An integrated genetic map of the African human Malaria vector mosquito, Anopheles gambiae
We present a genetic map based on microsatellite polymorphisms for the African human malaria vector, Anopheles gambiae. Polymorphisms in laboratory strains were detected for 89% of the tested microsatellite markers. Genotyping was performed for individual mosquitoes from 13 backcross families that i...
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| Veröffentlicht in: | Genetics (Austin) 1996-06, Vol.143 (2), p.941-952 |
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| creator | Zheng, L Benedict, M. Q Cornel, A. J Collins, F. H Kafatos, F. C |
| description | We present a genetic map based on microsatellite polymorphisms for the African human malaria vector, Anopheles gambiae. Polymorphisms in laboratory strains were detected for 89% of the tested microsatellite markers. Genotyping was performed for individual mosquitoes from 13 backcross families that included 679 progeny. Three linkage groups were identified, corresponding to the three chromosomes. We added 22 new markers to the existing X chromosome map, for a total of 46 microsatellite markers spanning a distance of 48.9 cM. The second chromosome has 57 and the third 28 microsatellite markers spanning a distance of 72.4 and 93.7 cM, respectively. The overall average distance between markers is 1.6 cM (or 1.1,1.2, and 3.2 cM for the X, second, and third chromosomes, respectively). In addition to the 131 microsatellite markers, the current map also includes a biochemical selectable marker, Dieldrin resistance (Dl), on the second chromosome and five visible markers, pink-eye (p) and white (w) on the X, collarless (c) and lunate (lu) on the second, and red-eye (r) on the third. The cytogenetic locations on the nurse cell polytene chromosomes have been determined for 47 markers, making this map an integrated tool for cytogenetic, genetic, and molecular analysis. |
| doi_str_mv | 10.1093/genetics/143.2.941 |
| format | Article |
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Q ; Cornel, A. J ; Collins, F. H ; Kafatos, F. C</creator><creatorcontrib>Zheng, L ; Benedict, M. Q ; Cornel, A. J ; Collins, F. H ; Kafatos, F. C ; South African Water Research Commission, Pretoria (South Africa) ; Harvard University, Cambridge, MA</creatorcontrib><description>We present a genetic map based on microsatellite polymorphisms for the African human malaria vector, Anopheles gambiae. Polymorphisms in laboratory strains were detected for 89% of the tested microsatellite markers. Genotyping was performed for individual mosquitoes from 13 backcross families that included 679 progeny. Three linkage groups were identified, corresponding to the three chromosomes. We added 22 new markers to the existing X chromosome map, for a total of 46 microsatellite markers spanning a distance of 48.9 cM. The second chromosome has 57 and the third 28 microsatellite markers spanning a distance of 72.4 and 93.7 cM, respectively. The overall average distance between markers is 1.6 cM (or 1.1,1.2, and 3.2 cM for the X, second, and third chromosomes, respectively). In addition to the 131 microsatellite markers, the current map also includes a biochemical selectable marker, Dieldrin resistance (Dl), on the second chromosome and five visible markers, pink-eye (p) and white (w) on the X, collarless (c) and lunate (lu) on the second, and red-eye (r) on the third. The cytogenetic locations on the nurse cell polytene chromosomes have been determined for 47 markers, making this map an integrated tool for cytogenetic, genetic, and molecular analysis.</description><identifier>ISSN: 0016-6731</identifier><identifier>ISSN: 1943-2631</identifier><identifier>EISSN: 1943-2631</identifier><identifier>DOI: 10.1093/genetics/143.2.941</identifier><identifier>PMID: 8725240</identifier><identifier>CODEN: GENTAE</identifier><language>eng</language><publisher>United States: Genetics Soc America</publisher><subject>adn ; Animals ; Anopheles - genetics ; anopheles gambiae ; carte genetique ; chromosome ; Chromosome Mapping ; chromosomes ; cromosomas ; dna ; dna hybridization ; gene ; genes ; genetic maps ; Genetic Markers ; genetic polymorphism ; Genetics ; hibridacion de adn ; Humans ; hybridation d' adn ; Investigations ; Malaria ; Malaria - parasitology ; mapas geneticos ; marcadores geneticos ; marqueur genetique ; Mosquitoes ; nucleic probes ; nucleotide sequence ; polimorfismo genetico ; polymorphisme genetique ; secuencia nucleotidica ; sequence nucleotidique ; sondas nucleicas ; sonde nucleique</subject><ispartof>Genetics (Austin), 1996-06, Vol.143 (2), p.941-952</ispartof><rights>Copyright Genetics Society of America Jun 1996</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-19654493ac7121412cca1410d9f7cf4c4500869dadff810adf427d22f64ac1a23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8725240$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zheng, L</creatorcontrib><creatorcontrib>Benedict, M. Q</creatorcontrib><creatorcontrib>Cornel, A. J</creatorcontrib><creatorcontrib>Collins, F. H</creatorcontrib><creatorcontrib>Kafatos, F. C</creatorcontrib><creatorcontrib>South African Water Research Commission, Pretoria (South Africa)</creatorcontrib><creatorcontrib>Harvard University, Cambridge, MA</creatorcontrib><title>An integrated genetic map of the African human Malaria vector mosquito, Anopheles gambiae</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>We present a genetic map based on microsatellite polymorphisms for the African human malaria vector, Anopheles gambiae. Polymorphisms in laboratory strains were detected for 89% of the tested microsatellite markers. Genotyping was performed for individual mosquitoes from 13 backcross families that included 679 progeny. Three linkage groups were identified, corresponding to the three chromosomes. We added 22 new markers to the existing X chromosome map, for a total of 46 microsatellite markers spanning a distance of 48.9 cM. The second chromosome has 57 and the third 28 microsatellite markers spanning a distance of 72.4 and 93.7 cM, respectively. The overall average distance between markers is 1.6 cM (or 1.1,1.2, and 3.2 cM for the X, second, and third chromosomes, respectively). In addition to the 131 microsatellite markers, the current map also includes a biochemical selectable marker, Dieldrin resistance (Dl), on the second chromosome and five visible markers, pink-eye (p) and white (w) on the X, collarless (c) and lunate (lu) on the second, and red-eye (r) on the third. The cytogenetic locations on the nurse cell polytene chromosomes have been determined for 47 markers, making this map an integrated tool for cytogenetic, genetic, and molecular analysis.</description><subject>adn</subject><subject>Animals</subject><subject>Anopheles - genetics</subject><subject>anopheles gambiae</subject><subject>carte genetique</subject><subject>chromosome</subject><subject>Chromosome Mapping</subject><subject>chromosomes</subject><subject>cromosomas</subject><subject>dna</subject><subject>dna hybridization</subject><subject>gene</subject><subject>genes</subject><subject>genetic maps</subject><subject>Genetic Markers</subject><subject>genetic polymorphism</subject><subject>Genetics</subject><subject>hibridacion de adn</subject><subject>Humans</subject><subject>hybridation d' adn</subject><subject>Investigations</subject><subject>Malaria</subject><subject>Malaria - parasitology</subject><subject>mapas geneticos</subject><subject>marcadores geneticos</subject><subject>marqueur genetique</subject><subject>Mosquitoes</subject><subject>nucleic probes</subject><subject>nucleotide sequence</subject><subject>polimorfismo genetico</subject><subject>polymorphisme genetique</subject><subject>secuencia nucleotidica</subject><subject>sequence nucleotidique</subject><subject>sondas nucleicas</subject><subject>sonde nucleique</subject><issn>0016-6731</issn><issn>1943-2631</issn><issn>1943-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUtv1DAUhS1EVYbCH0ACWSxYkalfie0N0qjiJRWxgC5YWbeOnbhK4qmddMS_x3SG0nbjs_B3j869B6FXlKwp0fy0c5Obg82nVPA1W2tBn6AV1YJXrOH0KVoRQpuqkZw-Q89zviKENLpWx-hYSVYzQVbo12bCYZpdl2B2LT444hG2OHo89w5vfAoWJtwvY3m_wQApAL5xdo4JjzFfL2GO7_FmitveDS7jDsbLAO4FOvIwZPfyoCfo4tPHn2dfqvPvn7-ebc4rK6SaK6qbWgjNwUrKqKDMWihCWu2l9cKKmhDV6BZa7xUlRQSTLWO-EWApMH6CPux9t8vl6FrrpjnBYLYpjJB-mwjBPPyZQm-6eGMoI5LXpBi8OxikeL24PJsxZOuGASYXl2ykYoqqWhXw7SPwKi5pKsuZ2-TltrpAbA_ZFHNOzt8locT8bc38a82U1gwzpbUy9Pr-Dncjh5r-R-xD1-9CciaPMAyFpma32903erMHPUQDXQrZXPygWktCuGyk4n8Aw8KsJQ</recordid><startdate>19960601</startdate><enddate>19960601</enddate><creator>Zheng, L</creator><creator>Benedict, M. Q</creator><creator>Cornel, A. J</creator><creator>Collins, F. H</creator><creator>Kafatos, F. C</creator><general>Genetics Soc America</general><general>Genetics Society of America</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>4T-</scope><scope>4U-</scope><scope>7QP</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19960601</creationdate><title>An integrated genetic map of the African human Malaria vector mosquito, Anopheles gambiae</title><author>Zheng, L ; Benedict, M. Q ; Cornel, A. J ; Collins, F. H ; Kafatos, F. C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-19654493ac7121412cca1410d9f7cf4c4500869dadff810adf427d22f64ac1a23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>adn</topic><topic>Animals</topic><topic>Anopheles - genetics</topic><topic>anopheles gambiae</topic><topic>carte genetique</topic><topic>chromosome</topic><topic>Chromosome Mapping</topic><topic>chromosomes</topic><topic>cromosomas</topic><topic>dna</topic><topic>dna hybridization</topic><topic>gene</topic><topic>genes</topic><topic>genetic maps</topic><topic>Genetic Markers</topic><topic>genetic polymorphism</topic><topic>Genetics</topic><topic>hibridacion de adn</topic><topic>Humans</topic><topic>hybridation d' adn</topic><topic>Investigations</topic><topic>Malaria</topic><topic>Malaria - parasitology</topic><topic>mapas geneticos</topic><topic>marcadores geneticos</topic><topic>marqueur genetique</topic><topic>Mosquitoes</topic><topic>nucleic probes</topic><topic>nucleotide sequence</topic><topic>polimorfismo genetico</topic><topic>polymorphisme genetique</topic><topic>secuencia nucleotidica</topic><topic>sequence nucleotidique</topic><topic>sondas nucleicas</topic><topic>sonde nucleique</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zheng, L</creatorcontrib><creatorcontrib>Benedict, M. Q</creatorcontrib><creatorcontrib>Cornel, A. J</creatorcontrib><creatorcontrib>Collins, F. H</creatorcontrib><creatorcontrib>Kafatos, F. C</creatorcontrib><creatorcontrib>South African Water Research Commission, Pretoria (South Africa)</creatorcontrib><creatorcontrib>Harvard University, Cambridge, MA</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genetics (Austin)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zheng, L</au><au>Benedict, M. Q</au><au>Cornel, A. J</au><au>Collins, F. H</au><au>Kafatos, F. C</au><aucorp>South African Water Research Commission, Pretoria (South Africa)</aucorp><aucorp>Harvard University, Cambridge, MA</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An integrated genetic map of the African human Malaria vector mosquito, Anopheles gambiae</atitle><jtitle>Genetics (Austin)</jtitle><addtitle>Genetics</addtitle><date>1996-06-01</date><risdate>1996</risdate><volume>143</volume><issue>2</issue><spage>941</spage><epage>952</epage><pages>941-952</pages><issn>0016-6731</issn><issn>1943-2631</issn><eissn>1943-2631</eissn><coden>GENTAE</coden><abstract>We present a genetic map based on microsatellite polymorphisms for the African human malaria vector, Anopheles gambiae. Polymorphisms in laboratory strains were detected for 89% of the tested microsatellite markers. Genotyping was performed for individual mosquitoes from 13 backcross families that included 679 progeny. Three linkage groups were identified, corresponding to the three chromosomes. We added 22 new markers to the existing X chromosome map, for a total of 46 microsatellite markers spanning a distance of 48.9 cM. The second chromosome has 57 and the third 28 microsatellite markers spanning a distance of 72.4 and 93.7 cM, respectively. The overall average distance between markers is 1.6 cM (or 1.1,1.2, and 3.2 cM for the X, second, and third chromosomes, respectively). In addition to the 131 microsatellite markers, the current map also includes a biochemical selectable marker, Dieldrin resistance (Dl), on the second chromosome and five visible markers, pink-eye (p) and white (w) on the X, collarless (c) and lunate (lu) on the second, and red-eye (r) on the third. The cytogenetic locations on the nurse cell polytene chromosomes have been determined for 47 markers, making this map an integrated tool for cytogenetic, genetic, and molecular analysis.</abstract><cop>United States</cop><pub>Genetics Soc America</pub><pmid>8725240</pmid><doi>10.1093/genetics/143.2.941</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Genetics (Austin), 1996-06, Vol.143 (2), p.941-952 |
| issn | 0016-6731 1943-2631 1943-2631 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | adn Animals Anopheles - genetics anopheles gambiae carte genetique chromosome Chromosome Mapping chromosomes cromosomas dna dna hybridization gene genes genetic maps Genetic Markers genetic polymorphism Genetics hibridacion de adn Humans hybridation d' adn Investigations Malaria Malaria - parasitology mapas geneticos marcadores geneticos marqueur genetique Mosquitoes nucleic probes nucleotide sequence polimorfismo genetico polymorphisme genetique secuencia nucleotidica sequence nucleotidique sondas nucleicas sonde nucleique |
| title | An integrated genetic map of the African human Malaria vector mosquito, Anopheles gambiae |
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