stoned locus of Drosophila melanogaster produces a dicistronic transcript and encodes two distinct polypeptides
The stoned gene of Drosophila melanogaster is required for normal neuronal function in both adult and larva. We have identified DNA sequences that lie within a genetic region that is known to include the stoned gene and that also reveal restriction site variations in two stoned lethal mutants. This...
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| Veröffentlicht in: | Genetics (Austin) 1996-08, Vol.143 (4), p.1699-1711 |
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| creator | Andrews, J Smith, M Merakovsky, J Coulson, M Hannan, F Kelly, L.E |
| description | The stoned gene of Drosophila melanogaster is required for normal neuronal function in both adult and larva. We have identified DNA sequences that lie within a genetic region that is known to include the stoned gene and that also reveal restriction site variations in two stoned lethal mutants. This genomic region contains a single transcription unit coding for an approximate 8.4kb transcript. The transcript is preferentially expressed in the head of adult flies. The isolation and sequencing of cDNA and genomic clones reveals that stoned appears to encode a dicistronic mRNA, although the possible existence of other forms of mRNA cannot be excluded. Antibody cross-reactivity shows that two proteins are translated from the stoned locus in vivo. Both open reading frames (ORFs) encode novel proteins. The protein encoded by the first ORF contains four tandemly repeated motifs, and one domain of the protein encoded by the second ORF shows similarity to a family of proteins (AP50s) associated with clathrin assembly protein complexes. |
| doi_str_mv | 10.1093/genetics/143.4.1699 |
| format | Article |
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We have identified DNA sequences that lie within a genetic region that is known to include the stoned gene and that also reveal restriction site variations in two stoned lethal mutants. This genomic region contains a single transcription unit coding for an approximate 8.4kb transcript. The transcript is preferentially expressed in the head of adult flies. The isolation and sequencing of cDNA and genomic clones reveals that stoned appears to encode a dicistronic mRNA, although the possible existence of other forms of mRNA cannot be excluded. Antibody cross-reactivity shows that two proteins are translated from the stoned locus in vivo. Both open reading frames (ORFs) encode novel proteins. The protein encoded by the first ORF contains four tandemly repeated motifs, and one domain of the protein encoded by the second ORF shows similarity to a family of proteins (AP50s) associated with clathrin assembly protein complexes.</description><identifier>ISSN: 0016-6731</identifier><identifier>ISSN: 1943-2631</identifier><identifier>EISSN: 1943-2631</identifier><identifier>DOI: 10.1093/genetics/143.4.1699</identifier><identifier>PMID: 8844157</identifier><identifier>CODEN: GENTAE</identifier><language>eng</language><publisher>United States: Genetics Soc America</publisher><subject>Amino Acid Sequence ; amino acid sequences ; animal proteins ; Animals ; Base Sequence ; Cloning, Molecular ; complementary DNA ; Deoxyribonucleic acid ; dicistronic messenger rna ; DNA ; DNA, Complementary - genetics ; Drosophila melanogaster ; Drosophila melanogaster - genetics ; Drosophila melanogaster - physiology ; Female ; genbank/u54982 ; Genes, Insect ; Genetic Complementation Test ; Genetics ; Immunochemistry ; Insects ; introns ; Investigations ; loci ; Male ; messenger RNA ; Molecular Sequence Data ; Nervous System Physiological Phenomena ; nucleotide sequences ; Open Reading Frames ; polypeptides ; Proteins - genetics ; Proteins - immunology ; Proteins - physiology ; Restriction Mapping ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sequence Homology, Amino Acid ; structural genes ; Transcription, Genetic</subject><ispartof>Genetics (Austin), 1996-08, Vol.143 (4), p.1699-1711</ispartof><rights>Copyright Genetics Society of America Aug 1996</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c514t-db3fe487ad187ca196a4bdbeb20c6a576db020d98ba028c911aa60424e2bf9f43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8844157$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Andrews, J</creatorcontrib><creatorcontrib>Smith, M</creatorcontrib><creatorcontrib>Merakovsky, J</creatorcontrib><creatorcontrib>Coulson, M</creatorcontrib><creatorcontrib>Hannan, F</creatorcontrib><creatorcontrib>Kelly, L.E</creatorcontrib><title>stoned locus of Drosophila melanogaster produces a dicistronic transcript and encodes two distinct polypeptides</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>The stoned gene of Drosophila melanogaster is required for normal neuronal function in both adult and larva. We have identified DNA sequences that lie within a genetic region that is known to include the stoned gene and that also reveal restriction site variations in two stoned lethal mutants. This genomic region contains a single transcription unit coding for an approximate 8.4kb transcript. The transcript is preferentially expressed in the head of adult flies. The isolation and sequencing of cDNA and genomic clones reveals that stoned appears to encode a dicistronic mRNA, although the possible existence of other forms of mRNA cannot be excluded. Antibody cross-reactivity shows that two proteins are translated from the stoned locus in vivo. Both open reading frames (ORFs) encode novel proteins. The protein encoded by the first ORF contains four tandemly repeated motifs, and one domain of the protein encoded by the second ORF shows similarity to a family of proteins (AP50s) associated with clathrin assembly protein complexes.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>animal proteins</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>complementary DNA</subject><subject>Deoxyribonucleic acid</subject><subject>dicistronic messenger rna</subject><subject>DNA</subject><subject>DNA, Complementary - genetics</subject><subject>Drosophila melanogaster</subject><subject>Drosophila melanogaster - genetics</subject><subject>Drosophila melanogaster - physiology</subject><subject>Female</subject><subject>genbank/u54982</subject><subject>Genes, Insect</subject><subject>Genetic Complementation Test</subject><subject>Genetics</subject><subject>Immunochemistry</subject><subject>Insects</subject><subject>introns</subject><subject>Investigations</subject><subject>loci</subject><subject>Male</subject><subject>messenger RNA</subject><subject>Molecular Sequence Data</subject><subject>Nervous System Physiological Phenomena</subject><subject>nucleotide sequences</subject><subject>Open Reading Frames</subject><subject>polypeptides</subject><subject>Proteins - genetics</subject><subject>Proteins - immunology</subject><subject>Proteins - physiology</subject><subject>Restriction Mapping</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><subject>structural genes</subject><subject>Transcription, Genetic</subject><issn>0016-6731</issn><issn>1943-2631</issn><issn>1943-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9vFCEYhydGU7fVT2CMxEM97ZYXmAEuTUz9mzTxoD0TBphdmlkYgXHSby_Nro314onD73l_eXmfpnkFeANY0outC654ky-A0Q3bQCflk2YFktE16Sg8bVYYQ7fuOIXnzWnOtxjjTrbipDkRgjFo-aqJucTgLBqjmTOKA_qQYo7Tzo8a7d2oQ9zqXFxCU4p2Ni4jjaw3PpcUgzeoJB2ySX4qSAeLXDDRVqgssWK5-GAKmuJ4N7mp-Jq8aJ4Neszu5fE9a24-ffxx9WV9_e3z16v312vTAitr29PBMcG1BcGNBtlp1tve9QSbTre8sz0m2ErRa0yEkQBad5gR5kg_yIHRs-by0DvN_d5Z40LddFRT8nud7lTUXj1Ogt-pbfylgGDOKKkF58eCFH_OLhe199m4sZ7ExTkrLhhmEuR_QWgFb7mACr79B7yNcwr1CooAA9oRwStED5CpHnJyw8PKgNW9dfXHuqrWFVP31uvU679_-zBz1Fzzd4d857e7xSen8l6PY6VBLcvyqOnNgRx0VHqbfFY33wkGiqElQLigvwFOMcWD</recordid><startdate>19960801</startdate><enddate>19960801</enddate><creator>Andrews, J</creator><creator>Smith, M</creator><creator>Merakovsky, J</creator><creator>Coulson, M</creator><creator>Hannan, F</creator><creator>Kelly, L.E</creator><general>Genetics Soc America</general><general>Genetics Society of America</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>4T-</scope><scope>4U-</scope><scope>7QP</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19960801</creationdate><title>stoned locus of Drosophila melanogaster produces a dicistronic transcript and encodes two distinct polypeptides</title><author>Andrews, J ; Smith, M ; Merakovsky, J ; Coulson, M ; Hannan, F ; Kelly, L.E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c514t-db3fe487ad187ca196a4bdbeb20c6a576db020d98ba028c911aa60424e2bf9f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>animal proteins</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>complementary DNA</topic><topic>Deoxyribonucleic acid</topic><topic>dicistronic messenger rna</topic><topic>DNA</topic><topic>DNA, Complementary - genetics</topic><topic>Drosophila melanogaster</topic><topic>Drosophila melanogaster - genetics</topic><topic>Drosophila melanogaster - physiology</topic><topic>Female</topic><topic>genbank/u54982</topic><topic>Genes, Insect</topic><topic>Genetic Complementation Test</topic><topic>Genetics</topic><topic>Immunochemistry</topic><topic>Insects</topic><topic>introns</topic><topic>Investigations</topic><topic>loci</topic><topic>Male</topic><topic>messenger RNA</topic><topic>Molecular Sequence Data</topic><topic>Nervous System Physiological Phenomena</topic><topic>nucleotide sequences</topic><topic>Open Reading Frames</topic><topic>polypeptides</topic><topic>Proteins - genetics</topic><topic>Proteins - immunology</topic><topic>Proteins - physiology</topic><topic>Restriction Mapping</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><topic>structural genes</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Andrews, J</creatorcontrib><creatorcontrib>Smith, M</creatorcontrib><creatorcontrib>Merakovsky, J</creatorcontrib><creatorcontrib>Coulson, M</creatorcontrib><creatorcontrib>Hannan, F</creatorcontrib><creatorcontrib>Kelly, L.E</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genetics (Austin)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Andrews, J</au><au>Smith, M</au><au>Merakovsky, J</au><au>Coulson, M</au><au>Hannan, F</au><au>Kelly, L.E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>stoned locus of Drosophila melanogaster produces a dicistronic transcript and encodes two distinct polypeptides</atitle><jtitle>Genetics (Austin)</jtitle><addtitle>Genetics</addtitle><date>1996-08-01</date><risdate>1996</risdate><volume>143</volume><issue>4</issue><spage>1699</spage><epage>1711</epage><pages>1699-1711</pages><issn>0016-6731</issn><issn>1943-2631</issn><eissn>1943-2631</eissn><coden>GENTAE</coden><abstract>The stoned gene of Drosophila melanogaster is required for normal neuronal function in both adult and larva. We have identified DNA sequences that lie within a genetic region that is known to include the stoned gene and that also reveal restriction site variations in two stoned lethal mutants. This genomic region contains a single transcription unit coding for an approximate 8.4kb transcript. The transcript is preferentially expressed in the head of adult flies. The isolation and sequencing of cDNA and genomic clones reveals that stoned appears to encode a dicistronic mRNA, although the possible existence of other forms of mRNA cannot be excluded. Antibody cross-reactivity shows that two proteins are translated from the stoned locus in vivo. Both open reading frames (ORFs) encode novel proteins. 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| ispartof | Genetics (Austin), 1996-08, Vol.143 (4), p.1699-1711 |
| issn | 0016-6731 1943-2631 1943-2631 |
| language | eng |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence amino acid sequences animal proteins Animals Base Sequence Cloning, Molecular complementary DNA Deoxyribonucleic acid dicistronic messenger rna DNA DNA, Complementary - genetics Drosophila melanogaster Drosophila melanogaster - genetics Drosophila melanogaster - physiology Female genbank/u54982 Genes, Insect Genetic Complementation Test Genetics Immunochemistry Insects introns Investigations loci Male messenger RNA Molecular Sequence Data Nervous System Physiological Phenomena nucleotide sequences Open Reading Frames polypeptides Proteins - genetics Proteins - immunology Proteins - physiology Restriction Mapping RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Homology, Amino Acid structural genes Transcription, Genetic |
| title | stoned locus of Drosophila melanogaster produces a dicistronic transcript and encodes two distinct polypeptides |
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