontrib><title>Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor-cellulose-binding domain fusion protein</title><title>Biochemical journal</title><addtitle>Biochem J</addtitle><description>A chimaera of stem cell factor (SCF) and a cellulose-binding domain from the xylanase Cex (CBDCex) effectively immobilizes SCF on a cellulose surface. The fusion protein retains both the cytokine properties of SCF and the cellulose-binding characteristics of CBDCex. When adsorbed on cellulose, SCF-CBDCex is up to 7-fold more potent than soluble SCF-CBDCex and than native SCF at stimulating the proliferation of factor-dependent cell lines. When cells are incubated with cellulose-bound SCF-CBDCex, activated receptors and SCF-CBDCex co-localize on the cellulose matrix. The strong binding of SCF-CBDCex to the cellulose surface permits the effective and localized stimulation of target cells; this is potentially significant for long-term perfusion culturing of factor-dependent cells. It also permits the direct analysis of the effects of surface-bound cytokines on target cells.</description><subject>Animals</subject><subject>binding</subject><subject>Cell Division</subject><subject>Cell Line</subject><subject>cellulose</subject><subject>Cellulose - metabolism</subject><subject>Cytokines - biosynthesis</subject><subject>Fluorescent Antibody Technique</subject><subject>growth factors</subject><subject>Humans</subject><subject>Mice</subject><subject>Microscopy, Confocal</subject><subject>Protein Binding</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Stem Cell Factor - biosynthesis</subject><subject>Stem Cell Factor - metabolism</subject><issn>0264-6021</issn><issn>1470-8728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkctu1DAUhi0EotPCA7ABr9gFjh0nsbtAQiNuUqUuSteW48tgSOzBdir6Mn1WHGao2pVln-9851g_Qq8IvCPA6HugPWt6oOS8bUV9EE_QhrABGj5Q_hRt7usn6DTnnwCEAYPn6IQAEYQO_Qbdbe00LVPMFquMVcA-2FTwrEryf7CLCe-TzTYUH3ZY35b4qwIZl4iLSjtbsK79-bxS0Sy6-BiqxKzXffX4ikaHFc7Fzv9Q7JQuMTX6_9hm9MGschNn5QN2S14lVVCsDy_QM6embF8ezzN0_fnT9-3X5uLyy7ftx4tGM96Vhnecit5wxkRHHaOgeWe4640TXHOmiGUgBDG9HqlTjo2dscPIOgIdjKwn7Rn6cPDul3G2RtcPJzXJffKzSrcyKi8fV4L_IXfxRhJKgQysCt4eBSn-XmwucvZ5_aQKNi5Z9qIfGLRDBckB1CnmnKy7H0JArqnKNTW5piaPqdae1w-3e9BxiLECbw6AU1GqXfJZXl_VxVqgAnjL2vYvnBmq3Q</recordid><startdate>19990415</startdate><enddate>19990415</enddate><creator>Doheny, J.G</creator><creator>Jervis, E.J</creator><creator>Guarna, M.M</creator><creator>Humphries, R.K</creator><creator>Warren, R.A.J</creator><creator>Kilburn, D.G</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19990415</creationdate><title>Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor-cellulose-binding domain fusion protein</title><author>Doheny, J.G ; Jervis, E.J ; Guarna, M.M ; Humphries, R.K ; Warren, R.A.J ; Kilburn, D.G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-858296d844952f420c85d8f6df98c84a1e40991d6cb2faf4b5de7b451050b4613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>binding</topic><topic>Cell Division</topic><topic>Cell Line</topic><topic>cellulose</topic><topic>Cellulose - metabolism</topic><topic>Cytokines - biosynthesis</topic><topic>Fluorescent Antibody Technique</topic><topic>growth factors</topic><topic>Humans</topic><topic>Mice</topic><topic>Microscopy, Confocal</topic><topic>Protein Binding</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Stem Cell Factor - biosynthesis</topic><topic>Stem Cell Factor - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Doheny, J.G</creatorcontrib><creatorcontrib>Jervis, E.J</creatorcontrib><creatorcontrib>Guarna, M.M</creatorcontrib><creatorcontrib>Humphries, R.K</creatorcontrib><creatorcontrib>Warren, R.A.J</creatorcontrib><creatorcontrib>Kilburn, D.G</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biochemical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Doheny, J.G</au><au>Jervis, E.J</au><au>Guarna, M.M</au><au>Humphries, R.K</au><au>Warren, R.A.J</au><au>Kilburn, D.G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor-cellulose-binding domain fusion protein</atitle><jtitle>Biochemical journal</jtitle><addtitle>Biochem J</addtitle><date>1999-04-15</date><risdate>1999</risdate><volume>339</volume><issue>2</issue><spage>429</spage><epage>434</epage><pages>429-434</pages><issn>0264-6021</issn><eissn>1470-8728</eissn><abstract>A chimaera of stem cell factor (SCF) and a cellulose-binding domain from the xylanase Cex (CBDCex) effectively immobilizes SCF on a cellulose surface. The fusion protein retains both the cytokine properties of SCF and the cellulose-binding characteristics of CBDCex. When adsorbed on cellulose, SCF-CBDCex is up to 7-fold more potent than soluble SCF-CBDCex and than native SCF at stimulating the proliferation of factor-dependent cell lines. When cells are incubated with cellulose-bound SCF-CBDCex, activated receptors and SCF-CBDCex co-localize on the cellulose matrix. The strong binding of SCF-CBDCex to the cellulose surface permits the effective and localized stimulation of target cells; this is potentially significant for long-term perfusion culturing of factor-dependent cells. It also permits the direct analysis of the effects of surface-bound cytokines on target cells.</abstract><cop>England</cop><pmid>10191276</pmid><doi>10.1042/0264-6021:3390429</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-6021 |
| ispartof | Biochemical journal, 1999-04, Vol.339 (2), p.429-434 |
| issn | 0264-6021 1470-8728 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1220174 |
| source | MEDLINE; PubMed Central; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Animals binding Cell Division Cell Line cellulose Cellulose - metabolism Cytokines - biosynthesis Fluorescent Antibody Technique growth factors Humans Mice Microscopy, Confocal Protein Binding Recombinant Fusion Proteins - metabolism Stem Cell Factor - biosynthesis Stem Cell Factor - metabolism |
| title | Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor-cellulose-binding domain fusion protein |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T18%3A10%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cellulose%20as%20an%20inert%20matrix%20for%20presenting%20cytokines%20to%20target%20cells:%20production%20and%20properties%20of%20a%20stem%20cell%20factor-cellulose-binding%20domain%20fusion%20protein&rft.jtitle=Biochemical%20journal&rft.au=Doheny,%20J.G&rft.date=1999-04-15&rft.volume=339&rft.issue=2&rft.spage=429&rft.epage=434&rft.pages=429-434&rft.issn=0264-6021&rft.eissn=1470-8728&rft_id=info:doi/10.1042/0264-6021:3390429&rft_dat=%3Cproquest_pubme%3E69674037%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69674037&rft_id=info:pmid/10191276&rfr_iscdi=true |