The role of residues glutamate-50 and phenylalanine-496 in Zymomonas mobilis pyruvate decarboxylase

Several enzymes require thiamine diphosphate (ThDP) as an essential cofactor, and we have used one of these, pyruvate decarboxylase (PDC; EC 4.1.1.1) from Zymomonas mobilis, as a model for this group of enzymes. It is well suited for this purpose because of its stability, ease of purification, homot...

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Veröffentlicht in:	Biochemical journal 1996-05, Vol.315 ( Pt 3) (3), p.745-751
Hauptverfasser:	Candy, J M, Koga, J, Nixon, P F, Duggleby, R G
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Binding Sites - genetics Escherichia coli - genetics Glutamic Acid - chemistry Kinetics Models, Molecular Molecular Sequence Data Molecular Structure Mutagenesis, Site-Directed Phenylalanine - chemistry Protein Conformation Pyruvate Decarboxylase - chemistry Pyruvate Decarboxylase - genetics Pyruvate Decarboxylase - metabolism Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Sequence Homology, Amino Acid Thiamine Pyrophosphate - metabolism Zymomonas - enzymology Zymomonas - genetics Zymomonas mobilis
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