Molecular and genetic analysis of REC103, an early meiotic recombination gene in yeast
In the yeast Saccharomyces cerevisiae at least 10 genes are required to begin meiotic recombination. A new early recombination gene REC103 is described in this paper. It was initially defined by the rec103-1 mutation found in a selection for mutations overcoming the spore inviability of a rad52 spo1...
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| Veröffentlicht in: | Genetics (Austin) 1997-08, Vol.146 (4), p.1265-1274 |
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| creator | Gardiner, J.M. (University of Iowa, Iowa City, IA.) Bullard, S.A Chrome, C Malone, R.E |
| description | In the yeast Saccharomyces cerevisiae at least 10 genes are required to begin meiotic recombination. A new early recombination gene REC103 is described in this paper. It was initially defined by the rec103-1 mutation found in a selection for mutations overcoming the spore inviability of a rad52 spo13 haploid strain. Mutations in REC103 also rescue rad52 in spo13 diploids. rec103 spo13 strains produce viable spores; these spores show no evidence of meiotic recombination. rec103 SPO13 diploids produce no viable spores, consistent with loss of recombination. Mutations in REC103 do not affect mitotic recombination, growth, or repair. These phenotypes are identical to those conferred by mutations in several other early meiotic recombination genes (e.g., REC102, REC104, REC114, ME14, MER2, and SPO11). REC103 maps to chromosome VII between ADE5 and RAD54. Cloning and sequencing of REC103 reveals that REC103 is identical to SK18, a gene that depresses the expression of yeast double-stranded ("killer") (ds)RNA viruses. REC103/SK18 is transcribed in mitotic cells and is induced approximately 15-fold in meiosis. REC103 has 26% amino acid identity to the Schizasaccharomyces pombe rec14+ gene; mutations in both genes confer similar meiotic phenotypes, suggesting that they may play similar roles in meiotic recombination. |
| doi_str_mv | 10.1093/genetics/146.4.1265 |
| format | Article |
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(University of Iowa, Iowa City, IA.) ; Bullard, S.A ; Chrome, C ; Malone, R.E</creator><creatorcontrib>Gardiner, J.M. (University of Iowa, Iowa City, IA.) ; Bullard, S.A ; Chrome, C ; Malone, R.E</creatorcontrib><description>In the yeast Saccharomyces cerevisiae at least 10 genes are required to begin meiotic recombination. A new early recombination gene REC103 is described in this paper. It was initially defined by the rec103-1 mutation found in a selection for mutations overcoming the spore inviability of a rad52 spo13 haploid strain. Mutations in REC103 also rescue rad52 in spo13 diploids. rec103 spo13 strains produce viable spores; these spores show no evidence of meiotic recombination. rec103 SPO13 diploids produce no viable spores, consistent with loss of recombination. Mutations in REC103 do not affect mitotic recombination, growth, or repair. These phenotypes are identical to those conferred by mutations in several other early meiotic recombination genes (e.g., REC102, REC104, REC114, ME14, MER2, and SPO11). REC103 maps to chromosome VII between ADE5 and RAD54. Cloning and sequencing of REC103 reveals that REC103 is identical to SK18, a gene that depresses the expression of yeast double-stranded ("killer") (ds)RNA viruses. REC103/SK18 is transcribed in mitotic cells and is induced approximately 15-fold in meiosis. REC103 has 26% amino acid identity to the Schizasaccharomyces pombe rec14+ gene; mutations in both genes confer similar meiotic phenotypes, suggesting that they may play similar roles in meiotic recombination.</description><identifier>ISSN: 0016-6731</identifier><identifier>ISSN: 1943-2631</identifier><identifier>EISSN: 1943-2631</identifier><identifier>DOI: 10.1093/genetics/146.4.1265</identifier><identifier>PMID: 9258672</identifier><identifier>CODEN: GENTAE</identifier><language>eng</language><publisher>United States: Genetics Soc America</publisher><subject>ARN MENSAJERO ; ARN MESSAGER ; Cell division ; Chromosome Mapping ; Cloning, Molecular ; CROSSING OVER ; Crossing Over, Genetic ; CRUZAMIENTO INTERCROMOSOMICO ; ESPORAS FUNGICAS ; ESPORULACION ; EXPRESION GENICA ; EXPRESSION DES GENES ; FENOTIPOS ; FUNGAL SPORES ; GENE ; GENE EXPRESSION ; GENES ; Genes, Fungal ; GENETICA ; GENETICS ; GENETIQUE ; Investigations ; MEIOSE ; MEIOSIS ; Meiosis - genetics ; MESSENGER RNA ; MITOSE ; MITOSIS ; Mitosis - genetics ; MUTACION ; MUTATION ; PHENOTYPE ; PHENOTYPES ; REC103 GENE ; RECOMBINACION ; RECOMBINAISON ; RECOMBINATION ; Recombination, Genetic ; RESTRICTION MAPPING ; SACCHAROMYCES CEREVISIAE ; Saccharomyces cerevisiae - genetics ; Schizosaccharomyces - genetics ; SPORE FONGIQUE ; Spores, Fungal - genetics ; SPORULATION ; Yeast</subject><ispartof>Genetics (Austin), 1997-08, Vol.146 (4), p.1265-1274</ispartof><rights>Copyright Genetics Society of America Aug 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c578t-6783490b4df898b8cfec873a943bf3009be7bdbf24c3013ac342e4ac2c4792863</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9258672$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gardiner, J.M. (University of Iowa, Iowa City, IA.)</creatorcontrib><creatorcontrib>Bullard, S.A</creatorcontrib><creatorcontrib>Chrome, C</creatorcontrib><creatorcontrib>Malone, R.E</creatorcontrib><title>Molecular and genetic analysis of REC103, an early meiotic recombination gene in yeast</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>In the yeast Saccharomyces cerevisiae at least 10 genes are required to begin meiotic recombination. A new early recombination gene REC103 is described in this paper. It was initially defined by the rec103-1 mutation found in a selection for mutations overcoming the spore inviability of a rad52 spo13 haploid strain. Mutations in REC103 also rescue rad52 in spo13 diploids. rec103 spo13 strains produce viable spores; these spores show no evidence of meiotic recombination. rec103 SPO13 diploids produce no viable spores, consistent with loss of recombination. Mutations in REC103 do not affect mitotic recombination, growth, or repair. These phenotypes are identical to those conferred by mutations in several other early meiotic recombination genes (e.g., REC102, REC104, REC114, ME14, MER2, and SPO11). REC103 maps to chromosome VII between ADE5 and RAD54. Cloning and sequencing of REC103 reveals that REC103 is identical to SK18, a gene that depresses the expression of yeast double-stranded ("killer") (ds)RNA viruses. REC103/SK18 is transcribed in mitotic cells and is induced approximately 15-fold in meiosis. REC103 has 26% amino acid identity to the Schizasaccharomyces pombe rec14+ gene; mutations in both genes confer similar meiotic phenotypes, suggesting that they may play similar roles in meiotic recombination.</description><subject>ARN MENSAJERO</subject><subject>ARN MESSAGER</subject><subject>Cell division</subject><subject>Chromosome Mapping</subject><subject>Cloning, Molecular</subject><subject>CROSSING OVER</subject><subject>Crossing Over, Genetic</subject><subject>CRUZAMIENTO INTERCROMOSOMICO</subject><subject>ESPORAS FUNGICAS</subject><subject>ESPORULACION</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>FENOTIPOS</subject><subject>FUNGAL SPORES</subject><subject>GENE</subject><subject>GENE EXPRESSION</subject><subject>GENES</subject><subject>Genes, Fungal</subject><subject>GENETICA</subject><subject>GENETICS</subject><subject>GENETIQUE</subject><subject>Investigations</subject><subject>MEIOSE</subject><subject>MEIOSIS</subject><subject>Meiosis - genetics</subject><subject>MESSENGER RNA</subject><subject>MITOSE</subject><subject>MITOSIS</subject><subject>Mitosis - genetics</subject><subject>MUTACION</subject><subject>MUTATION</subject><subject>PHENOTYPE</subject><subject>PHENOTYPES</subject><subject>REC103 GENE</subject><subject>RECOMBINACION</subject><subject>RECOMBINAISON</subject><subject>RECOMBINATION</subject><subject>Recombination, Genetic</subject><subject>RESTRICTION MAPPING</subject><subject>SACCHAROMYCES CEREVISIAE</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Schizosaccharomyces - genetics</subject><subject>SPORE FONGIQUE</subject><subject>Spores, Fungal - genetics</subject><subject>SPORULATION</subject><subject>Yeast</subject><issn>0016-6731</issn><issn>1943-2631</issn><issn>1943-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9rFDEUxYModVv9BCIMPtgXd5ubZDLJiyBL_QMVQa2vIZO9s5uSmbTJjMt-e7PuWlQE85KQ87sn9-YQ8gzoAqjmF2sccPQuX4CQC7EAJusHZAZa8DmTHB6SGaUg57Lh8Jic5nxDKZW6VifkRLNayYbNyLePMaCbgk2VHVbV0bKcbdhln6vYVZ8vl0D5q3JXoU1hV_Xo4x5K6GLf-sGOPg4_Sys_VDu0eXxCHnU2ZHx63M_I9dvLr8v386tP7z4s31zNXd2osbSmuNC0FatOadUq16FTDbdlhLbjlOoWm3bVdkw4ToFbxwVDYR1zotFMSX5GXh98b6e2x5XDYUw2mNvke5t2Jlpv_lQGvzHr-N0Ao4o2vBi8PBqkeDdhHk3vs8MQ7IBxyqY8AyCY-i8IkioJAgr44i_wJk6p_Gc2bK-zsgrED5BLMeeE3X3LQM0-XPMrXFPCNcLswy1Vz3-f9r7mmGbRzw_6xq83W5_Q5N6GUGgw2-32X06djcauk8_m-gto3dBGQ835D-z3uFc</recordid><startdate>19970801</startdate><enddate>19970801</enddate><creator>Gardiner, J.M. (University of Iowa, Iowa City, IA.)</creator><creator>Bullard, S.A</creator><creator>Chrome, C</creator><creator>Malone, R.E</creator><general>Genetics Soc America</general><general>Genetics Society of America</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>4T-</scope><scope>4U-</scope><scope>7QP</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970801</creationdate><title>Molecular and genetic analysis of REC103, an early meiotic recombination gene in yeast</title><author>Gardiner, J.M. (University of Iowa, Iowa City, IA.) ; Bullard, S.A ; Chrome, C ; Malone, R.E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c578t-6783490b4df898b8cfec873a943bf3009be7bdbf24c3013ac342e4ac2c4792863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>Cell division</topic><topic>Chromosome Mapping</topic><topic>Cloning, Molecular</topic><topic>CROSSING OVER</topic><topic>Crossing Over, Genetic</topic><topic>CRUZAMIENTO INTERCROMOSOMICO</topic><topic>ESPORAS FUNGICAS</topic><topic>ESPORULACION</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>FENOTIPOS</topic><topic>FUNGAL SPORES</topic><topic>GENE</topic><topic>GENE EXPRESSION</topic><topic>GENES</topic><topic>Genes, Fungal</topic><topic>GENETICA</topic><topic>GENETICS</topic><topic>GENETIQUE</topic><topic>Investigations</topic><topic>MEIOSE</topic><topic>MEIOSIS</topic><topic>Meiosis - genetics</topic><topic>MESSENGER RNA</topic><topic>MITOSE</topic><topic>MITOSIS</topic><topic>Mitosis - genetics</topic><topic>MUTACION</topic><topic>MUTATION</topic><topic>PHENOTYPE</topic><topic>PHENOTYPES</topic><topic>REC103 GENE</topic><topic>RECOMBINACION</topic><topic>RECOMBINAISON</topic><topic>RECOMBINATION</topic><topic>Recombination, Genetic</topic><topic>RESTRICTION MAPPING</topic><topic>SACCHAROMYCES CEREVISIAE</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Schizosaccharomyces - genetics</topic><topic>SPORE FONGIQUE</topic><topic>Spores, Fungal - genetics</topic><topic>SPORULATION</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gardiner, J.M. (University of Iowa, Iowa City, IA.)</creatorcontrib><creatorcontrib>Bullard, S.A</creatorcontrib><creatorcontrib>Chrome, C</creatorcontrib><creatorcontrib>Malone, R.E</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genetics (Austin)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gardiner, J.M. (University of Iowa, Iowa City, IA.)</au><au>Bullard, S.A</au><au>Chrome, C</au><au>Malone, R.E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular and genetic analysis of REC103, an early meiotic recombination gene in yeast</atitle><jtitle>Genetics (Austin)</jtitle><addtitle>Genetics</addtitle><date>1997-08-01</date><risdate>1997</risdate><volume>146</volume><issue>4</issue><spage>1265</spage><epage>1274</epage><pages>1265-1274</pages><issn>0016-6731</issn><issn>1943-2631</issn><eissn>1943-2631</eissn><coden>GENTAE</coden><abstract>In the yeast Saccharomyces cerevisiae at least 10 genes are required to begin meiotic recombination. A new early recombination gene REC103 is described in this paper. It was initially defined by the rec103-1 mutation found in a selection for mutations overcoming the spore inviability of a rad52 spo13 haploid strain. Mutations in REC103 also rescue rad52 in spo13 diploids. rec103 spo13 strains produce viable spores; these spores show no evidence of meiotic recombination. rec103 SPO13 diploids produce no viable spores, consistent with loss of recombination. Mutations in REC103 do not affect mitotic recombination, growth, or repair. These phenotypes are identical to those conferred by mutations in several other early meiotic recombination genes (e.g., REC102, REC104, REC114, ME14, MER2, and SPO11). REC103 maps to chromosome VII between ADE5 and RAD54. Cloning and sequencing of REC103 reveals that REC103 is identical to SK18, a gene that depresses the expression of yeast double-stranded ("killer") (ds)RNA viruses. REC103/SK18 is transcribed in mitotic cells and is induced approximately 15-fold in meiosis. REC103 has 26% amino acid identity to the Schizasaccharomyces pombe rec14+ gene; mutations in both genes confer similar meiotic phenotypes, suggesting that they may play similar roles in meiotic recombination.</abstract><cop>United States</cop><pub>Genetics Soc America</pub><pmid>9258672</pmid><doi>10.1093/genetics/146.4.1265</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Genetics (Austin), 1997-08, Vol.146 (4), p.1265-1274 |
| issn | 0016-6731 1943-2631 1943-2631 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1208073 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | ARN MENSAJERO ARN MESSAGER Cell division Chromosome Mapping Cloning, Molecular CROSSING OVER Crossing Over, Genetic CRUZAMIENTO INTERCROMOSOMICO ESPORAS FUNGICAS ESPORULACION EXPRESION GENICA EXPRESSION DES GENES FENOTIPOS FUNGAL SPORES GENE GENE EXPRESSION GENES Genes, Fungal GENETICA GENETICS GENETIQUE Investigations MEIOSE MEIOSIS Meiosis - genetics MESSENGER RNA MITOSE MITOSIS Mitosis - genetics MUTACION MUTATION PHENOTYPE PHENOTYPES REC103 GENE RECOMBINACION RECOMBINAISON RECOMBINATION Recombination, Genetic RESTRICTION MAPPING SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - genetics Schizosaccharomyces - genetics SPORE FONGIQUE Spores, Fungal - genetics SPORULATION Yeast |
| title | Molecular and genetic analysis of REC103, an early meiotic recombination gene in yeast |
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