Albumin binding of unconjugated [3H]bilirubin and its uptake by rat liver basolateral plasma membrane vesicles

Using highly purified unconjugated [3H]bilirubin (UCB), we measured UCB binding to delipidated human serum albumin (HSA) and its uptake by basolateral rat liver plasma membrane vesicles, in both the absence and presence of an inside-positive membrane potential. Free UCB concentrations ([Bf]) were ca...

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Veröffentlicht in:	Biochemical journal 1996-06, Vol.316 ( Pt 3) (3), p.999-1004
Hauptverfasser:	Pascolo, L, Del Vecchio, S, Koehler, R K, Bayon, J E, Webster, C C, Mukerjee, P, Ostrow, J D, Tiribelli, C
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bilirubin - metabolism Biological Transport - drug effects Cell Membrane - drug effects Cell Membrane - metabolism Cells, Cultured Female Humans Kinetics Liver - drug effects Liver - metabolism Osmolar Concentration Potassium Chloride - pharmacology Protein Binding Rats Rats, Wistar Regression Analysis Serum Albumin - metabolism Sulfobromophthalein - pharmacology
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