Pentamidine uptake in Leishmania donovani and Leishmania amazonensis promastigotes and axenic amastigotes
A transport system for pentamidine in Leishmania donovani and Leishmania amazonensis promastigotes and axenic amastigotes has been identified and characterized. Pentamidine is not metabolized by these parasites. Its uptake process is saturable, carrier-mediated and energy-dependent. This drug does n...
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| Veröffentlicht in: | Biochemical journal 1996-04, Vol.315 (2), p.631-634 |
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| container_title | Biochemical journal |
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| creator | Basselin, M Lawrence, F Robert-Gero, M |
| description | A transport system for pentamidine in Leishmania donovani and Leishmania amazonensis promastigotes and axenic amastigotes has been identified and characterized. Pentamidine is not metabolized by these parasites. Its uptake process is saturable, carrier-mediated and energy-dependent. This drug does not inhibit purine or pyrimidine uptake, whereas it inhibits uptake of several amino acids non-competitively and that of putrescine and spermidine competitively. The results suggest that pentamidine shares polyamine-carrier systems in these parasites. |
| doi_str_mv | 10.1042/bj3150631 |
| format | Article |
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The results suggest that pentamidine shares polyamine-carrier systems in these parasites.</description><identifier>ISSN: 0264-6021</identifier><identifier>EISSN: 1470-8728</identifier><identifier>DOI: 10.1042/bj3150631</identifier><identifier>PMID: 8615840</identifier><language>eng</language><publisher>England</publisher><subject>amastigotes ; Animals ; Antiprotozoal Agents - metabolism ; Antiprotozoal Agents - pharmacokinetics ; Biological Transport, Active ; inhibition ; Kinetics ; Leishmania amazonensis ; Leishmania donovani ; Leishmania donovani - growth & development ; Leishmania donovani - metabolism ; Leishmania mexicana - growth & development ; Leishmania mexicana - metabolism ; pentamidine ; Pentamidine - metabolism ; Pentamidine - pharmacokinetics ; promastigotes ; putrescine ; Putrescine - pharmacokinetics ; spermidine ; Spermidine - pharmacokinetics ; transport processes ; uptake</subject><ispartof>Biochemical journal, 1996-04, Vol.315 (2), p.631-634</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-78549aef3112a113966103e2457ee1828a6a1ccf02a1a68f7d40294b3d4d12c63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1217243/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1217243/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8615840$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Basselin, M</creatorcontrib><creatorcontrib>Lawrence, F</creatorcontrib><creatorcontrib>Robert-Gero, M</creatorcontrib><title>Pentamidine uptake in Leishmania donovani and Leishmania amazonensis promastigotes and axenic amastigotes</title><title>Biochemical journal</title><addtitle>Biochem J</addtitle><description>A transport system for pentamidine in Leishmania donovani and Leishmania amazonensis promastigotes and axenic amastigotes has been identified and characterized. Pentamidine is not metabolized by these parasites. Its uptake process is saturable, carrier-mediated and energy-dependent. This drug does not inhibit purine or pyrimidine uptake, whereas it inhibits uptake of several amino acids non-competitively and that of putrescine and spermidine competitively. The results suggest that pentamidine shares polyamine-carrier systems in these parasites.</description><subject>amastigotes</subject><subject>Animals</subject><subject>Antiprotozoal Agents - metabolism</subject><subject>Antiprotozoal Agents - pharmacokinetics</subject><subject>Biological Transport, Active</subject><subject>inhibition</subject><subject>Kinetics</subject><subject>Leishmania amazonensis</subject><subject>Leishmania donovani</subject><subject>Leishmania donovani - growth & development</subject><subject>Leishmania donovani - metabolism</subject><subject>Leishmania mexicana - growth & development</subject><subject>Leishmania mexicana - metabolism</subject><subject>pentamidine</subject><subject>Pentamidine - metabolism</subject><subject>Pentamidine - pharmacokinetics</subject><subject>promastigotes</subject><subject>putrescine</subject><subject>Putrescine - pharmacokinetics</subject><subject>spermidine</subject><subject>Spermidine - pharmacokinetics</subject><subject>transport processes</subject><subject>uptake</subject><issn>0264-6021</issn><issn>1470-8728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFP3DAQha0KRBfaQ39A1ZyQOARmbMdxLkgIAUVaqZUoZ2s2mSymG3sbZ1HLryd0lxWcOM1o3qenN3pCfEE4RtDyZHavsACj8IOYoC4ht6W0O2IC0ujcgMSPYj-lewDUoGFP7FmDhdUwEf4nh4E63_jA2Wo50G_OfMim7NNdR8FT1sQQH8Yto9C8vlNHjzFwSD5lyz52lAY_jwOn_yD95eDrZ-jl_EnstrRI_HkzD8Tt5cWv8-_59MfV9fnZNK-1LIa8tIWuiFuFKAlRVcYgKJa6KJnRSkuGsK5bGFUyti0bDbLSM9XoBmVt1IE4XfsuV7OOm3r8r6eFW_a-o_6fi-TdWyX4OzePDw4lllKr0eBwY9DHPytOg-t8qnmxoMBxlVxpAdEAvAtiYaw0JY7g0Rqs-5hSz-02DYJ7LtBtCxzZr6_jb8lNY6P-ba23FB3Ne5_c7Y0EVIAFVlhp9QRi1qD5</recordid><startdate>19960415</startdate><enddate>19960415</enddate><creator>Basselin, M</creator><creator>Lawrence, F</creator><creator>Robert-Gero, M</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19960415</creationdate><title>Pentamidine uptake in Leishmania donovani and Leishmania amazonensis promastigotes and axenic amastigotes</title><author>Basselin, M ; Lawrence, F ; Robert-Gero, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-78549aef3112a113966103e2457ee1828a6a1ccf02a1a68f7d40294b3d4d12c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>amastigotes</topic><topic>Animals</topic><topic>Antiprotozoal Agents - metabolism</topic><topic>Antiprotozoal Agents - pharmacokinetics</topic><topic>Biological Transport, Active</topic><topic>inhibition</topic><topic>Kinetics</topic><topic>Leishmania amazonensis</topic><topic>Leishmania donovani</topic><topic>Leishmania donovani - growth & development</topic><topic>Leishmania donovani - metabolism</topic><topic>Leishmania mexicana - growth & development</topic><topic>Leishmania mexicana - metabolism</topic><topic>pentamidine</topic><topic>Pentamidine - metabolism</topic><topic>Pentamidine - pharmacokinetics</topic><topic>promastigotes</topic><topic>putrescine</topic><topic>Putrescine - pharmacokinetics</topic><topic>spermidine</topic><topic>Spermidine - pharmacokinetics</topic><topic>transport processes</topic><topic>uptake</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Basselin, M</creatorcontrib><creatorcontrib>Lawrence, F</creatorcontrib><creatorcontrib>Robert-Gero, M</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biochemical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Basselin, M</au><au>Lawrence, F</au><au>Robert-Gero, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pentamidine uptake in Leishmania donovani and Leishmania amazonensis promastigotes and axenic amastigotes</atitle><jtitle>Biochemical journal</jtitle><addtitle>Biochem J</addtitle><date>1996-04-15</date><risdate>1996</risdate><volume>315</volume><issue>2</issue><spage>631</spage><epage>634</epage><pages>631-634</pages><issn>0264-6021</issn><eissn>1470-8728</eissn><abstract>A transport system for pentamidine in Leishmania donovani and Leishmania amazonensis promastigotes and axenic amastigotes has been identified and characterized. Pentamidine is not metabolized by these parasites. Its uptake process is saturable, carrier-mediated and energy-dependent. This drug does not inhibit purine or pyrimidine uptake, whereas it inhibits uptake of several amino acids non-competitively and that of putrescine and spermidine competitively. The results suggest that pentamidine shares polyamine-carrier systems in these parasites.</abstract><cop>England</cop><pmid>8615840</pmid><doi>10.1042/bj3150631</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-6021 |
| ispartof | Biochemical journal, 1996-04, Vol.315 (2), p.631-634 |
| issn | 0264-6021 1470-8728 |
| language | eng |
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| source | MEDLINE; PubMed Central; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | amastigotes Animals Antiprotozoal Agents - metabolism Antiprotozoal Agents - pharmacokinetics Biological Transport, Active inhibition Kinetics Leishmania amazonensis Leishmania donovani Leishmania donovani - growth & development Leishmania donovani - metabolism Leishmania mexicana - growth & development Leishmania mexicana - metabolism pentamidine Pentamidine - metabolism Pentamidine - pharmacokinetics promastigotes putrescine Putrescine - pharmacokinetics spermidine Spermidine - pharmacokinetics transport processes uptake |
| title | Pentamidine uptake in Leishmania donovani and Leishmania amazonensis promastigotes and axenic amastigotes |
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